Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 6 of 6 for “"ribosome inactivating protein"”.
-
Characterization and Expression of Plant Defense Genes in Peach and Tomato
… a maize beta-glucanase, maize chitinase, iris ribosome-inactivating protein (RIP), or an antimicrobial protein gene, respectively, each driven by the CaMV 35S promoter. Transformation was initially verified by the presence of an nptII gene and GUS enzyme activity in the uidA reporter gene, both …
-
Cell Targeted Ribosome Inactivating Proteins Derived from Protein Combinatorial Libraries
Combinatorial protein libraries based on a protein template offer a vast potential for deriving protein variants harboring new receptor specificity while retaining other tem-plate functions to serve as library search-engines, cell-routing sequences and therapeutic domains. This concept was tested …
-
The role of a pore-forming protein, Mpeg1, in cytosolic import
… The main aim of my PhD was to identify proteins that play a role in the cytosolic import of antigens during cross-presentation. To do so we developed a new assay, which relies on the type I ribosome inactivating protein, saporin, which can inhibit translation upon entry into the cytosol. …
-
Characterization of endocytic escape pathway and its role in adaptive immunity
… cytosol, and further processed like endogenous proteins for presentation on MHC class I. Endocytic escape of antigens appears to be the rate-limiting step of cross-presentation, yet we know very little about the molecular mechanisms involved. To identify the machinery involved in endocytic …
-
Reversibly neutralized perfringolysin O for intracellular delivery of macromolecules/
… bases of disease, macromolecules such as proteins and siRNA can potentially be used as therapeutics, modulating biological function with high specificity to reverse pathological progression. However, while certain contexts require manipulating the biology inside the cell, the large size …