Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"reverse phase protein array"”.
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Evaluating The Therapeutic Efficacy of Grb2 Inhibition In Ovarian Malignancies
<p><strong>Purpose: </strong>Adaptor proteins such as growth factor receptor-bound protein-2 (Grb2) play important roles in cancer cell signaling. In the present study, we examined the biological effects of liposomal antisense oligodeoxynucleotide that blocks Grb2 expression (L-Grb2) in ovarian …
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Harnessing Cancer Omics to Inform Precision Oncology
… proteomics from kidney tumor samples using reverse phase protein array (RPPA) and formulated an MTOR score that improves prognosis prediction of recurrent risk given Leibovich risk scoring. Finally, we selected high quality user queries to finetune the LLM modules of DrBioRight, evaluated …
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Dual Assembly Nanoparticles Achieve Sustained Silencing of Znf304: A Novel Transcription Factor For Β1 Integrin
… survival in ovarian cancer patients. Through reverse phase protein array analysis, we demonstrated that ZNF304 promotes multiple proto-oncogenic pathways important for cell survival, migration, and invasion. ZNF304 transcriptionally regulates β1 integrin, which subsequently regulates Src/focal …
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Downregulation of Pax2 Suppresses Ovarian Cancer Cell Growth
… had slower growth and migration rates. Microarray gene expression profile analysis further identified genes that were affected by PAX2 including the tumor suppressor gene <em>G0S2</em>. Reverse phase protein array (RPPA) data showed that PAX2 knockdown affected several genes that are involved …
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Yap1 Expression Predicts Sensitivity to Dual Akt/P70S6K Inhibition In Ovarian and Uterine Malignancies
… Western blot analysis, plasmid transfection, and reverse phase protein array [RPPA]) were carried out to characterize underlying mechanisms and potential biomarkers of response.</p> <p><strong>Results: </strong>MSC2363318A decreased tumor growth and metastases in multiple murine orthotopic models …
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Inhibition of the endosomal recycling pathway to overcome resistance to cancer therapies
… to downregulate clinically relevant cell surface proteins and to overcome drug resistance. This thesis focuses on two clinically relevant hormone receptors that are strongly linked to the development and progression of breast and prostate cancer linked to disease development and progression, the …
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Glycogen Synthase Kinase 3 Is Required For Optimal Akt Activation
… a reduction in phosphorylated AKT. Analysis of reverse phase protein array screening of MDA-MB-231 breast cancer cells treated with RNA interference targeting GSK3 unexpectedly revealed an increase in levels of phosphorylated MAPK14 (p38). Treatment with the selective p38 inhibitor SB 202190 …
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ANTIBODY-DRUG CONJUGATES FOR THE TREATMENT OF BREAST CANCER.
… quantitative proteomic (High Sensitivity-HER2, Reverse Phase Protein Array), transcriptomic (HER2DX) and circulating tumor DNA (DNADX) assessment of HER2 on pre-T-DXd samples enabled a refined prediction of T-DXd efficacy, including in subgroup analyses of HER2-positive and HER2-negative breast …
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Modulation of Pi3K-Akt Pathway In Colorectal Cancer
… (PD) markers and signaling pathways using reverse phase protein array (RPPA) on (1) paired tumor biopsies from a clinical trial of MK2206 in PI3K-altered metastatic CRC, (2) PTEN<sup>loss</sup>CRC patient derived xenografts (PDX) treated with MK2206 or carrier, and (3) MK2206-treated cell …
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Investigating The Roles of Lung Epithelial Stat3 In Therapeutically Inducible Resistance to Acute Bacterial Pneumonia.
