Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 12 of 12 for “"reversible binding"”.
-
Reversible binding of dihydrogen to dinuclear ruthenium complexes containing chelating diphosphines
… and the η²-H₂ ligand is replaceable by N₂. The reversible equilibrium conversion under H₂ of [RuCl(DPPB)₂(μ-Cl)]₂, 9, to (η²-H₂)Ru(DPPB)(μ-Cl)₃RuCl(DPPB), 9a , in CH₂Cl₂ has been studied at 0-25°C by UV-vis and ³¹P{¹H}NMR spectroscopy, and by stopped-flow kinetics; the equilibrium constant and …
-
Engineering streptavidin and its target ligands with both infinite binding affinity and reversible binding capability
… ideal for streptavidin to have extremely tight binding to its target ligands (biotin and its binding tags) and at the same time to retain the feature of reversible binding capability. To achieve this objective, a streptavidin mutein (SAVSBPM32) was engineered based on a previously engineered …
-
Interactions Between Heme-Globins and Ligands
… (O2 and NO), cellular oxygen homeostasis, and reversible binding of O2 with a higher binding affinity than hemoglobin.6 Tejero et al. previously showed that a mutant form of Ngb reduces nitrite to nitric oxide 50x faster than myoglobin and 500x faster than hemoglobin.7 Ngb also tightly binds to …
-
Towards Colloidal Self-Assembly for Functional Materials
… to the highly specific, tunable and thermally reversible binding between DNA strands. Gold nanoparticles coated with DNA were used to investigate the temperature-dependent interaction potentials and the gel formation in DNA-colloidal systems. The electronic conductivity and the plasmonic …
-
Estrogens Containing Alkylating Substituents: The Synthesis and Estrogen Receptor Interactions of Estradiol and Hexestrol Affinity Labels
… more reactive than the chloro ones. Their reversible binding to the lamb uterine estrogen receptor was measured by competitive binding assays, and their irreversible reaction with receptor was measured by exchange assays that determine the rate and extent of receptor inactivation. In …
-
Modeling and simulation of coronary stents: Intravascular drug delivery and arterial drug distribution
… model that couples drug diffusion and reversible binding in the arterial wall. The model is solved by the finite volume method with varied stent-coating and vascular diffusivities for (i) drug loading at concentrations less than or equal to the solubility, and (ii) high drug loading at …
-
Nanoparticle Self-Assembly for the Synthesis and Processing of Ordered Nanocomposite Solids
… through the use of dynamic supramolecular binding interactions. However, while dynamic interparticle interactions are key for enabling reversible binding and preventing kinetic traps during the assembly process, they render assembled structures susceptible to dissociation upon changes in …
-
Programmable, isothermal disassembly of DNA-linked colloidal particles
… This project focuses on using programmable and reversible binding between oligonucleotide strands to assemble and then disassemble polystyrene colloidal particles. It is shown that DNA-mediated assembly can be reversed at a fixed temperature using secondary oligonucleotide strands to …
-
Exploring The Ligand Binding Site On The Dopamine Transporter By Photoaffinity Labeling And Site-Directed Mutagenesis
… and inhibit its transport activity, but their binding site within the protein and the mechanism by which they block DA transport remain unclear. Two distinct ligand interaction sites at transmembrane domains (TMs) 1-2 and 4-6 have been identified using irreversible uptake-blockers. The current …
-
The relationship between cupric ion activity and the toxicity of copper to phytoplankton
… ion activity. The above relationship suggests a reversible binding of copper to a single set of cellular ligand sites having a total binding capacity of 4.8 x 10-16 moles per cell and an association constant for reaction with copper of 109.2. For T. pseudonana (clone 3H) copper was inhibitory at …
-
Mechanistic Study of the Adsorption and Desorption of Proteins on Silica
… perturbations on protein desorption, where irreversible conformational changes might lead to various forms of protein destabilization. We study the adsorption of lysozyme on silica and find that not only is adsorption reversible, but also that desorption is predictable in a coverage-dependent …
-
Structural Properties of α-Synuclein in Functional and Pathological Contexts
… in α-helix. Characterising the nature of this binding as well as the structural and functional properties of αS at the surface of biological membranes is currently a top challenge. In particular the intrinsic limitation of current analytical techniques in studying highly heterogeneous protein …