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Showing 1 to 20 of 23 for “"resazurin"”.
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Physical, chemical, and microbial dynamics in the hyporheic zone
… In chapter 4, I explore the reactive tracer resazurin and its use for measuring respiration. I explore the molar ratio between resazurin conversion and respiration, and how this can be used to quantify hyporheic respiration rates in real-world systems. Finally, in chapter 5, I show a few …
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Phytochemical and Antimicrobial Studies on Selected Medicinal Plants from The Iraqi Flora: Citrus grandis, Citrus sinensis and Ruta chalepensis
… antimicrobial proprieties using the microtitre resazurin assay involving two Gram-positive bacterial strains, Micrococcus luteus and Staphylococcus aureus, two Gram-negative strains, Escherichia coli and Pseudomonas aeruginosa and one fungal strain, Candida albicans. The DCM extracts of C. …
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Evaluation of potential photodynamic therapy agents and patient-relevant biomarker combinations for the selective targeting of cancer
… controls. Finally, we investigated the resazurin assay, a method used for the evaluation of proliferation and cytotoxicity in more than 2,000 publications. We found that only ~14% of these utilized validated assay conditions, while ~40% failed to report essential analytical parameters …
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Investigation of the efficacy of identified acetolactate synthase inhibitors, peptidyl cysteine protease inhibitors, thiolactomycins, and thiosemicarbazone compounds against Mycobacterium tuberculosis
… vitro efficacy against M. tuberculosis using the resazurin microtitre plate assay afterwhich active compounds were assessed for cytotoxicity in vitro against elicited peritoneal macrophages using the MTT assay. Of the 38 acetolactate synthase inhibitors tested, 2 derivatives namely RKG162A and …
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Development and operation of a perfusion bioreactor for the cultivation of mammalian cells inside a sponge-like ceramic matrix
… Wachstum über die metabolische Reduktion von Resazurin und Masse elementaren Kohlenstoffs, Zellverteilung und -vitalität über Schnittfärbungen nachgewiesen wurden. Überdies wurden in den Poren der Keramiken mittels Rasterlektronenmikroskopie proteinartige Strukturen gefunden, die den Schluss …
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Optimizing alginate-chitosan microcapsules using co-axial air flow method as 3d stem cell microenvironment
… is tested by means of live-dead cell and resazurin assays of encapsulated cells. The proliferation and differentiation of encapsulated mouse OCT4-GFP embryonic stem cells are analyzed by flow cytometry. It is shown that encapsulated cells are able to remain viable and that the microcapsule …
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Comparative Study of Milk Quality Tests on Manufacturing Grade Raw Milk
… count, methylene blue reduction test, and resazurin reduction test were the tests used in this study. The medium of comparison was raw bulk tank milk of manufacturing grade. It was the goal of this research to assess how the present quality tests compare in their ability to determine the …
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HIF-1alpha role in oxygen-dependent radio- and chemosensitivity
… (after 7 days) was measured in terms of resazurin reduction. Oxygen-dependent radio- and chemosensitivity was evident in all wild type whereas it was reduced or abolished in HIF-1α (siRNA) knockdown cells. The effects of HIF-1α-modulating drugs (EDHB, CoCl2, deferoxamine to stabilise and …
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Development of microelectrode arrays for real-time monitoring of alkaline phosphatase release from cells in clinical analysis
… of the target sample was performed by using resazurin assay. The standard method for ALP detection was used for optimizing the conditions of the enzymatic assay during ALP release. Balb/c 3T3, A549 and MCF-7 cells displayed an optimal initial density of 40×103 cell/mL, whereas the Ht20 cells …
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Investigation of anthracycline based topoisomerase II inhibitors in triple negative breast cancer
… diphenyl tetrazolium bromide and resazurin assay. The results of KP71 demonstrated DNA damage through Western blotting, inhibition of topoisomerase IIα and IIβ via the decatenation assay, and suppression of tubule formation in angiogenesis using the organotypic co-culture assay, …
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An evaluation of some commercially-available thin TiO2 films and TiO2 films grown by atomic layer deposition for potential photocatalytic applications
… to decrease in respect to basic blue 66 and resazurin ink photodegradation, as well as increasing water contact angle hydrophobicity under UV light irradiation. However, the permanent damage to photocatalytic activity decrease was not incremental after multiple treatments for the non-residue …
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Soft matter engineering for cellular bioelectronic interfaces
… rare, highly electrogenic cyanobacteria using Resazurin, a redox-sensitive fluorescent-marker. The platform was used for the isolation, recovery and growth of a previously-unknown highly-reducing cyanobacterial strain. Shifting the focus from charge generation to charge provision, a second …
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Anticancer activity of Lovage (Levisticum officinale Koch) extracts
… prepared and tested for cytotoxicity using a resazurin metabolic indicator assay. The most cytotoxic extract was the hexane, followed by the ethyl acetate, while the methanol and the methanol/water extracts demonstrated no cytotoxicity. Further fractionation of the n-hexane and the ethyl …
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Development and testing of thin photocatalyst films
… as well as the reductions of methylene blue and resazurin, in solution and ink forms. The photoactivity of TiO<sub>2</sub>-SEN film is measured against Pilkington Activ™ glass for each of these reactions, which is a commercial product that has a TiO<sub>2</sub> film analogous to the films made in …
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Studies using luciferase-expressing Leishmania donovani parasites
… drug screening was determined using a resazurin based assay incubated with amphotericin B, 1-0.005μg/ml; miltefosine, 20-1.25μg/ml; paromomycin, 300-17.5μg/ml. Furthermore, insertion of either gene into either parasite strain did not result in any change in susceptibility of …
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The use of non-ionic surfactant vesicles to prevent radiocontrast media-associated damage
… and vascular cells using assays including the Resazurin metabolic indicator assay, thymidine-incorporation, CellTox Green™ assay and ApoToxGlo™ assay. IOX-NIVs were reproducibly produced (n = 3) with an average size of 204 ± 24 nm, low PDI of 0.13 ± 0.04, surface charge of -26 ± 1 mV and EE of …
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Microreactor synthesis of labelled polyphenols : a route to antibacterial modes of action in important hospital pathogens
… Bacterial viability was tested using the resazurin indicator method. No viable cells were observed from bacterial samples exposed to greater than or equal to 4 hours of the continuous flow of theaflavin products. This indicated that the theaflavin produced antibacterial action after this …
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The in vitro effects of diallyl trisulfide on osteoclast formation and function in RAW 264.7 murine macrophages
… in RAW 264.7 murine macrophages. Using a resazurin assay, 1-5 µM of DATS was shown not to affect the viability of the RAW 264.7 murine macrophages. RAW 264.7 murine macrophages were then differentiated into osteoclasts using RANKL and stained for tartrate-resistant acid phosphatase (TRAP). …
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Putative phosphodiesterase inhibitors as potential new chemotherapies against African Trypanosomiasis
… TbAT1 knockout, was established by the standard resazurin reduction assay. 25 compounds from Series 1 had EC50 values below 0.5 µM, with 7 under 100 nM and the most active having an EC50 value of 5.8 ± 3.4 nM. For the much smaller Series 2 (GJS Compounds), the most active compound was GJS-128 …
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The effect of the cyanobacterial toxin saxitoxin on neurodevelopment
… in Chapter 3; “Optimisation of a real time resazurin based assay for use in OVCAR-3 and SH-SY5Y cells”. Moving to a model which more accurately models mammalian neuronal differentiation, the effect of STX at the drinking water guideline (3μg/L) and a predicted algal bloom concentration …
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