Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 85 for “"reverse genetics"”.
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The development of a reverse genetics system for Machupo virus
… and large segments, and the establishment of a reverse genetics system and rescue of a recombinant MACV. Using these tools, I present the first modern in vitro characterization of MACV, the development of a novel and lethal murine animal model, and the generation of a rationally attenuated MACV.
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The use of reverse genetics to investigate plant-parasite interactions
This thesis demonstrates the development and use of a novel Virus Induced Gene Silencing platform to investigate the interactions of plants and parasites. Host and parasite genes are targeted using this technology which when silenced indicate their involvement in establishing pathogenicity. The …
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A reverse genetics study of the Human Coronavirus 229E ADP-ribose-1"-phosphatase
… -1”-phosphatase) was investigated using a reverse genetics approach. To better understand the role of the ADRP in viral replication, a set of HCoV-229E ADRP mutants was generated, including viruses with a (i) mutation in the putative ADRP substrate-binding site (N1357A), (ii) deletion of …
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Analysis of Tribolium head patterning by forward and reverse genetics and transgenic techniques
In dieser Doktorarbeit wurden verschiedene Methoden angewandt um ein besseres Verständnis der Insektenkopfentwicklung zu erlangen. Als Modellorganismus wurde der Rotbraune Reismehlkäfer Tribolium castaneum ausgewählt. Dieser Organismus ist für die Analyse dieses Prozesses gut geeignet, da Larven …
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Reverse Genetics-based Approaches to Attenuate Porcine Reproductive and Respiratory Syndrome Virus (PRRSV)
… outbreaks. With the availability of the PRRSV reverse genetics systems, rapid vaccine development against PRRSV through the manipulation of the reverse genetics becomes feasible. To facilitate the vaccine development effort and study of PRRSV genes, we first established a DNA-launched …
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Reverse genetics analysis of biological functions of zinc transporters Znt1 (Slc30a1) & Zip10 (Slc39a10) in zebrafish
… species. In the present study, we have used genetics and molecular approaches in a model vertebrate system (the zebrafish Danio rerio) to elucidate the nutritional and functional importance of zinc and its transporters in the regulation of some crucial biological processes in the body. The …
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Reverse genetics system and fucntion of NSM protein of Rift Valley fever virus (family Bunyaviridae, genus phlebovirus)
… I established a T7 RNA polymerase-based reverse genetics system to rescue infectious clones of RVFV MP-12 strain entirely from cDNA, the first for any phleboviruses. Using this system, RVFV carrying substitution mutations of the M gene preglycoprotein region (pre-Gn region), one lacking …
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Functional Analysis of Genomic Variation and Impact on Molecular and Higher Order Phenotypes
<p>Reverse genetics methods, particularly the production of gene knockouts and knockins, have revolutionized the understanding of gene function. High throughput sequencing now makes it practical to exploit reverse genetics to simultaneously study functions of thousands of normal sequence variants …
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Investigation into Tissue-Specific Mechanisms of Mitochondrial Dysfunction: Models of SUCLA2 Deficiency and a Screen for Potential Genetic Modifiers
… dysfunction using two genetic approaches. First, reverse genetics tools are used to generate tissue-specific mouse models of succinyl-CoA synthetase deficiency, which is a known cause of mitochondrial disease in humans. In parallel, forward genetics is used to screen for variation in mitochondrial …
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Investigation of the molecular basis of PAMP-induced resistance
… two different approaches were employed. I used reverse genetics to study the involvement of three different secondary metabolites, namely camalexin, glucosinolates and callose, in PAMP-triggered immunity (PTI) against the phytopathogenic bacterium Pseudomonas syringae pv. tomato (Pto) DC3000. …
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Rubisco Activase -- a Typical AAA+ Protein With Unique Features, And, PIFI -- a Novel Chloroplast Protein Functioning in Chlororespiration
… and possibly cyclic electron transport by reverse genetics. In a pifi mutant, which possesses an intact NDH complex, NDH-mediated dark reduction of plastoquinone was disrupted as indicated by its post-illumination fluorescence kinetics. The pifi mutant exhibited low capacity for thermal …
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Identification of Viral Determinants of Murine Norovirus Pathogenesis
… model, can be grown in cell culture, and has a reverse genetics system. Noroviruses are divided into genogroups which can contain many viral strains. These viral strains can display dramatic variation in their virulence and ability to persist, but the factors responsible for these differences …
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A genomic analysis using RNA-Seq to investigate the adaptation of the psychrophilic diatom Fragilariopsis cylindrus to the polar environment
… high-throughput transcriptome sequencing and reverse genetics were applied. Comparative genomics with the sequenced mesophilic diatoms Phaeodactylum tricornutum and Thalassiosira pseudonana was combined with genome-wide RNA-Seq transcriptome analysis, leading to the discovery a new …
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IDENTIFYING HIGHLY CONSERVED PATHOGENICITY GENES IN CHESTNUT BLIGHT AND POWDERY MILDEW FUNGI AS TARGETS FOR NOVEL FORMS OF HOST RESISTANCE
… Cp can help overcome the obstacles to performing reverse genetics on intractable, biotrophic fungi such as Gc. Experiments underway involving the silencing and ectopic overexpression of the Gc homologues of the target genes provide a rapid method to study Cp genes, including to screen additional …
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Design of a microscopy-based screening method for stomatal development genes in <i>arabidopsis thaliana</i>
… The purpose of this project was to create a reverse genetics, microscopy-based screening method to identify Arabidopsis genes important in guard cell development. It was hypothesized that gene coexpression analysis would facilitate identification of gene candidates involved in stomatal …
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Developmental transitions of germ cell lineage of the mouse
… I will present projects utilizing forward and reverse genetics that generate new points of entry into poorly understood transitions during germ cell development. The X and Y chromosome do not have pairing partners during male meiosis. As a result they become silenced during this time. One …
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Plasmodium falciparum protein kinase CK2
… Plasmodium falciparum, seeking to establish by reverse genetics and biochemical approaches whether it represents a possible antimalarial drug target. Protein-kinase CK2, formerly known as Casein Kinase II, is a dual-specificity (Serine/Threonine and Tyrosine) protein kinase ubiquitously …
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Využitie transgénnych rastlín pri štúdiu funkcie rastlinných génov
… the associated function of individual genes. Reverse genetics approaches are used to characterize these genes. In the first place, these include approaches that include the analysis of insertional mutants or the use of RNA interference that inactivates or reduces the expression of the …
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