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Showing 1 to 20 of 88 for “"ribonuclease"”.
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Ribonuclease H2, RNA:DNA hybrids and innate immunity
… define the role of one of the AGS nucleases, the Ribonuclease H2 (RNase H2) complex, in innate immunity, and to establish if nucleic acid substrates of this enzyme were able to induce type I interferon production in vitro. The AGS nucleases may function as components of the innate immune response …
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Studies of the Thermal Denaturation of Ribonuclease
Made available in DSpace on 2014-12-05T19:38:02Z (GMT). No. of bitstreams: 1 6206160.pdf: 4358934 bytes, checksum: 466a2edb6e041df2725196e46e996a05 (MD5) Previous issue date: 1962
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Biological Functions of ribonuclease 1 and angiogenin
Pancreatic-type ribonucleases (ptRNases) are a large family of vertebrate-specific secretory endoribonucleases. They catalyze degradation of many RNA substrates, mediating a variety of biological functions. The homology shared by ptRNases has enabled extensive biochemical characterization and …
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Thermal adaptation of conformational dynamics in ribonuclease H
… relationship between the two techniques. Ribonuclease HI (RNase H), an 18 kD globular protein that hydrolyzes the RNA strand of RNA:DNA hybrid substrates, has been extensively studied by NMR to characterize the differences in dynamics between homologs from the mesophilic organism …
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Biochemical Studies of Ribonuclease P of Tetrahymena Thermophila
Photochemical crosslinking experiments with a photoaffinity-labeled substrate have been performed with the RNase P RNA subunit and holoenzymes from various sources. Experimental studies clearly reveal that the prokaryotic RNA subunit is involved in substrate binding, but that the eukaryotic RNA …
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Structure and Function of Colicin E5 Ribonuclease Domain
The binding of ImmE5 to ColE5 resulted in a 50% increase of fluorescence emissions. The isothermal titration calorimetry studies showed a -10 kcal/mol enthalpy change upon the formation of ColE5•ImmE5 complex. The mutational studies of ImmE5 indicated that ImmE5 K4 and D95 contributes a larger …
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Transfer Ribonucleic Acid and Ribonuclease Activity in Germinating Wheat Embryos
Made available in DSpace on 2015-05-13T22:12:02Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 6808257.PDF: 4171221 bytes, checksum: cd2bea80b8de8b190dadb7914446dce3 (MD5) Previous issue date: 1967
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Exploring and Exploiting Ribonuclease 1: from Protein Biochemistry to Protein Engineering
Ribonuclease (RNase) 1 is a human protein with a remarkable ability to indiscriminately hydrolyze RNA. RNase 1 and its bovine homologue RNase A exhibit ubiquitous expression across tissues, a catalytic efficiency within the diffusion-limited regime, and minimal substrate sequence requirements. …
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Regulation of escherichia coli ribonuclease III by the bacteriophage T7 protein kinase
Ribonuclease III (RNase III) is a double-stranded-RNA-specific nuclease of Escherichia coli. RNase III functions as a homodimer. The 25.6 kDa subunit contains two domains, a 148 residue catalytic domain and a 78 residue double-stranded-RNA-binding domain (dsRBD). RNase III is involved in the …
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Degradation of 23S rRNA in Azithromycin-Treated Ribonuclease Mutants of <em>Escherichia coli</em>.
… particle. When exposed to azithromycin, several ribonucleases in wild-type <em>Escherichia coli</em> cells degrade antibiotic-bound 50S precursor particles. Presumably, cells expressing one or more mutated ribonucleases will degrade the antibiotic-bound precursor less efficiently, resulting in …
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Interaction of Cu(II) ion with ribonuclease A in the native and unfolded states
<p>The binding of Cu(II) ion to ribonuclease A at various sodium perchlorate concentrations was studied as a function of Cu(II) ion concentration and pH using a Cu(II) ion electrode. Spectrophotometric studies of the same systems were also carried out. The copper binding studies were carried out at …
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Information Content in Ribonuclease A: An Investigation of Protein Structure and Identification of New Qsars
… model was developed for this quantification. Ribonuclease A (RNase A) was used as a model system of investigation.</p> <p>In solution, molecules are subjected to numerous collisions powered by the thermal energy of Brownian motion. It is upon each Brownian collision that structural information …
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