Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 23 for “"ribosomally synthesized and post-translationally modified peptides (RiPPs)"”.
-
Mechanistic interrogations of ribosomal and non-ribosomal natural product enzymes
… herein is my thesis work towards structural and biochemical characterization of bacterial proteins involved in metabolite regulation and natural product biosynthesis. In the first case, I will describe the structural basis for transcriptional regulation of a small molecule metabolite, …
-
Characterization of Cyclopropyl Synthases Involved in the Maturation of Ribosomally Synthesized and Posttranslationally Modified Peptides
<p>Ribosomally synthesized and post-translationally modified peptides (RiPPs) are a large class of natural products with significant human health implications. RiPPs are synthesized from a genetically encoded precursor peptide that undergoes significant modifications by maturing enzymes, or …
-
On the biosynthesis and discovery of ribosomally synthesized and post-translationally modified peptides
Thiazole/oxazole-modified microcins (TOMMs) comprise a family of ribosomally synthesized and post-translationally modified peptides (RiPPs) that all contain thiazole and oxazole heterocycles derived from cysteine, serine, and threonine residues, respectively. The thiazole/oxazole heterocycle is …
-
Functional and mechanistic elucidation of peptide backbone thioamidation
… the ATP-dependent peptide backbone activation and subsequent formation of azoline heterocycles. It has been proposed that YcaO enzymes can also install lactamidines and thioamides onto peptides through similar mechanisms. These modifications are found in a class of natural products named …
-
Biochemical characterization of enzymes involved in the biosynthesis of the thiopeptide thiomuracin
… agents with novel modes of action. Thiopeptides are a family of ribosomally synthesized and post-translationally modified peptides (RiPPs) that have outstanding biological profile, including high potency against a variety of antibiotic resistant pathogenic strains. Their new modes of …
-
Expanding the structural diversity of the RiPP class of natural products
Ribosomally synthesized and post-translationally modified peptides (RiPPs) are a diverse and rapidly expanding class of natural products. Despite starting out as linear chains of amino acids, RiPP natural products acquire structural diversity ranging from small molecules like microcin C7 to 48-mer …
-
Design of Post-Translationally Modified Peptides by Combining Enzymes from Diverse Pathways
Over the past decade, ribosomally-synthesized and post-translationally modified peptides (RiPPs) have emerged as both therapeutically-relevant and engineerable, two traits previously unobserved together in a natural product class. Their biosynthesis is modular: a precursor peptide recruits enzymes …
-
Engineering of the microviridin post-translational modification enzymes for the production of synthetic protease inhibitors
Natural products and their derivatives have always been a source of drug leads. In particular, bacterial compounds have played an important role in drug development, for example in the field of antibiotics. A decrease in the discovery of novel leads from natural sources and the hope of finding new …
-
Mechanistic and synthetic studies on the prochlorosin and cytolysin families of lanthipeptides
Peptides are an attractive class of therapeutics, occupying a niche between small molecules and biologics. Research in the van der Donk lab focuses on lanthipeptides, a class of ribosomally synthesized and post-translationally modified peptides (RiPPs) that commonly feature antibacterial activity …
-
Post-translational modifications catalyzed by RiPP and RiPP-adjacent enzymes
Ribosomally synthesized and post-translationally modified peptides (RiPPs) are, as their name suggests, constructed from ribosomal peptides. The scope of biosynthetic reactions utilized by Nature to transform these peptides into structurally fascinating natural products is astounding. Leveraging …
-
Investigations into the molecular basis of enzyme-catalyzed reactions in natural product biosynthesis
… metabolites are involved in central metabolism and are essential for the survival of the organism. On the other hand, secondary metabolites are produced by organisms for an ‘added advantage’ effect. These molecules may be responsible for signaling and communication, antimicrobial defense, or …
-
Structural and biochemical studies on tailoring enzymes involved in ribosomal peptides biosynthesis
… caused by pathogenic infections. Both scientists and pharmaceutical companies have been dedicated in developing antimicrobial compounds to combat the micro-enemies. Despite the efforts, the resistant strains keep emerging with the introduction of new therapeutic compounds, which implies an urgent …
-
Characterizing peptide-modifying enzyme activity in RiPP biosynthesis
Ribosomally synthesized and post-translationally modified peptides (RiPPs) use enzymatic chemistry to diversify simple peptide backbones into architecturally complex and functionally rich natural products. These dedicated peptide-modifying enzymes install heterocycles, macrocycles, acyl groups, …
-
Biosynthesis and engineering of lanthipeptide natural products for novel applications
… products have played prominent roles in science and medicine due to their diverse chemical scaffolds and biological activities. Ribosomally synthesized and post-translationally modified peptides (RiPPs) have recently been recognized as a major class of natural products as a result of the genome …
-
Biosynthetic and bioinformatic investigation of RiPPs with radical SAM-installed crosslinks
Ribosomally synthesized and post translationally modified peptides (RiPPs) are an expanding class of natural products containing new chemical modalities and bioactive molecules. Although with diverse biological functions, significant research has been focused on investigating RiPPs with …
-
Investigation and application of substrate tolerant thioether forming enzymes
… biological functions in both prokaryotic and eukaryotic systems. Nature has evolved a treasure box of intriguing thioether containing natural products. Lanthipeptides contain thioether rings and have demonstrated diverse biological activities. This group of natural products are of great …
-
Biochemical and structural studies on proteins involved in the production of natural products and degradation of polysaccharides
… a starting material with both stereoselectivity and regioselectivity to produce a single product. Nature has solved this problem by evolving enzymes that specifically bind ligands and orientate them for selective modification. Furthermore, enzymes are generally active under mild conditions, …
-
Metabolite biosynthetic enzymes: catalysis, substrate specificity and protein engineering
… primary metabolites are involved in growth and development while secondary metabolites can function as signaling molecules, scavengers or chemical weapons. These small molecules have a myriad of diverse chemical structures that grant them biomedical relevance; however, their biosynthetic …
-
Investigating and engineering the post-translational modifications in RiPP natural product biosynthesis (I) Thiopeptides (II) Sactipeptides
… improved sanitation, effective vaccines, and antibiotics, bacterial infections remain a serious threat to human health. Annually, over 17 million people succumb to bacterial infections, with an increasing proportion due to antibiotic resistance. Therefore, there is urgent and continuous …
-
Exploring and understanding lantibiotic biosynthesis
… translates genomic information into structural and catalytic protein molecules. In addition to its role in protein synthesis, the ribosome also produces small non-catalytic peptides that exert antimicrobial properties. The recent surge in the availability of genome sequences from a wide variety …
Page 1 of 2