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Showing 1 to 18 of 18 for “"reversed-phase liquid chromatography"”.
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Supercritical Fluid Extraction Directly Coupled with Reversed Phase Liquid Chromatography for Quantitative Analysis of Analytes in Complex Matrices
… Fluid Extraction (SFE) with High Performance Reversed Phase Liquid Chromatography (HP-RPLC), and to explore its ability for quantitative analysis of analytes in different matrices. First, a simple interface was developed via a single one six-port injection valve to connect the SFE and LC …
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The characterization of silica-based sorbents in reversed phase liquid chromatography with application to method development in solid-phase extraction
Despite the wide application of solid-phase extraction (SPE) in the environmental, clinical and pharmaceutical fields, method development for this technique has remained largely an empirical process of trial and error. In an effort to provide a systematic approach to method development, the kinetic …
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Reversed-Phase Liquid Chromatography Tandem Mass Spectrometry And Elisa Determination Of Caffeine As A Marker For Sewage Contamination Of Wilgreen Lake
… and its tributaries, and analyzing them using liquid chromatography-tandem mass spectrometry coupled to an Electrospray Ionization (ESI) Interface. An isotope dilution method using 13C3-labeled caffeine as the internal standard was adapted from known methods and employed to determine and …
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Extraction of Additives from Polystyrene and Subsequent Analysis
… 1) the development of a high performance liquid chromatography (HPLC) method(s) for the additives and 2) the determination of the viability of supercritical fluid extraction (SFE) for the additives from polystyrene. Separation of some of the additives was achieved using reversed phase …
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The Removal of Pharmaceuticals from Wastewater by Wet-Air Oxidation
… drug concentrations were determined by reversed-phase liquid chromatography. The influences of different operational conditions on removal efficiency of the drugs by WAO were evaluated, namely reaction time, initial drug concentration, oxygen concentration, and the amount and composition …
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Feasibility of analysis of polar compounds by high performance liquid chromatography with Fourier transform infrared spectroscopic detection
High performance liquid chromatographic separations employing on-line flow cell Fourier transform infrared spectroscopic detection were developed for polar compounds including phenols, alcohols, amines and azaarenes. Detection by FTIR gave information concerning hydrogen bonding and solvent effects …
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Studies of UHPLC-MS performance and application to rapid sensitive and robust drug analysis
… comprises studies of ultra high performance liquid chromatography (UHPLC) performance with a particular focus on the application of liquid chromatography-mass spectrometry (LC-MS) for the analysis of basic drugs. UHPLC-MS technologies are investigated to facilitate fast, sensitive and robust …
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Fumonisins : chromatographic methodology and their role in human and animal health
… purification of crude extracts, derivatisation, reversed-phase liquid chromatography separation and fluorescence detection, was subjected to an international collaborative study involving 11 laboratories from 6 countries. Although the results established that the method was highly reproducible, …
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Mass Spectrometric Characterization of the MCF7 Cancer Cell Line: Proteome Profile and Cancer Biomarkers
… using a shotgun 2-D strong cationic exchange/reversed phase liquid chromatography electrospray ionization tandem mass spectrometry (SCX/RP-LC-ESI-MS/MS) protocol. A series of optimization strategies were performed to improve the LC-MS experimental set-up, sample preparation, data acquisition …
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A STUDY OF THE METAL-BINDING ORGANIC CONSTITUENTS IN GREAT BAY SEDIMENTARY SYSTEMS
… molecular weight greater than nominally 500 amu. Reversed-phase liquid chromatography results showed that organics in pore water were more polar than material in the other extracts. It was found that the deionized water removed more organic matter than seawater, but the latter removed organics …
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Geographic and Ontogenetic Variation of Venom in the Rattlesnakes Crotalus oreganus oreganus and Crotalus o. helleri
… In the first of three empirical studies, I used reversed-phase liquid chromatography and mass spectrometry to examine the venome of four C. o. helleri populations. Substantial geographic variation existed, with small basic peptides (myotoxic β-defensins) expressed in large amounts by all …
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Selectivity and temperature effects in high performance liquid chromatography
A study of six liquid chromatographic column packing materials was conducted. The materials investigated were: SI-60, SI-100, ALOX T, RP-2, RP-8, and RP-18 (E. M. Laboratories, Elmsford, N.Y.). The column packing materials had an average particle diameter of 10 µm and were slurry packed into …
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Arginase from Bacillus caldovelox
… and arginine decarboxylase activity by a reversed-phase liquid chromatography method developed to detect these arginine-catabolizing enzymes in cell-free extracts. Thermus, Bacillus and Sulfolobus strains produced arginase, but arginine deiminase and arginine decarboxylase activities were …
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Analysis of Nucleosides and Nucleotides in Milk and Infant Formula
… milk. A method utilising anion exchange solid-phase extraction clean-up and liquid chromatography was developed for the rapid, routine determination of supplemented cytidine 5′ monophosphate, uridine 5′ monophosphate, inosine 5′ monophosphate, guanosine 5′ monophosphate, and adenosine 5′ …
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Reversed-Phase HPLC Determination of Cholesterol in Food Items.
… is presented. This involves a C-18 stationary phase using a 70:30 methanol: 2-propanol mobile phase with an UV detector set at 212 nm. The method showed linearity in the range 5.0 to 100.0 μg/mL and also good reproducibility with relative standard deviation of 4.22%, 2.71%, 4.8%, and 3.7% for …
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Molecular Response of Retinal Pigment Epithelial Cells to Oxidized Lipoproteins: Global and Targeted Studies
… proteome fractionation platforms: polymeric reversed-phase liquid chromatography at high pH (PLRP), sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and isoelectric focusing (IEF) separations. We applied a liquid chromatography-mass spectrometry/mass spectrometry …
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Studies of Capsaicinoids Contents of Locally Grown and Commercial Chilies Using Reversed-Phase High Performance Liquid Chromatography.
… method involves 73:27% methanol water mobile phase with C<sub>18</sub> stationary phase and UV-Vis detector set at 210 nm. The method showed good reproducibility with 1.74% - 4.72% relative standard deviations, a linear response within 0.65–45.5 and 0.25-17.5 μg/mL for capsaicin and …