… underlying inducible resistance, we performed reverse phase protein array analysis and identified STAT3 phosphorylation as a potential mediator of synergy. There was a robust activation of epithelial STAT3 by Pam2ODN treatment and we demonstrated that it was independent of IL-6 signaling. While …
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Mathematical Modeling Reveals That G2/M Checkpoint Override
… we performed a multidisciplinary study combining reverse phase protein array (RPPA) data, molecular biology and mathematical modeling to systematically identify molecules required for DNA damage checkpoint recovery. The mTOR complex 1 (mTORC1) plays an essential role to regulate mitotic entry …
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Mathematical Modeling Reveals That G2/M Checkpoint Override Creates A Therapeutic Vulnerability In Tsc2-Null Tumors
… we performed a multidisciplinary study combining reverse phase protein array (RPPA) data, molecular biology and mathematical modeling to systematically identify molecules required for DNA damage checkpoint recovery. The mTOR complex 1 (mTORC1) plays an essential role to regulate mitotic entry …
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Mathematical Modeling Reveals That G2/M Checkpoint Override Creates A Therapeutic Vulnerability In Tsc2-Null Tumors
… we performed a multidisciplinary study combining reverse phase protein array (RPPA) data, molecular biology and mathematical modeling to systematically identify molecules required for DNA damage checkpoint recovery. The mTOR complex 1 (mTORC1) plays an essential role to regulate mitotic entry …
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Interactions Between Host Cells And Merkel Cell Polyomavirus In The Context Of Infection And In Merkel Cell Carcinoma
… a lead compound using cell viability assays, a reverse-phase protein array, and western blotting. This compound induces DNA damage and cell death specifically in MCPyV-positive MCCs. Combined treatment with BCL-2 inhibition led to more complete killing and expanded the phenotype to a broad set …
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Bst2 Isoforms: Distribution and Potential Use In Cancer Therapeutics
… is a raft-associated type II transmembrane protein with an unusual topology. Analyses showed a variable degree of BST2 expression in most organs. Two research groups identified different BST2 isoforms of BST2 generated by posttranscriptional modification. In this research, I studied the long …
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Altered Ovarian Cancer Metabolism Increases Neuronal N-Acetylaspartate to Promote Tumor Growth
… were evaluated for gene expression and protein expression of NAT8L, respectively. Gene expression of NAT8L and clinical outcomes were further investigated from publicly available databases from the cancer genomics atlas (TCGA) using www.cbioportal.org, and two previously published …
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Combined Inhibition of Ddr1 and Cdk4/6 Induces Synergistic Effects In Er-Positive, Her2-Negative Breast Cancer With Pik3Ca/Akt1 Mutations
… 3‑kinase (PI3K)/ serine/threonine protein kinase B (AKT) signaling pathway occur frequently in estrogen receptor–positive (ER-positive) breast tumors. Patients with ER-positive, human epidermal growth factor receptor 2–negative (HER2-negative) metastatic breast cancer are often …
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Identifying and Targeting Adaptive Changes to Bet Inhibition In Ovarian Cancer
… for all <em>in vitro</em> (MTT, apoptosis, protein expression, reverse phase protein array (RPPA) analysis and <em>in vivo</em> (orthotopic mouse model)) experiments. The poly (ADP-ribose) polymerase (PARP) inhibitor olaparib, as well as the mechanistic target of rapamycin (mTOR) inhibitors …
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Post-translationally Modified Glucocorticoid Receptors and Protein Tyrosine Kinase 6 Modulate Triple Negative Breast Cancer Phenotypes
… Growth Factor β1 (TGFβ1) and Mitogen Activated Protein Kinase (MAPK) signaling gene sets associated with TNBC cell survival and migration/invasion. Accordingly, pS134-GR was essential for TNBC cell anchorage-independent growth in soft-agar, migration, invasion, and tumorsphere formation, an in …
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The Role of Acidic Fibroblast Growth Factor and Fibroblast Growth Factor Receptor 4 In High Grade Serous Carcinoma
… correlation studies on data generated from array CGH and transcriptome profiling analyses on 51 microdissected tumor samples were used to identify genes located on chromosome 5q31-35.3 that showed significant correlation between DNA and mRNA copy numbers<strong>. </strong>Significant …
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