Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"ribosomal RNA (rRNA)"”.
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SCREENING FOR POTENTIAL ANTIBACTERIAL AGENTS AND THE APPLICATION OF ISOTOPIC LABELS FOR THE CHARACTERIZATION OF THEIR EFFECTS ON THE DYNAMICS OF THE BACTERIAL 16S RIBOSOMAL RNA
… (nt) construct of the bacterial 16S ribosomal RNA (rRNA). We show that, at high concentration, these compounds interfere with bacterial growth. We also observed that the use of efflux pump inhibitors in combination with the fragments enhanced their antibacterial properties and may …
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Investigations into Why Human (but not mouse) KU70/80 is Essential Reveal a Role for Human KU70/80 in Ribosome Biogenesis
… the nucleolus, human Ku70/80 null cells exhibit ribosomal RNA (rRNA) processing defect and consequent shutdown of protein synthesis offer a direct explanation for cell viability loss. Through a series of complementary experiments, we also show that it is human Ku80that is required for Ku70/80 …
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Investigating WDR12 as a Therapeutic Target in Breast Cancer
… cells. We show that WDR12 knockdown induces ribosomal RNA (rRNA) processing defects resulting in strong growth inhibition in all breast cancer cells tested, however only a subset of lines displayed short-term cell cycle defects. To further understand the cellular consequences of WDR12 …
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MICROBIAL COMMUNITY ASSEMBLY AND DIVERSIFICATION OF THE GENUS CHLOROFLEXUS ALONG AN ALKALINE HOT SPRING GRADIENT
… of non-random assembly, as only one major 16S ribosomal RNA (rRNA) sequence was detected. Therefore, I next explored whether this single 16S rRNA sequence represents a single, broadly-distributed generalist or several cryptic specialist lineages. I isolated eleven strains of Chloroflexus from …
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MICROBIAL COMMUNITY ASSEMBLY AND DIVERSIFICATION OF THE GENUS CHLOROFLEXUS ALONG AN ALKALINE HOT SPRING GRADIENT
… of non-random assembly, as only one major 16S ribosomal RNA (rRNA) sequence was detected. Therefore, I next explored whether this single 16S rRNA sequence represents a single, broadly-distributed generalist or several cryptic specialist lineages. I isolated eleven strains of Chloroflexus from …
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Exploring conformational variability of an rna domain in the ribosome: from structure and function to potential antibiotic targeting
<p>RNA in nature is modified at many specific sites to order to gain extra functions or to expand the genetic code. One of such RNAs is ribosomal RNA (rRNA), which contains several modified bases, particularly around the functionally significant sites. We have focused on understanding the …
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Dna Aptamers Selected Against Wild-Type Helix 69 Ribosomal Rna And Their Implications In Combating Antibiotic Resistance
… is located in domain IV of Escherichia coli 23S ribosomal RNA (rRNA), undergoes conformational changes when in close proximity to the decoding region of 16S rRNA and transfer RNAs (tRNAs) in the peptidyl-transferase center (PTC). Functionally, the exact biological role(s) of H69 remains unclear; …
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A nucleic acid homology study of the genus Azospirillum
… is emended to accommodate A. amazonense. Ribosomal RNA (rRNA) homology studies indicated 90 to 96% homology between Azospirillum lipoferum and Azospirillum brasilense, and 64 to 70% homology between these species and Azospirillum amazonense. This supports the inclusion of these three …
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Synthesis, Characterization, And Rna Reactivity Of Amino-Acid-Linked Cisplatin Analogues
<p>The essential role of ribosomal RNA (rRNA) in the cell life cycle is highlighted by protein synthesis; therefore, the ribosome is considered to be an ideal drug target. Ribosomal RNAs exhibit a high level of structural diversity. The well-known anticancer drug cisplatin was previously applied as …
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Mechanism and regulation of ribosomal RNA transcription
The sole responsibility of RNA polymerase I (pol I) is the transcription of the ribosomal RNA (rRNA) genes. This transcription can account for up to 60% of the total cellular RNA that is being transcribed in an actively dividing cell. Due to the large amounts of energy this transcription requires, …
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Structural Studies of the Atypical Rio2 Kinase and N-Acetylglutamate Synthase
Rio2 is required for small subunit ribosomal RNA (rRNA) maturation. It is specifically involved in site D cleavage of the 20S rRNA to produce the mature 18S rRNA. Loss of Rio2p is lethal and a decrease of Rio2p activity results in 20S accumulation in the cytoplasm. One of the goals of this thesis …
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The Microbiome of Cystic Fibrosis Sputum and its Association with Incident Lower Airway Infections
… composition was assessed using V3-V4 16S ribosomal RNA (rRNA) gene sequencing. Relative abundance data were verified using both pathogen-specific qPCR and total 16S rRNA-targeted qPCR to assess bacterial bioburden. Strains were typed using pulse-field gel electrophoresis to differentiate …
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The origin and evolution of the ribosome
… the structural elements of the ribosome, namely ribosomal RNA (rRNA) and ribosomal proteins (r-proteins) provided a deep and the most comprehensive insight possible yet into the origins and evolution of the ribosome. Using phylogenetic methods that reconstruct the evolutionary history of complex …
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Characterizing Modified Nucleosides in RNA by LC/UV/MS
… in all types of ribonucleic acids (RNA) and increases the functional diversity of RNA, exceeding that of the four canonical bases. In ribosomal RNA (rRNA) and transfer RNA (tRNA) these modifications enhance RNA structure and contribute to the accuracy and efficiency of protein …
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Filamentous Foaming in Activated Sludge Systems: A Study Combining Molecular and Engineering Approaches
… in activated sludge. In contrast, the use of ribosomal RNA (rRNA)-targeted oligonucleotide hybridization probes allows for the reliable identification and quantification of specific microbial populations. Group-, genus-, and species-specific small subunit rRNA-targeted oligonucleotide probes …
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Purification and characterization of TFIIIA from Acanthamoeba castellanii
… interest has been in studying the mechanisms of RNA polymerase III (Pol III) transcription and the regulation of this process in A. castellanii, which was specifically focused on TFIIIA. This factor only activates Pol Ill-mediated transcription from 5S RNA genes and is the first of three general …
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Phenotypic and transcriptomic consequences of ribosomal DNA copy number variation in Caenorhabditis elegans and Saccharomyces cerevisiae
Ribosomal DNA (rDNA) forms the template for mature ribosomal RNA (rRNA) and shows considerable sequence conservation through evolution. In eukaryotic genomes rDNA is present in one or more tandem repeat arrays, with copy number variation (CNV) commonly observed in inter- and intra-species …
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The association of early childhood nutrition and growth with the intestinal microbiome and pneumonia
… young child feeding indicators. Microbiome 16S ribosomal RNA (rRNA) gene amplicon sequencing was performed and bioinformatic analysis conducted in QIIME2 and Phyloseq (R packages). Results Subsets of children in the DCHS were analysed. Growth analysis among 792 infants (50% female; 15% preterm) …
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rRNA Promoters as Targets for Transcription Factors: Structural and Functional Studies of PhERI and CarD
… of copying information encoded in DNA into RNA, to facilitate the expression of encoded proteins, is a central process in all living organisms. The expression and repression of subsets of genes allows different cell types in an organism to maintain diverse physiological roles and permits …
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Genomic tools to investigate and characterise emerging and reemerging disease in aquatic invertebrates
… sequencing methods to generate full ribosomal RNA (rRNA) arrays for key microeukaryotic pathogens of aquatic invertebrates, I improved the taxonomic resolution and enabled the design of robust diagnostic assays for closely related protist species. These methods were then applied to …
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