Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 39 for “"ribosome binding"”.
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Thermodynamics of λ-PCR Primer Design and Effective Ribosome Binding Sites
… cloning reactions. Additionally, synthetic ribosome binding sites (RBS) were included in the primer designs to test heterologous protein expression of the cyan fluorescent reporter with different RBS strengths. These RBS sequences were designed with an online tool, the RBS Calculator. A …
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Gene Expression in Archaea: Studies of Transcriptional Promoters, Messenger RNA Processing, and Five Prime Untranslated Regions in Methanocaldococcus Jannashchii
… promoter sequence and transcription factor binding affinity. Much of gene expression in M. jannaschii may be determined by intrinsic promoter strength. Messenger RNA processing is common in M. jannaschii. RNA cleavage occurs in the upstream regions of ∼20% of the protein-coding genes …
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An E. coli small RNA inhibits translation initiation from a distance
… regulator; it competes with the initiating ribosomes while the chaperone protein Hfq plays a supporting role. Contrary to this widely accepted model, there are a few examples in the literature where the sRNA base pairs far from the SD region, yet translation of the target mRNA is still …
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Isolation and Biochemical Characterization of an Alpha-Amylase From Lactobacillus Amylovorus and Sequencing of the Corresponding Gene
… pLPCR2 was determined. Putative promoter, ribosome binding site, and leader signal sequences were identified. The G + C content of the coding sequence for the $\alpha$-amylase gene was found to be 38%, a value within the range found for other lactobacilli strains. The sequence of the …
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The Sequence Landscape of Bacterial Genes is Shaped by Long-Range mRNA Folding
… and translation rates is accessibility of the ribosome binding site (RBS) to the ribosome. Sequences in the mRNA’s 5´ untranslated region (UTR) complementary to the RBS can decrease gene expression by base pairing and occluding ribosomes from binding. To determine whether such antagonistic …
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Microbiology of periodontal disease in Singapore and the discovery of a bacterial intron in Actinobacillus actinomycetemcomitans
… 5a?? exons but shared the same 3a?? exon with ribosome-binding site present upstream. Conservation of splice-site consensus sequence was important for accurate splicing. The a??catalytic corea?? was a 0.56 kb RNA segment located within the 2.1 kb precursor RNA, capable of splicing heterologous …
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Characterization of features of the initiation of translation of satellite tobacco necrosis virus RNA
… the normal internal initiation codon. This first ribosome binding frees the upstream site from secondary structure allowing all subsequent ribosomes to initiate translation at the upstream AUG codon.
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Mechanisms of Translational Control
… mechanisms of translational control in ribosomes. Specifically, we examine the molecular basis of Sec stop codon suppression and insertion, as well as the phenotypic consequences of perturbations to this process. Additionally, we explore how ribosome-binding antibiotics, particularly the …
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Activities of Bacteroides Conjugative Transposons: CTnGERM1 and CTnDOT
… of excision and transfer functions. TetQ, a ribosome protection type of Tc resistance protein, actually reduced operon expression, possibly by interacting with ribosomes that are translating the tetQ message. Computer analysis of the region upstream of the tetQ start codon predicted that the …
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Redefining the Coordination of Gene Expression Machineries in Bacillus subtilis
… of bacterial gene expression. As soon as the ribosome binding site emerges from the RNAP, the ribosome can initiate translation, and both physically associate and kinetically coordinate with the RNAP over the course of transcription. This close proximity between the RNAP and ribosome allows …
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Structural studies of trans-translation
Ribosomes translate messenger RNA (mRNA) into protein in all living cells. The faultless production of protein is critical for a vast array of catalytic and structural roles and is essential for the survival of the cell. Ribosomes themselves are made up of both RNA and protein, and are composed of …
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Characterization of pigment production by Escherichia Coli containing a cloned Rhodococcus gene
… production in E. coli. This gene had a putative ribosome binding site and coded for an enzyme of Mr 42,560. Deletion analysis and in vitro transcription-translation experiments supported the hypothesis that pigment production in E. coli was due to a single enzyme. The gene and gene product did …
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RNA tools for optimization of multi-protein genetic systems
… sequences that can be inserted upstream of a ribosome-binding site to generate up to 175-fold repression when maximally expressed. By controlling sRNA expression, the targeted gene can be tunably repressed from 1.6- to 121-fold. We then built a pool where each of the six sRNAs was placed under …
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Understanding and stimulating cellular resource transactions for robust cell growth and genetic circuit performance
… to ODE's by simply adjusting the effective ribosome binding strength (RBS) of each genetic circuit is discussed. It is envisioned that a centralized controller which regulates resource production can be implemented to ensure robust circuit performance. The second half of this thesis …
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ROLE OF CRC IN THE REGULATION OF ALGINATE IN PSEUDOMONAS AERUGINOSA
… sigma factor, anti-sigmas and several DNA-binding transcriptional regulators. Here we examined the possibility that the catabolic repression control (Crc) protein repressor may affect alginate gene expression. A putative Crc binding site was observed adjacent to the ribosome binding site of …
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Characterisation and secretion mechanism of Bordetella pertussis autotransporter proteins
… other similar regions such as the RGD (integrin-binding) and SGXG (glycosaminoglycan-binding) motifs. The bap-5 gene also exists in B. bronchiseptica and B. parapertussis. Characteristic upstream regulatory sequences such as a ribosome-binding site were not seen in bap-5, but a potential …
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Mechanisms of cytokinin action
… activity. 2. Kinetin increases the turnover of ribosomes in floated Chinese cabbage leaf tissue. 3. The synthetic cytokinins kinetin and 6-benzylaminopurine bind reversibly to purifies Chinese cabbage leaf ribosomes. At 4°C, 0.8 molecules of kinetin and 1.08 molecules of 6BA are bound per …
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Understanding small RNA-mediated post-transcriptional regulation of Salmonella pathogenicity island 1 activator HilA in Salmonella typhimurium
… and Stnc3630, the results also defined sRNA InvR binding site within the hilA ribosome binding site region. Further genetic and molecular characterization showed that InvR directly base-pairs with hilA mRNA to prevent translation initiation, functioning to counteract HilA overactivation by HilD. …
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Dynamics of E. coli genome and cytosol under antibiotics
… of translation, and ”reversibility” of ribosome-binding antibiotics. In addition, we discover a strong correlation between the timing of responses from promoters in the Ori3 and Ter3 positions, and a small but consistent difference in the response magnitude between the two positions.
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Microbial synthesis of antimalarial compound FR-900098: pathway characterization and engineering
… the FR-900098 biosynthetic pathway through ribosome binding site (rbs) engineering.
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