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Showing 1 to 20 of 82 for “"ribosomal protein"”.
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Transcriptional Regulatory Mechanisms of Ribosomal Protein Genes
Ribosomal protein genes are crucial for ribosome biogenesis. The ribosome itself is responsible for protein synthesis and hence cellular growth and development. Intertwining network of proteins in conjugation with cellular environment such as nutrition and growth factors collectively regulate …
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Isolation And Characterization Of Human Ribosomal Protein Complementary-dnas
… In Escherichia coli the isolation and cloning of ribosomal RNA genes and ribosomal protein genes was a prerequisite to detailed investigations of expression of these genes. Research into ribosome biosynthesis in eukaryotes has lagged behind in part because of the lack of data on the structure of …
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N-TERMINAL PROCESSING OF RIBOSOMAL PROTEIN L27 IN STAPHYLOCOCCUS AUREUS
… to cell growth yet little is known about how its proteins attain their mature structures. Recent studies indicate that certain Staphlyococcus aureus bacteriophage protein sequences contain specific sites that may be cleaved by a non-bacteriophage enzyme (Poliakov et al. 2008). The phage cleavage …
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A Probe Into Escherichia Coli Ribosomal Protein Topography Using Pronase
Made available in DSpace on 2015-05-14T14:58:49Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 7511542.PDF: 4582358 bytes, checksum: 1a07f54b1911962035b01e235e2f8038 (MD5) Previous issue date: 1974
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Ribosomal protein mutants and their effects on plant growth and development
… containing an RNA catalytic core, drive protein synthesis in all living organisms. 80S cytoplasmic eukaryotic ribosomes are comprised of four rRNAs and approximately 80 ribosomal proteins (r-proteins). R-proteins are encoded by gene families with large families (average of twelve members) …
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The evolution of ribosomal protein paralogues and their roles in development
All cellular protein synthesis - which is indispensable for cell growth, proliferation, and viability - is carried out by ribosomes. The production of sufficient functional ribosomes is essential for development and homeostasis, and reductions in the abundance of functional ribosomes can lead to …
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The role of a highly conserved eubacterial ribosomal protein in translation quality control
… the most crucial determinant of the quality of protein synthesis. Ribosomal protein L9 was first implicated in decoding fidelity when a mutant version of L9 was found to increase the translation of a T4 phage gene. Later studies confirmed that the absence of L9 leads to increased translational …
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Genetic Mapping of 30s and 50s Ribosomal Protein Cistrons by Intergenerictransduction and Conjugation
Made available in DSpace on 2014-12-11T18:16:49Z (GMT). No. of bitstreams: 1 7114898.pdf: 3686397 bytes, checksum: 89e77b44f033966941ac24bb0730bea7 (MD5) Previous issue date: 1970
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Functions of the eukaryote-specific ribosomal protein Asc1 /RACK1 in gene-specific translational activity
… single molecular entity, it is composed of both ribosomal RNAs and dozens of proteins. However, the individual contributions of most ribosomal components to translational regulation are unknown. In Chapter 1, I will review the current state of knowledge related to the functions of ribosomal …
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Assembly of the 30S ribosomal subunit: positioning of ribosomal protein S13 in the S7 assembly branch
<p>Studies of Escherichia coli 30S ribosomal subunit assembly have revealed a hierarchical and cooperative association of ribosomal proteins with 16S ribosomal RNA; these results have been used to compile an in vitro 30S subunit assembly map. In single protein addition and omission studies, …
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Structure determination of a yeast ribosomal protein L30 and pre-mRNA binding site complex by NMR spectroscopy
The yeast (Saccharomyces cerevisiae) ribosomal protein L30 and its auto-regulatory pre-mRNA binding site provide one of the best examples the critical role of protein-RNA interactions in regulation of RNA processing and control of gene translation. A model system for this interaction, which …
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Characterization of an operon containing a ribosomal protein gene and lipid biosynthetic genes in Escherichia coli K-12
… relative to the plsX gene. Each ORF encoded a protein, as demonstrated by radiolabeling in maxicells. ORF1 (orfY) encodes a protein of unknown function. ORF2 and ORF3 (rpmF) were sequenced prior to this study and encode a protein called G30k of unknown function and L32, a protein of the large …
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The L30e ribosomal protein from S. cerevisiae binds to its transcript RNA more strongly than to its primary target helix 58 of ribosomal RNA
<p>In <em>S. cerevisiae</em>, ribosomal protein L30e, in addition to being an integral part of the ribosome, autoregulates its levels of expression by: 1) binding to its transcript, inhibiting splicing; and 2) to its messenger RNA, inhibiting translation. This work explores ribosomal protein L30e …
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Effect of heat stress of the cell wall of the yeast, Saccharomyces cerevisiae : phosphorylation of ribosomal protein S10-B brought about by enzymes of the glycolytic pathways
The cell wall is a dynamic and elastic structure that provides osmotic and physical protection to the yeast cell (l). As such, it forms the immediate site of contact between the yeast cell and its environment (2), and is essential for maintaining cell integrity and shape. Cell growth and …
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The Further Characterization of <i>Saccharomyces cerevisiae</i> Ribosomal Protein L30 and Its Interaction with the <i>RPL30</i> Transcript through Mutational Analysis, Circular Dichroism, and Fluorescence Methods
<p>The <em>Saccharomyces cerevisiae</em> ribosomal protein L30 is one of a unique group of proteins that bind to their own mRNA in a feedback regulation mechanism. L30 binds to a stem-loop-stem region in the <em>RPL30</em> pre-mRNA which is called the kink-turn motif. This motif is found in several …
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X-Ray crystal structure of a ribosomal protein S6, S15, S18 : rRNA complex from T. thermophilus, and investigation of conformational changes in 16S rRNA fragments from B. stearothermophilus during ribonucleoprotein assembly
… structure of a fragment of the 30S ribosomal subunit from Thermus thermophilus containing ribosomal proteins S6, S15, S18, and a minimal rRNA binding site (T4 RNP) containing two different three-helix junctions was solved to 2.6 A. The protein S15 contains four bundled a-helices and …
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Endonuclease processing in the rif region of the Escherichia coli chromosome
… contains the secE-nusG and the rplKAJL-rpoBC (ribosomal protein) operons and it is thought that endonuclease processing by RNase E and RNase III plays some role in translational regulation and/or degradation of these transcripts. The purpose of this study was to investigate the effect that …
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Analisi Molecolare del Melanoma
… comparative proteomic analysis revealed that six proteins were overexpressed in both melanoma cell lines as compared with melanocytes: galectin-1, inosine-5'-monophosphate dehydrogenase 2, serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A ¿ isoform, protein DJ-1, cyclophilin A …
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Characterization of Transcriptional Components in Methanocaldococcus Jannaschii: An Integrative Study of Archaeal Basal Transcription Factors and Putative Cis Elements
… we uncovered conserved features upstream of ribosomal protein genes, some of which carrying transcription-/translation-regulatory functions.
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