Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"residue-specific"”.
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Translational Incorporation of Unsaturated Amino Acids
… This thesis is concerned with the in vitro residue-specific incorporation of unnatural amino acids to produce proteins and peptides with increased functional diversity and potential applications in biotechnology. The cellular lysate from E.coli is a common source of translational machinery …
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Translational Incorporation of Unsaturated Amino Acids
… This thesis is concerned with the in vitro residue-specific incorporation of unnatural amino acids to produce proteins and peptides with increased functional diversity and potential applications in biotechnology. The cellular lysate from E.coli is a common source of translational machinery …
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Ion Mobility and Gas-Phase Covalent Labeling Study of the Structure and Reactivity of Gaseous Ubiquitin Ions Electrosprayed from Aqueous and Denaturing Solutions
… the intact, labeled protein to determine which residues were labeled. Ubiquitin 5+ and 6+ electrosprayed from aqueous solutions were covalently modified preferentially at the lysine 29 and arginine 54 residues, indicating that elements of secondary structure, as well as tertiary structure, were …
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NMR-Based Characterization of Proteins: Structure, Dynamics and Binding Interfaces of Influenza A's M1
… of NMR-accessible timescales and facilitates residue-level mapping of molecular surfaces involved in intermolecular recognition. The first two chapters of this dissertation focus on the theoretical background required for interpreting NMR experiments. Chapter 1 introduces the fundamentals of …
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Development of new parameters for structure determination and dynamic investigations on biomacromolecules by NMR
… means of NMR-spectroscopic techniques, targeting specific problems as well as contributing to the general understanding and providing new, widely applicable methods. The main focus was on the structural as well as dynamic study of ribonucleic acids (RNA). A new structural NMR method was developed …
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Structural and Biophysical Characterisation of Denatured States and Reversible Unfolding of Sensory Rhodopsin II
… binding pocket is disrupted, with transmembrane residues becoming more solvent-exposed. Folding of pSRII from an SDS-denatured state harbouring a covalently-bound retinal chromophore shows deviations from an apparent two-state behaviour. SDS denaturation to form the sensory opsin apo-protein is …
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Regulation of the FGF14:Nav Interactome by a GSK-3 Centered Pathway
… protein signaling and genetic programs through residue-specific phosphorylation. Recent studies have shown that the assembly and trafficking of FGF14, an intracellular fibroblast growth factor critical for the maintenance of the Nav complex, in neurons is controlled via a GSK-3 dependent …
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Effects of macromolecular crowding and small ions on the folding, structure, and stability of Desulfovibrio desufuricans flavodoxin
… structural and mechanistic information with residue-specific resolution. We also found that small cations and anions in physiologically relevant concentrations (≤ 250 mM) increase flavodoxin thermal stability significantly. Both cations and anions in higher concentrations (300 mM-.75 M) …
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Investigation of asparagine-linked glycosylation in archaeal and bacterial systems
… inhibitors. In addition, treatment of PglB with residue-specific alkylating agents coupled with site-directed mutagenesis revealed a key histidine residue that may play an important role in enzyme catalysis. Taken together, these studies offer insight into long-standing questions about the …