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Showing 1 to 20 of 22 for “"revertant"”.
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Natural and Exogenous Genome Editing In Wiskott-Aldrich Syndrome Patient Cells
… with WAS exhibit a phenomenon called Somatic Revertant Mosaicism which is characterized by the presence of lymphocytes which naturally revert back to normal phenotype by restoring WASp expression. To date, the mechanisms of this naturally-occurring gene therapy remains poorly understood, and …
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Molecular, Genetic, and Agronomic Evaluation of Wp and Wp-M in Soybean
… of wp-m when crossed to pink and purple flowered revertant lines, and (5) report the effect of wp upon agronomic traits in stable flowered lines derived from wp-m. The cloned 3'5 ' flavonoid hydroxylase enzyme fragments from soybean did not show restriction fragment length polymorphism (RFLP) …
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Genetic Dissection of DH31 Signaling in Drosophila
… excision lines led to the identification of a revertant, Dh31rev, and two mutant alleles, Dh3101 and Dh3102. Subsequent analysis of these alleles indicated that revertant lines exhibited wild type responses to osmotic and starvation stress, whereas mutant lines were hypersensitive to …
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Characterization of activation tagged potato (Solanum tuberosum L.) mutants
… the activation tagging vector pSKI074, and nikku revertant plants. Mutant up exhibited a dwarf phenotype (plant height 42 cm vs. 73 cm in cv. Bintje), abundant axillary shoot growth (3.1 shoots/plant compared to 0.7 shoots/plant in cv. Bintje; in vitro plants), greater tuber yield, altered tuber …
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Phenotypic Characterization of Mutations Made to the Envelope Protein of West Nile Virus
… experimental conditions were shown to select for revertant or adaptive mutations. Collectively, the results from this study showed that G331A mutation induces a multifactorial attenuation of WNV and that an additional revertant mutation (H395Y) restores many, but not all, of the impacted …
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Mutational analysis of integrase and excisionase proteins in bacteriophage lambda site-specific recombination system
… defective Int mutants. A second-site revertant P243L/E218K and a pseudorevertant E175K were isolated and further characterized using genetic and biochemical methods. The results showed that the second-site substitution E218K selectively enhances Int core-binding affinity and acts as a …
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Studies of SV40-transformation and the Loss of Growth Factor Requirements
… in the standard density of anchorage assays. A revertant of SV40 transformed 3T3 cells was found to have regained a very strong dependence upon insulin. This dependence was not overcome by re-transformation with Kirsten Murine Sarcoma virus, although retransformation did obviate both its …
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Cytotoxicity and Mutagenicity of Disinfection Byproduct Mixtures Formulated Using Free Chlorine and Monochloramine
… by the identified DBPs. In the Ames tests, no revertant wells were observed with the TA-98 bacterial strain, indicating the DBP-concentrates did not induce frameshift mutations in hisD3052. However, statistically significant increases in the number of revertant wells were observed in TA-100 in …
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Ιστοπαθολογικές αλλοιώσεις σε ποντίκια μετά από ενδοπεριτοναϊκή χορήγηση της γλυκολιποπρωτεΐνης (G.L.P.) του Slime της pseudomonas aeruginosa
… and Bartell 1974) from a non mucoid strain and a revertant non mucoid variant of Pseudomonas aeruginosa. Alginate also was obtained from a mucoid strain of Pseudomonas aeruginosa. The histopathology of mice induce by a single intraperitoneal (ip) LD₅₀ dose of there substances was studied. Alginate …
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Regulation of Expression of a Retroviral Transforming Gene and its Cellular Homolog
… the isolation and characterization of phenotypic revertants of rat cells transformed by FSV. Analysis of the defect of the two ts mutants of FSV revealed that P130 is the transforming protein of FSV and that its kinase activity plays a crucial role in cell transformation by this virus. v-fps, the …
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Study of the roles of Cyprinid herpesvirus 3 ORF134 in the biology of the infection
… a strain deleted for ORF134 and a derived revertant strain were produced by using BAC cloning and prokaryotic recombination technologies. Comparison of these strains demonstrated that CyHV-3 ORF134 does not contribute significantly to viral growth in vitro or to virulence in vivo in the …
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Characterisation of open reading frames m29 and m29.1 in murine cytomegalovirus
… by in vitro passage in tissue culture cells. Revertant virus (Rv29.1) was made by a further 2 step process in which the mutant m29.1 ORF was first replaced by the antibiotic cassette and then by the wt ORF. These mutants were characterized both in tissue culture and in immunocompetent BALB/c …
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Molecular biology studies on thecyd operon of Escherichia coli
… cooperative. These models are confirmed by revertant analyses. Finally the cydC gene locus is required for the activity of the cytochrome d complex.
