Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 49 for “"restriction enzymes"”.
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Development and applications of programmable DNA-guided Argonaute-based artificial restriction enzymes
Restriction enzymes or formally known as restriction endonucleases are a class of nuclease enzymes which recognize short DNA sequences and cleave DNA molecules at or near their recognition site. Type II restriction enzymes are capable of cleavage of DNA at a fixed location with respect to their …
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A study of protein-DNA interactions using atomic force microscopy and DNA origami
… such as viruses invading a bacterium. Restriction enzymes defend the host bacteria by recognising specific sites on the viral DNA and cutting invading viruses at those sites. We aim first to understand how these proteins translocate along the viral DNA molecules toward their recognition …
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Chromosomal Fragmentation in Escherichia Coli: Its Absence in mutT Mutants and Its Mechanisms in seqA Mutants
… modification of the phage DNA to protect against restriction enzymes, sensitivity of lysogens to UV damage, and the toxicity when the gam gene was overexpressed in the cell.
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A Novel Method to Detect Bacterial Restriction-Modification (R-M) Systems
… DNA sequences that code for previously unknown restriction endonucleases and their corresponding methyltransferases. These findings show that numerous restriction enzymes abundant in bacteria have yet to be discovered. Traditionally, restriction enzymes have been discovered by the classical …
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The application of artificial intelligence techniques to a sequencing problem in the biological domain
… the order of the genes and other markers called restriction enzymes. It is a time consuming activity, carried out manually by the geneticist. The data from which maps are produced contain a high degree of error, due to experimental limitations, and several feasible solutions may be constructed …
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Learning hierarchical motif embeddings for protein engineering
… model on the DNA recognition code of the Type II restriction enzymes. Based on the promising prediction results, we used the trained models to generate de novo restriction enzymes and paved the way towards the computational design of a restriction enzyme that will cut a given arbitrary DNA …
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Morphological and Biochemical Analysis of Variation in Diploid Glycine Tomentella Hayata (2N = 38, 40)
… from fresh, young leaves and digested by three restriction enzymes. After separation on agarose gels, the Southern blots were probed with three P32 labeled, genomic DNA probes (Chalcone synthase, soybean seed urease, and soybean lectin). Genomic DNA was also amplified with the Polymerase Chain …
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Genetic Analysis of Clostridium Acetobutylicum Using Two-Dimensional PFGE to Construct a Macrorestriction Map of ATCC 824 and Restriction Enzyme Profiles for Strain Analysis
… strains were analyzed using rare-cutting restriction enzymes by one-dimensional and two-dimensional PFGE. C. acetobutylicum strains were placed into three groups based on their restriction enzyme profiles following digestion with SalI, SmaI, and MluI. C. acetobutylicum strains ATCC 824, …
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Characterization of a 4.0 kilobase plasmid from Pasteurella multocida
… was characterized in terms of its size and restriction sites. The restriction patterns produced by fourteen endonucleases were used to generate a restriction map. Five restriction enzymes cleaved the plasmid at multiple sites. Two enzymes, Bgl II and Sal I had unique sites on pLAR-1. Twelve …
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Covalent DNA Modifications in Phage and Bacterial Dynamics
… nucleases that restrict infection, such as restriction enzymes and CRISPR systems, and the covalent DNA modifications that neutralize them. Multiple targeted nucleases equip bacteria with an innate immune response against phage, and CRISPR systems provide an adaptive immune response. I …
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Species-specific DNA probes for the identification of Actinobacillus actinomycetemcomitans
… from A. actinomycetemcomitans, digested with a restriction enzyme, Sau3A, ligated to plasmid DNA (pUC18) and transformed into JM109 cells to give a partial A. actinomycetemcomitans library. The library was screened using Southern blot analysis. Out of the nine inserts tested, one was found to be …
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Role of nuclear factor kappa B (NFkB) in the vascular complications of diabetes.
… line using polymerase chain reaction (PCR) and restriction enzymes. Methods: Genetic polymorphisms were investigated using bioinformatics tools through computational analysis and databases, such as, Ensembl and the National Center for Biotechnology Information (NCBI), NEB cutter, Primer Quest …
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Restriction Enzyme Analysis of Incomplete Parental Replicative Forms of øX174
… contradict that interpretation.</p> <p>Using restriction enzymes we have re-examined the incomplete RF made from UV-damaged phage. Parental RF made <em>in vivo</em> from viruses exposed to various UV doses were purified by velocity sedimentation and benzoylated naphthoylated …
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Bacterial defense islands: CRISPR-Cas and toxin-antitoxin systems /
… molecular tools for genetic engineering, such as restriction enzymes, bacterial argonautes and CRISPR-Cas systems. CRISPR-Cas systems are located in the genome part called “defense islands” where genes encoding different antiviral systems are clustered together. The goal of this thesis is to …
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On the Repair of DNA Breaks and the Specificity of the EcoRI Restriction Enzyme
<p>We would like to understand how proteins and enzymes interact with DNA. We describe here our studies of the Mrr methylation dependent restriction system, DNA single- and double-strand break repair in E. coli, and substrate recognition by the EcoRI endonuclease. Many species of bacteria make …
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Initiation of Translation of Eukaryotic Messenger-Rna at a Unique Site Lacking an AUG Codon
… Linearization of these plasmids with appropriate restriction enzymes followed by runoff transcription yields short mRNAs which code for defined proteins. This approach allows study of the role of specific nucleotides or nucleotide combinations in the initiation of translation. Surprisingly, such …
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On the epigenetic ageing clock in humans
… more cost-effective by exploiting the ability of restriction enzymes to perform genomic enrichment. This thesis provides novel insights (statistical, biological, technological) into the epigenetic ageing clock in humans, which will help to shed light on the different processes that erode the human …
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Evaluation of hybridization among three Laricobius species, predators of hemlock woolly adelgid, (Adelgidae)
… L. osakensis from L. naganoensis. Three restriction enzymes were found that correctly differentiated the two species.
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Microbiological evaluation of the Mhlathuze River in KwaZulu-Natal
… C. freundii and C. ctversus. Rsa I and Hinf I restriction enzymes were also used for double digestion ofE.coli and P. vuigaris.
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Studies on the mitochondrial DNA of Tetrahymena
… str. W/ATCC. was isolated and mapped using six restriction enzymes. The linear molecule was seen to be approximately 35 Md. in size, slightly larger than the mtDNA seen in other Yetrahymena strains. The T. furgasoni mtDNA molecule also showed a heterogeneity in the length of its terminal …
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