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Showing 1 to 20 of 75 for “"restriction fragment length polymorphism"”.

  1. Restriction fragment length polymorphism mapping of quantitative and qualitative traits in soybean

    Diers, Brian1991

    <p>The purpose of this thesis was to map traits of interest to soybean (Glycine max (L.) Merr.) breeders and geneticists with RFLP markers. The thesis consists of three separate studies;In the first study, quantitative trait loci (QTL) for seed size, protein and oil content and for the content of …

    iastate Repository record

  2. Assessment of Chemiluminescent Detection System for Forensic DNA Restriction Fragment Length Polymorphism Analysis

    Bush, Paul J.Drake University1984

    … of 32P isotopic detection for human DNA restriction fragment length polymorphism (RFLP) analysis and have the speed, safety, and reduced cost of colorimetric detection. The assessment was made on the basis of: cost, sensitivity, time of operation, ease of operation, and ease of multiple …

    drake Repository record

  3. Restriction fragment length polymorphism analysis of chloroplast DNA, mitochondrial DNA, and ribosomal DNA in turfgrasses

    Zhang, JianhuaVirginia Tech1994

    Restriction fragment length polymorphisms (RFLPs) of chloroplast DNA (cpDNA), mitochondrial DNA (mtDNA), and ribosomal DNA (rDNA), were used to estimate and compare the extent of diversity among the organellar and nuclear genomes, and to infer species relationships. Eight cultivated turfgrass …

    vt Repository record

  4. Estimation of Microbial Diversity in Poultry Litter Using Terminal Restriction Fragment Length Polymorphism and Isolation of Phosphate Accumulating Bacteria from Poultry Litter

    Vadari, Yoganand

    … about the microflora of poultry litter. Terminal restriction fragment length polymorphism of 16S rDNA from bacteria was used to investigate the bacterial diversity of poultry litter. Poultry litter was collected from a local producer. DNA was isolated using commercial kits and amplified using the …

    wku-diss Repository record

  5. BoLA Class II Genes: Polymorphism, Disease Association and Linkage Relationships With the Prolactin and Bola-A Genes

    Van Eijk, Michael Josephus TheresiaUniversity of Illinois at Urbana-Champaign2014

    … II genes of the BoLA complex than with BoLA-A. Polymorphisms of BoLA-DQB and -DRB2 genes were identified using restriction fragment length polymorphism (RFLP) analysis. All carriers of DRB2*2A were found to be resistant to PL (P $<$ 0.005), whereas DRB2*1C homozygotes were at higher risk for the …

    uiuc Repository record

  6. Molecular analysis of algal communities in the San Joaquin River

    Meusburger, Carol Lynn2018

    … similar organisms.</p> <p>The terminal restriction fragment length polymorphism (TRFLP) fingerprinting method, which has been used in previous studies to examine prokaryotic community structure, was modified with the successful algae primers to selectively fingerprint all algal groups in …

    u-pacific Repository record

  7. Effect of invasive phragmites australis and its control on microbial community composition in a freshwater wetland

    Kirk, Jennifer K2018

    … was tested in freshwater wetlands using terminal restriction fragment length polymorphism analysis of PCR amplified eubacterial DNA from soils dominated by Typha and Phragmites vegetation preceding and following herbicide application. Soil bacterial communities differed by vegetation type and …

    emich Repository record

  8. Sickle cell anaemia in Cameroon : co-inheritance of α-thalassemia, HBB gene haplotypes, clinical & haematological characterisations

    Rumaney, MaryamDivision of Human Genetics2015

    … was collected for 262 Cameroonian individuals. Restriction fragment length polymorphism - polymerase chain reaction (RFLP-PCR) was performed for the molecular diagnosis of SCA and for the study of the β-globin (HBB) gene cluster haplotypes. Multiplex Gap-PCR was performed to investigate the …

    cape-town Repository record

  9. Identification and Characterization of a Tolerant Response in Arabidopsis Thaliana to Tobacco Ringspot Virus and Cloning of a Single Locus That Confers Tolerance

    Lee, Jian-MingUniversity of Illinois at Urbana-Champaign2015

    … In the initial mapping, PCR-based short sequence length polymorphism and cleaved amplified polymorphic sequence markers located across the A. thaliana genome were scored on 31 F$\sb3$ families to determine the chromosome location of TTR1. This study placed TTR1 between markers DFR and nga129 on …

    uiuc Repository record

  10. Performance and microbial community structure of Midwestern groundwater pretreatment reactors

    Moor, JohnathanUniversity of Illinois at Urbana-Champaign2013

    … amendment or microbial inoculation. Terminal restriction fragment length polymorphism revealed temporal and depth-related changes in microbial community structure throughout the ripening period in parallel with performance data. This study demonstrates that pretreatment systems could be a …

    uiuc Repository record

  11. Bacterial and archaeal community structure across a gradient of saline lakes in Kiritimati, Republic of Kiribati

    Schmitt, Susan JaneUniversity of Illinois at Urbana-Champaign2016

    … in fresher lakes. Preliminary terminal restriction fragment length polymorphism analysis indicated that samples from the least saline lakes were statistically distinct from the most saline lake microbial communities. Results from Illumina sequencing of 16S rRNA gene amplicons support …

    uiuc Repository record

  12. Introducing the starlet sea anemone Nematostella vectensis as a model for investigating microbial mediation of health and disease in hexacorals

