Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"response regulators"”.
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Role of the Nla6S and Nla28S histidine kinases in fruiting body development of <i>Myxococcus xanthus</i>
… developmental processes in bacteria. In response to adverse environmental conditions, M. xanthus aggregates and forms multicellular structures known as fruiting bodies. Two component signal transduction systems (TCS) are widely used by bacteria to detect and respond to environmental cues …
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Studies on Structure and Dynamics of Components of Phytochrome Mediated Light Signaling in Plants and Cyanobacteria
… the crystal structures of two cyanobacterial response regulators (RR), which directly interact with bacterial phytochrome receptors, have been solved. This could be accomplished by heterologous expression, purification and crystallization of the proteins, and X-ray structure determination, …
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Mechanism and specificity of bacterial two-component signaling systems
… often hundreds of the histidine kinase (HK) and response regulator (RR) pairs that make up a TCS system can be found in a single cell. How do these systems maintain faithful signal transmission and avoid cross-talk? To understand how specificity is determined, we examined co-evolving residues …
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Regulation of clostridium acetobutylicum glutamine synthetase glnA gene
… the amino terminal region of the DegU and CheB response regulators, respectively. These regulators belong to the family of the response regulators involving two component signal transduction systems, suggesting that the protein encoded by this ORF may play a role in the mechanism of regulation …
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Structural Characterization of Two-Component Signal Transduction Proteins and Calbindin D28k
… properties of the initiation of sporulation response regulator Spo0F was studied using a variety of biophysical and biochemical techniques. The experiments show that most of the divalent metals studied, including and Ca²⁺, Mg²⁺ and Mn²⁺, which display primarily 1:1 binding, allow for …
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Binding with intent to destroy : RssB adaptor function in Clp-XP-mediated proteolysis of sigmaS
… subunit of the RNA polymerase is a master stress response regulator in E. coli. Under satisfactory growth conditions, proteolysis keeps the levels of as low; upon stress, various mechanisms converge to raise the levels as. In this work we focus on the facilitated delivery of as to the ClpXP …
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Applying systems-thinking to reduce check-the-box decisions in the audit of complex estimates
Even as audit regulators push for increased use of professional judgment in the audit of complex financial statement accounts, auditors seem to interpret audit standards as increasingly prescriptive. This leads to mechanical, or check-the-box audit decisions in just those situations in which it is …
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Evolution-Inspired Design of Bacterial Biosensors
… sensor histidine kinases (SKs) and their cognate response regulators (RRs) using the protein language model ESM2. We show that the model is able to identify highly coevolving residues at the SK-RR interface. We then train a dedicated pairing model to predict TCS interactions from their amino acid …
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Biophysical and Biochemical Characterization of a REC Domain: Unfolded to Folded Transition of EL_LovR
… to couple environmental stimuli to adaptive responses. These pathways use histidine kinases to detect environmental cues, harnessing these to control phosphorylation of the receiver domain of the response regulator, which convert this signal into a physiological response. Knowledge of how …
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Novel Diagnostic Methods for Rapid Detection and Identification of Bacterial Infections in Bovine Mastitis
… conserved species-specific primers designed from response regulators which were combined with a portable PCR system and a diagnostic dipstick for the rapid detection of species specific DNA. For the third method a whole-cell bacterial biosensor was constructed for different mastitis pathogens, …
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The role of post-transcriptional regulators in pathogenesis and secondary metabolite production in Serratia sp. ATCC 39006.
… regulation that plays a key role in stress response and virulence in other bacterial species. An S39006 ∆hfq mutant was constructed and in the mutants production of prodigiosin and carbapenem was abolished, while production of the QS molecule, butanoyl homoserine lactone (BHL), was …
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Serotype Association and Regulation of the Hyaluronate Lyase Gene of <i>Streptococcus pyogenes</i>
… a primary defense against the host immune response. The <em>hyl</em>A gene, which encodes the enzyme, has been shown to take three structural forms: a full-length gene, one containing a 3' deletion and a gene that produces a prematurely truncated protein. This work was intended to show that …
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Identifying Novel Factors Involved in Biofilm Formation by Vibrio Fischeri
… locus, as well as the sensor kinase RscS and the response regulators SypG, required for syp transcription, and SypE, a negative regulator of biofilm formation. However, I predicted that other factors were also involved in biofilm formation. Therefore, my dissertation work focused on identifying …
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Regulation of desiccation tolerance in Xerophyta seedlings and leaves
… and 5%) to identify the networks activated in response to water loss. Desiccation was associated with successive waves of transcription factor induction, as well as widespread down-regulation of histone modification enzymes. Many seed-specific genes, such as late embryogenesis abundant (LEA) …
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Characterization of Two Genes Up-regulated During Heterocyst Development in the Cyanobacterium Anabaena sp. strain PCC 7120.
… while that of all4312 suggests it encodes the response regulator of a two-component regulatory system. Phylogenetic analysis of the predicted protein sequences of alr4311 and all4312 indicated that both of these proteins have orthologs in Nostoc punctiforme and Anabaena variabilis, two …
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Developmental Characterization of Cara Mitad: A Drosophila Nuclear Receptor Co-Regulator
… activities, we lack essential knowledge of the coregulators that are required for their proper function.</p><p>We have recently identified the Drosophila cara mitad (cmi) (`dear half') gene. The deduced CMI protein is closely related to the N-terminal half of the ALR-1/MLL-2 vertebrate NR …
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The Functional Characterization of QSEC a Bacterial Adrenergic Receptor and the LuxR Homologue SDIA in EHEC
… the flagellar genes through its cognate response regulator QseB. Here, we examined the global role of QseC in EHEC gene regulation. Microarray analysis of ΔqseC along with real time RT-PCR (qPCR) revealed QseC’s regulation of Stx, NleA, and Ler, the master regulator of the LEE. …
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Studies on the role of CheS in Sinorhizobium meliloti chemotaxis
… kinase CheA, which is autophosphorylated in response to a signal from a ligand-bound transmembrane methyl-accepting chemotaxis protein. CheA transfers the phosphate group to its cognate response regulator which modulates flagellar rotation. Signal termination by dephosphorylation of the …
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Untersuchungen zur Rolle des Phosphoproteins Stathmin in Listeria monocytogenes-infizierten Säugerzellen und Molekulare Charakterisierung der listeriellen Zweikomponetensysteme
… Mutanten konstruiert, in denen individuelle Response Regulatorgene deletiert sind. Die erhaltenen Mutanten wurden in vitro und in vivo auf ihr Wachstumsverhalten hin untersucht. Es zeigte sich, dass unter den angewandten Kultur- und Versuchsbedingungen keines der Zweikomponentensysteme eine …
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Similarities and variations of the enterobacterial chemotaxis paradigm in Sinorhizobium meliloti
… protein, CheT, on phosphorylated S. meliloti response regulators. The kinase CheA phosphorylates both the sink response regulator, CheY1, and the flagellar motor interacting response regulator, CheY2. CheY1 competes with CheY2 for these phosphate groups, but we have discovered another layer of …
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