Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"ribosome binding site"”.
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An E. coli small RNA inhibits translation initiation from a distance
… regulator; it competes with the initiating ribosomes while the chaperone protein Hfq plays a supporting role. Contrary to this widely accepted model, there are a few examples in the literature where the sRNA base pairs far from the SD region, yet translation of the target mRNA is still …
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Isolation and Biochemical Characterization of an Alpha-Amylase From Lactobacillus Amylovorus and Sequencing of the Corresponding Gene
… pLPCR2 was determined. Putative promoter, ribosome binding site, and leader signal sequences were identified. The G + C content of the coding sequence for the $\alpha$-amylase gene was found to be 38%, a value within the range found for other lactobacilli strains. The sequence of the …
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Microbiology of periodontal disease in Singapore and the discovery of a bacterial intron in Actinobacillus actinomycetemcomitans
… 5a?? exons but shared the same 3a?? exon with ribosome-binding site present upstream. Conservation of splice-site consensus sequence was important for accurate splicing. The a??catalytic corea?? was a 0.56 kb RNA segment located within the 2.1 kb precursor RNA, capable of splicing heterologous …
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Activities of Bacteroides Conjugative Transposons: CTnGERM1 and CTnDOT
… of excision and transfer functions. TetQ, a ribosome protection type of Tc resistance protein, actually reduced operon expression, possibly by interacting with ribosomes that are translating the tetQ message. Computer analysis of the region upstream of the tetQ start codon predicted that the …
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Gene Expression in Archaea: Studies of Transcriptional Promoters, Messenger RNA Processing, and Five Prime Untranslated Regions in Methanocaldococcus Jannashchii
… promoter sequence and transcription factor binding affinity. Much of gene expression in M. jannaschii may be determined by intrinsic promoter strength. Messenger RNA processing is common in M. jannaschii. RNA cleavage occurs in the upstream regions of ∼20% of the protein-coding genes …
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Redefining the Coordination of Gene Expression Machineries in Bacillus subtilis
… of bacterial gene expression. As soon as the ribosome binding site emerges from the RNAP, the ribosome can initiate translation, and both physically associate and kinetically coordinate with the RNAP over the course of transcription. This close proximity between the RNAP and ribosome allows …
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Characterization of pigment production by Escherichia Coli containing a cloned Rhodococcus gene
… production in E. coli. This gene had a putative ribosome binding site and coded for an enzyme of Mr 42,560. Deletion analysis and in vitro transcription-translation experiments supported the hypothesis that pigment production in E. coli was due to a single enzyme. The gene and gene product did …
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RNA tools for optimization of multi-protein genetic systems
… sequences that can be inserted upstream of a ribosome-binding site to generate up to 175-fold repression when maximally expressed. By controlling sRNA expression, the targeted gene can be tunably repressed from 1.6- to 121-fold. We then built a pool where each of the six sRNAs was placed under …
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ROLE OF CRC IN THE REGULATION OF ALGINATE IN PSEUDOMONAS AERUGINOSA
… sigma factor, anti-sigmas and several DNA-binding transcriptional regulators. Here we examined the possibility that the catabolic repression control (Crc) protein repressor may affect alginate gene expression. A putative Crc binding site was observed adjacent to the ribosome binding site of …
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Characterisation and secretion mechanism of Bordetella pertussis autotransporter proteins
… other similar regions such as the RGD (integrin-binding) and SGXG (glycosaminoglycan-binding) motifs. The bap-5 gene also exists in B. bronchiseptica and B. parapertussis. Characteristic upstream regulatory sequences such as a ribosome-binding site were not seen in bap-5, but a potential …
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Understanding small RNA-mediated post-transcriptional regulation of Salmonella pathogenicity island 1 activator HilA in Salmonella typhimurium
… and Stnc3630, the results also defined sRNA InvR binding site within the hilA ribosome binding site region. Further genetic and molecular characterization showed that InvR directly base-pairs with hilA mRNA to prevent translation initiation, functioning to counteract HilA overactivation by HilD. …
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Microbial synthesis of antimalarial compound FR-900098: pathway characterization and engineering
… including Plasmodium falciparum, the parasite responsible for the most virulent form of the disease. The biosynthetic cluster for FR-900098 has recently been cloned from Streptomyces rubellomurinus and heterologously expressed in Streptomyces lividans and Escherichia coli. In this work, we …
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Regulation of the Mannose PTS Operon by the small RNA SgrS
… We further demonstrate that SgrS binds to two sites on the manXYZ mRNA to coordinately down-regulate translation of all the cistrons, a mechanism reminiscent of that used by the eukaryotic miRNA, lin-4 for regulation of the lin-14 mRNA. We tested the hypothesis that regulation of manY and manZ …
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The Sequence Landscape of Bacterial Genes is Shaped by Long-Range mRNA Folding
… and translation rates is accessibility of the ribosome binding site (RBS) to the ribosome. Sequences in the mRNA’s 5´ untranslated region (UTR) complementary to the RBS can decrease gene expression by base pairing and occluding ribosomes from binding. To determine whether such antagonistic …
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Investigation of the role of the extracellular β-agarase, produced by the bacterial epiphyte Pseudoalteromonas sp. LS2i, in the virulence response towards the agarophyte Gracilaria gracilis
… 1062 bp ORF, putative promoter region, putative ribosome binding site and putative transcriptional start point of the cloned agarase gene. The ORF showed sequence identity to several other β-agarases and was identified as a member of the GH-16 family of glycoside hydrolases. The agarase was …
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Biochemical Characterization of Binding Partners of Two Hsp70 Co-Chaperones In Saccharomyces Cerevisiae
… helps maintain them in a functional state. Requisite co-chaperones, including nucleotide exchange factors (NEFs) strictly regulate and serve to recruit Hsp70 to distinct cellular processes or locations. In yeast and human cells, three structurally non-related cytosolic NEFs are present: Sse1 …
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Biological control of a plant pathogen and pest by expression of a cloned Serratia marcescens chiA gene and Bacillus thuringiensis cryIA(c) gene in endophytic bacteria
… with a distance of 20 bases between the ribosome binding site of this vector and the A TG start codon of the gene. Gene expression of chiA carried on pTC33 was shown to be approximately 16-foldhigher than that of ptacchiA. This clearly illustrated that the distance between the Shine …
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Molecular analysis of two cellulase genes from Ruminococcus flavefaciens FD-1 and their transcriptional regulation
… preceded by a GAGG sequence, predicted to be a ribosome binding site. The derived amino acid sequence corresponded to a protein of Mᵣ 38686. SDS-PAGE analysis of in vitro and in vivo translational products showed that CelA has a molecular mass of ca 39 kDa and was secreted into the Escherichia …
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Optimisation of use of waste materials by Rhodopseudomonas palustris
… less effective. A combination of promoter and ribosome binding site is reported that directs a high level of gene expression; this is a useful new tool for future genetic engineering attempts. Finally, the metabolism of glycerol as a carbon source is investigated using flux analysis, showing …
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Integration of small RNAs in the control of Salmonella pathogenicity island 1(SPI1)
… for translational inhibition of hilA. The InvR binding region on the 5’ UTR of hilA is far upstream from the ribosome binding site (RBS), and we assume that InvR binding at the 5’ UTR of hilA induces structural changes in the hilA UTR to form an inhibitory structure that leads to translational …
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