Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"ribonucleoprotein complexes"”.
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Precise Temporal Regulation of Ribonucleoprotein Complexes during the Drosophila Maternal-to-Zygotic Transition
… melanogaster embryo, the Smaug (SMG) RBP forms ribonucleoprotein complexes with corepressors, Trailer hitch (TRAL), Maternal expression at 31B (ME31B) and Cup, as well as AGO1 and the CCR4-NOT deadenylase to regulate the translation and stability of hundreds of maternal mRNAs, and this …
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Fantastic ribonucleoprotein complexes and how to study them. Architectural principles of regulatory assemblies in bacteria.
… to Crc and triggers assembly of higher order complexes. The structures suggest that secondary structure elements and the sequence of the target RNA determine the quaternary structure of the Hfq-Crc translation-repression assembly, introducing a degree of structural diversity that is …
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Identifying regulators of cytotoxic T cell function through molecular and genetic screening
… was successfully optimised in CTL using Cas9-ribonucleoprotein complexes resulting in efficient CRISPR-mediated loss of target proteins. Genes encoding known mediators of CTL cytotoxicity, $\it{Rab27a}$, $\it{Munc13-4}$ and $\it{Prf1}$, were targeted using CRISPR. The resulting samples were …
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Chemical Biology, Biochemical and Structural Studies of MDN1, an AAA Protein Required for Ribosome Biogenesis
… by ribosomes, which are ~3 MDa macromolecular complexes comprised of four ribosomal RNAs and ~80 ribosomal proteins in yeast. The biogenesis of such complicated ribonucleoprotein complexes is a highly regulated, multistep process requiring a plethora of more than 200 unique assembly factors. …
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Study Of Protein-Rna Interactions Using Fluorescence Resonance Energy Transfer (fret) And Single-Molecule Fret
<p>In the cell, RNA and protein, interact to form ribonucleoprotein complexes (RNPs) that have vital structural, catalytic and regulatory roles. Despite their functional importance, the mechanistic details and dynamics of RNPs are poorly understood. Single-molecule Fluorescence Resonance Energy …
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Investigating the mechanism of RNA packaging in Rift Valley Fever phlebovirus
… envelope glycoproteins, Gn and Gc, along with ribonucleoprotein complexes (RNPs), composed of encapsidated genomes carrying the nucleocapsid protein, N, and the RNA-dependent RNA polymerase, L protein. Understanding the mechanisms that govern the recognition and recruitment of viral RNPs for …
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The role of a toxin-antitoxin system in the arms race between bacteria and phage
… TifA is an RNA-binding protein that forms ribonucleoprotein complexes with ToxN, suggesting that TifA may sequester RNA-bound ToxN to prevent cleavage of phage transcripts during infection. Taken together, these results reveal the native targets and activation mechanism of an inducible, …
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Nanopore-based Protein Sensing with DNA Nanostructures
… first need to benchmark the specificity of the ribonucleoprotein complexes (RNPs) – something we can do using nanopores and DNA nanotechnology. We can use the combination of nanopores and DNA nanotechnology to investigate the utility of the RNPs not only for differentiating single nucleotide …
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Mechanisms and Evolution of Mitoribosomal Small Subunit Biogenesis
… The ribosome is made up of two conserved ribonucleoprotein complexes: the large subunit, responsible for peptide bond synthesis, and the small subunit, responsible for interrogating the match between mRNA codon and tRNA anti-codon. The assembly of the ribosome is a complex process which is …
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Novel RNA Targets of the Spinal Muscular Atrophy Protein
Ribonucleoprotein complexes (RNPs) are involved in many essential cellular processes, of which the most prominent examples include the ribosome that functions in protein translation and the spliceosome which catalyzes pre-mRNA splicing. The biogenesis of RNPs often involves complex and elaborate …
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Structural studies of spliceosome assembly and catalysis
… mRNA (pre-mRNA) from its component small nuclear ribonucleoproteins (snRNPs) and protein factors. A series of conformational and compositional changes produces the catalytically active spliceosome, in which the snRNA components of the snRNPs mediate intron recognition and catalysis of splicing. …
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eL15 and eL22 proteins and their functional environments during ribosome assembly in Saccharomyces cerevisiae
Ribosomes are ribonucleoprotein complexes in charge of protein translation. Translation is an essential process in all organisms that involves decoding the information contained in mRNAs (messenger RNAs) to generate proteins. Similarly, the process of ribosome biogenesis represents a critical …
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Molecular mechanisms behind the adjustment of phototrophic light-harvesting and mixotrophic utilization of cellulosic carbon sources in Chlamydomonas reinhardtii
… into translational silent messenger ribonucleoprotein complexes (mRNPs). The overexpression of NAB1 decreases LHCII protein amount whereas NAB1 knockout leads to an increased level of LHCII proteins. Consequently, NAB1 is part of a control system regulating the size and composition of …
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Biogenesis and Function of the Mitochondrial Ribosome
… ribosome (mitoribosome) is one of the largest ribonucleoprotein complexes in the cell, with the overall molecular mass of the fully assembled 55S monosome being ~2.7 MDa. It is indispensable for cell survival, as it translates 13 polypeptides encoded in mitochondrial DNA, which are essential …
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A Census of Human RNA-Binding Proteins and Characterization of the DEDDh RNA Exonuclease NEF-sp in the 3' End Maturation of 28S Ribosomal RNA
… RNAs, regulating and executing their maturation, ribonucleoprotein assembly, transport, stability and translation. RNA-binding proteins (RBPs) and ribonucleoprotein (RNP) complexes coordinate these processes. RBPs are central to cellular metabolism and their role in human diseases has been widely …