    Har, Jia YiMassachusetts Institute of Technology2009

    … using 16S rRNA gene clone libraries, terminal restriction fragment length polymorphism analysis (T- RFLP), fluorescent in situ hybridization (FISH) and cultivation-based analysis. N. vectensis microbiomes revealed the presence of a novel lineage of epsilon-Proteobacteria associated with animals …

    mit Repository record

  13. Associations of egg production with the major histocompatibility complex in broiler breeder hens

    Tarleton, Becky Jean2019

    … hens. Genomic DNA was isolated from 35 hens. Restriction fragment length polymorphism (RFLP) analysis was performed on each sample, using a chicken class II MHC probe (Xu et al. 1989). Restriction endonuclease digestion using PvuII resulted in polymorphisms in all birds. Five polymorphic bands …

    wvu Repository record

  14. Characterization of the intestinal microbiome and lactobacilli community: influence of dietary and environmental factors

    Chen, LongUniversity of Illinois at Urbana-Champaign2013

    … In Chapter 3, a new protocol of Terminal restriction fragment length polymorphism (T-RFLP) analysis was developed to facilitate lactobacilli composition study. In Chapter 4, both culture dependent methods and the novel T-RFLP tools were used to reveal the impact of piglet age and route of …

    uiuc Repository record

  15. Determining fecal bacterial profiles of a human-habituated wild chimpanzee population in Mahale Mountains National Park, Tanzania

    Szekely, BrianVirginia Tech2009

    … adults. We analyzed fecal samples using Terminal-Restriction Fragment Length Polymorphism (T-RFLP) of amplified 16S rRNA genes to determine bacterial diversity present. Between 1 and 14 terminal-restriction fragments (T-RFs) were observed in each sample. A total of 26 unique T-RFs were produced …

    vt Repository record

  16. Polymerase chain reaction-based genotyping of swine MHC DQB and DRB alleles

    Shia, Yun-ChaoUniversity of Illinois at Urbana-Champaign2011

    … using the PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) analysis technique is described. SLA DQB and DRB locus-specific oligonucleotide primers were designed to analyze expressed genes using the reverse transcription-polymerase chain reaction (RT-PCR) method. …

    uiuc Repository record

  17. Using Molecular Diagnostics Based On Internal Transcribed Spacer 2 Sequence To Study Geographical Distribution of Holarctic Malaria Mosquitoes

    Hodge, James MichaelVirginia Tech2020

    … on GenBank information obtained. An analysis by Restriction Fragment Length Polymorphism using ITS2 PCR products on An. punctipennis was conducted. Seven hundred ninety samples were processed to look at the identity and geographic distribution of Holarctic Anophlines. An. puntipennis has no …

    vt Repository record

  18. New insights on the ecology of free-living heterotrophic nanoflagellates based on the use of molecular biological approaches

    Lim, Ee LinMassachusetts Institute of Technology and Woods Hole Oceanographic Institution1997

    … of nanoflagellate taxa in water samples. Restriction fragment length polymorphism (RFLP) analysis of small subunit ribosomal DNA (SSU rONA) amplified by the polymerase chain reaction (PCR) differentiated pure cultures of heterotrophic nanoflagellates according to established taxonomic …

    woods-hole Repository record

  19. DEPTH AND TIME RELATED VARIATIONS OF MICROBIAL COMMUNIITES IN AN EMERGENT FRESHWATER WETLAND

    Jenkins, Amy2020

    … the microbial community structure via Terminal Restriction Fragment Length Polymorphism (T-RFLP) of 16S-rRNA. Analysis of variance (ANOVA) found significant differences were found between the environmental parameters in regards to site, depth, and season. Three physical-chemical variables (WC, …

    vcu Repository record

  20. Stream microbial communities along an agricultural gradient

    McIntyre Schucker, Cristine

    … extracted sediment and pore-water DNA: terminal restriction fragment length polymorphism (T-RFLP) on the small ribosomal subunit 16S rRNA assessed microbial diversity; and quantitative PCR (qPCR) on amoA, a subunit of the nitrifying gene ammonia monooxygenase, measured nitrifier abundance. A …

    eastern-wash Repository record

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