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ΣΥΜΒΟΛΗ ΣΤΗ ΜΕΛΕΤΗ ΚΑΙ ΤΟ ΧΑΡΑΚΤΗΡΙΣΜΟ ΤΗΣ ΕΞΩΚΥΤΤΑΡΙΑΣ ΟΥΣΙΑΣ (SLIME) ΒΑΚΤΗΡΙΑΙΜΙΚΟΥ ΒΛΕΝΝΩΔΟΥΣ ΚΑΙ ΘΥΓΑΤΡΙΚΟΥ ΜΗ ΒΛΕΝΝΩΔΟΥΣ ΣΤΕΛΕΧΟΥΣ PSEUDOMONAS AERUGINOSA
… HEPATITIS DUE TO ANTITUBERCULOUS THERAPY. A REVERTANT NON MUCOID VARIANT (RNMV) FROM THE PARENTAL MS WAS OBTAINED BY SERIAL PASSAGE ON TRYPTICASE SOY AGAR. THE EXTRACELLULAR MATERIAL (SLIME) FROM BOTH STRAINS WAS EXTRACTED BY METHODS AS FOR THE EXTRACTION OF GLYCOLIPOPROTEIN (GLP). GROSS …
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Role of Flagellar Motility in Apple Blossom Invasion and Tactic Response to Various Plant Nectar Extracts by Erwinia Amylovora
A nonmotile isolate (Nm22) and its motile revertant (MR22) of Erwinia amylovora were obtained after treating cells of the wild type (Isolate 110) with 0.3 M ethyl methanesulfonate. The pathogenicity of both isolates was similar with the wild type when inoculated to young shoots of apple seedlings. …
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The in planta role of the global regulator Lrp in the bacterial phytopathogen Pantoea stewartii subsp. stewartii
… the in-frame deletion of lrp, as well as a revertant strain. The strain with the deletion of the lrp gene showed reduced motility and capsule formation when in vitro assays were conducted. It has previously been demonstrated that these characteristics are both important for the bacteria's …
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The Multifunctional Capsid Gene of Hepatitis C Virus
… four stop codons was severely impaired, but the revertant genomes showed a marked improvement in replicative fitness. Consistent with the chimpanzee infection, reversions at stop codons 3 and 4 were also selected by passage of mutant, infectious HCV in cell culture. Genetic evidence for RNA …
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A Genetic and Physiological Study of the Role of Extracellular Copper-Binding Proteins in Copper Detoxification by the Marine Bacterium <i>Vibrio alginolyticus</i>
… CuBP. After fifteen nonselective subcultures, a revertant Cu<sup>s</sup> derivative of Cu40B3 (Cu40B3(SW)) was isolated. Cu40B3(SW) lost the mutation to constitutive CuBP production and copper resistance simultaneously, indicating that constitutive CuBP production in Cu40B3 is necessary for …
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Tumour evolution in ovarian cancer using high-throughput genomics technologies
… ,"genome and lost platinum sensitivity after a revertant BRCA2 mutation restored its HR function. A subset of tumours from the Cancer Genome Atlas (TCGA) dataset suggested that these two phenotypes were mutually exclusive. Amongst the six OV03 cases, preliminary analysis suggests that one case …
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Glycoprotein M and ESCRT in herpes simplex virus type 1 assembly
… cells, a HSV-1 gM deletion virus (∆gM), and its revertant virus were constructed. The major phenotype demonstrated was that the absence of gM caused the internalisation of cell surface gH/L to be inhibited and higher levels of gH/L to be observed on the cell surface. Further, lower levels of gH/L …
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