Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 267 for “"resonance energy transfer"”.
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Fluorescence Resonance Energy Transfer (FRET) Based Sensors for Bioanalysis
… new methods and sensors based on fluorescence resonance energy transfer (FRET) for bioanalysis. Chapter 3 describes the use of FRET between donor fluorophores and acceptor labeled murine macrophage cells. FRET microscopy was used to determine whether the donor molecules truly permeate through …
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Fluorescence Resonance Energy Transfer (FRET) systems for biomedical sensor applications
This thesis investigates the use of Fluorescence Resonance Energy Transfer (FRET) for biomedical sensor applications. FRET is a process by which energy is transferred, via long range dipole-dipole interactions, from a donor molecule (D) in an excited electronic state to an acceptor molecule (A). …
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Conformational Studies of Myosin and Actin with Calibrated Resonance Energy Transfer
Resonance energy transfer was employed to study the conformational changes of actomyosin during ATP hydrolysis. To calibrate the technique, the parameters for resonance energy transfer were defined. With conformational searching algorithms to predict probe orientation, the distances measured by …
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Forster Resonance Energy Transfer in materials used in Luminescent Solar Concentrators
… of the molecule absorbs the sun’s radiation and transfers it to the acceptor. The acceptor molecule is the emitting fluorophore which is shifted to lower energy reducing the chances of reabsorption. Covalently linked donating and accepting fluorophores will act as an energy down-converting system …
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Förster resonance energy transfer (FRET)-based nanophotonics using DNA origami structures
… The elements of nanoscale photonic devices can transfer excitation energy non-radiatively from an excited donor molecule to an acceptor molecule by Förster resonance energy transfer (FRET). The efficiency of this energy transfer is highly dependent on the donor-acceptor distance. Hence, in these …
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Fluorescence resonance energy transfer-based biosensors for monitoring prostate specific antigen
… regions of prostate tissue. Using fluorescence resonance energy transfer (FRET), several peptide substrates have been designed to respond to varying concentrations of PSA with a concomitant increase in fluorescence. In the near-infrared wavelength range, these fluorescent substrates can be …
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USING CONJUGATED POLYMERS AS BIOLOGICAL SENSOR BASED ON FLUORESCENCE RESONANCE ENERGY TRANSFER
… novel molecular sensors based on fluorescence resonance energy transfer (FRET) between fluorophore (donor) and polydiacetylene (PDA, acceptor) for selective detection of biomolecules in solution. The work described in this dissertation is divided into three sections. In the first section, we …
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Single-molecule fluorescence resonance energy transfer study of SNARE-mediated membrane fusion
… fusion through single-molecule fluorescence resonance energy transfer (smFRET). Membrane fusion is one of the important cellular processes by which two initially distinct lipid bilayers merge their hydrophobic cores, resulting in one interconnected structure. For example, exocytosis, …
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Conformational Dynamics of the Glutamate Receptors via single Molecule Förster Resonance Energy Transfer
… it describes the use of single molecule Förster resonance energy transfer to observe the bound form of the glutamate receptors and subsequent dynamics. For a complete understanding of the dynamics involved in the conformational landscape, the response of the receptor to a variety of known full …
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An Investigation of the Phospholamban-Serca Regulatory Interaction with Fluorescence Resonance Energy Transfer
<p>With Fluorescence Resonance Energy Transfer (FRET), we are able to detect changes in the structure and affinity of the PLB-SERCA regulatory complex in live cells. Using this approach, we have detected a high level of PLB-SERCA interaction even at Ca2+ concentrations known to fully relieve PLB …
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Optimization and Applications of Fluorescence anisotropy assays and Fluorescence Resonance Energy Transfer Measurements
Calmodulin (CaM) is a calcium signaling protein that activates over hundred of targets including PMCA. This dissertation mainly focuses on optimizing and applications of fluorescence anisotropy (FA) and FRET experiments for CaM-target interactions. First we evaluated the extent of interaction of …
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Luminescence Resonance Energy Transfer Studies of the Shaker Potassium Voltage-Gated Ion Channel
… sensing. We use a technique called Lanthanide Resonance Energy Transfer (LRET), which gives an accurate distance measurement between two positions on the ion channel. We use two different configurations for LRET on Shaker. The first measures distances between the four identical S4 amino-acid …
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Luminescence resonance energy transfer studies of the Shaker K+ voltage-gated ion channel
Submitted by Meng Tao (mengtao2@illinois.edu) on 2012-10-26T23:06:21Z No. of bitstreams: 1 Posson_David.pdf: 7844031 bytes, checksum: 3cee2902577cf067066066ebcaad3557 (MD5)
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Investigations of The Structure-Function Relationship In Kainate Receptors Using FöRster Resonance Energy Transfer
… work in this dissertation utilizes luminescence resonance energy transfer (LRET) and single-molecule Förster Resonance Energy Transfer (smFRET) in order to address this gap in the knowledge. We have characterized the structural arrangement and dynamics of the homomeric (GluK2) receptors and …
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Ratiometric copper(II) indicators based on poly(n-isoproprylacrylamide) and fluorescence resonance energy transfer
… brings about a large increase in fluorescence resonance energy transfer (FRET). Addition of Cu(II) to the aggregated copolymer introduces charge onto the backbone, causing the copolymer to deaggregate with a resulting decrease in FRET. The ratio of acceptor to donor fluorescence varies with …
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Study Of Protein-Rna Interactions Using Fluorescence Resonance Energy Transfer (fret) And Single-Molecule Fret
… poorly understood. Single-molecule Fluorescence Resonance Energy Transfer (smFRET) techniques that provide information about heterogeneity and dynamic behaviors of molecules have been developed to investigate inter- and intra-molecular interactions. Here we have used FRET in combination with …
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In Vivo Investigation of Protein Interactions in Caenorhabditis Elegans by Forster Resonance Energy Transfer Microscopy
In the second part of the thesis interactions of PAT-4, the C. elegans homologue of integrin-linked kinase (ILK), were investigated by FRET microscopy in living transgenic animals expressing CFP- and YFP-fusion proteins. Quantitative FRET microscopy in living C. elegans was most complicated by …
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