Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 79 for “"reporter system"”.
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A Dual-GFP Reporter System for Studying Bi-Directional Promotion
… transcription. Green Fluorescent Protein (GFP) reporters may be suitable for simultaneous measurement in a dualreporter system. GFP variants have similar half-lives, sensitivity for detection, and distinct excitation and emission wavelengths. The GFP variant, ECFP and EYFP, were chosen as …
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Predictive Reporter System for Investigating Dose Dependency in Oncogene-Induced Senescence
… To address this question, we developed a cell system where a constitutively active fluorescence marker (mVenus) stoichiometrically reflects the level of inducible oncogenic HRASG12V. This allows pre-induction sorting into subpopulations expressing differing levels of mVenus, which is reflected …
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Development of a high throughput reporter system using β-Galactosidase in the yeast : Pichia Pastoris
… that make for a powerful heterologous expression system. This study focuses on harnessing the benefits described to develop a high-throughput reporter system for the screening of potential super-secreting mutant strains of P. pastoris. Plasmid constructs were engineered with the lacZ reporter …
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The development of a new reporter system to study the interaction between FbpA and FbpBC
Bacteria require efficient iron acquisition systems to infect and colonize the mammalian host. Neisseriaceae and Pasteurellaceae have developed an iron uptake system that involves iron removal from host glycoproteins. The human pathogen, Haemophilus influenzae, has a surface receptor complex …
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Imaging Myelin Basic Protein expression in a model of remyelination by Magnetic Resonance Imaging using an Organic Anion Transporter Protein gene reporter system.
… It demonstrates that, by using a novel MRI gene reporter system; OATP, and controlling its expression under a Myelin Basic Protein promoter, OPCs can take up gadolinium based contrast, and be detected using T1 weighted MRI imaging in vitro and ex vivo. Using a constitutively active promoter, …
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Development of a reporter system for the study of gene expression for solvent production in Clostridium beijerinckii NRRL B592 and Clostridium acetobutylicum ATCC 824
… study the regulation of gene expression, a good reporter system is very useful. The lack of a good reporter system for the solvent-producing clostridia hindered the progress of research in this area. The objective of this study was to develop a reporter system to facilitate the elucidation of the …
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DEVELOPMENT OF A RAPID DIAGNOSTIC ASSAY FOR THE COLORIMETRIC DETECTION OF BACTERIAL AND VIRAL PATHOGENS USING LOOP-MEDIATED ISOTHERMAL AMPLIFICATION COUPLED WITH A PEPTIDE NUCLEIC ACID AND GOLD NANOPARTICLE REPORTER SYSTEM
… probe and gold nanoparticle (AuNP) detection system that results in a colorimetric assay. By employing modified LAMP primers, the target-dependent, co-amplification of a novel, sequence is generated, which serves as the target for PNA/AuNP sequence-specific detection. This investigation …
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Quantification of microRNA regulation and its consequences at the single cell level
… This thesis focuses on a microRNA activity reporter system to investigate various aspects of miRNA regulation on its endogenous 3'UTR targets. Mutation of selected MREs on 3'UTRs (MutUTRs) was designed and validated as miRNA unregulated control. It does not require genetic modifications of …
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Before and after DUX4: deconstructing its web of silencers and defining its long-term impact on cellular processes
… Here, I designed a functional silencing reporter system to identify specific D4Z4 sequences that drive silencing activity and discovered that one discrete sequence, termed D4Z4-S5, was sufficient for sequence-dependent silencer recruitment. This sequence drives silencing activity through …
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Investigation of natural genetic modifiers of meiotic crossover frequency in Arabidopsis thaliana
… region of the genome flanked by a fluorescent-reporter system. F1 plants were then self-fertilised to produce segregating mosaic F2 populations for mapping. Recombination frequency for specific genomic intervals was determined for each individual in the population through measurement of the …
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Investigation of Streptomyces promoters
… to develop a computer assisted analysis system whereby potential promoter sequences could be determined and to use this in the analysis of the cloned promoters. Initially the suitability of the Streptomyces antibioticus me1C operon for use as a reporter system in Streptomyces was …
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A Two-colour Reporter Screen and Application to Cell Cycle Transcription
Development of genome-wide reagents has allowed systematic analysis of gene function. The experimental accessibility of budding yeast makes it a test-bed for technology development and application of new functional genomic tools and resources that pave the way for comparable efforts in higher …
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On the Origin of Phenotypic Variation: Novel Technologies to Dissect Molecular Determinants of Phenotype
… part of my work describes the creation of a reporter system for DNA methylation for the purpose of dissecting the genetic contribution of tissue-specific patterns of DNA methylation across the genome. Finally the last part of my work is focused on understanding the basis of stochastic …
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Tsyn-Seq: A T Cell Synapse-Based Antigen Identification Platform
… antigen screening method, the T-synapse (Tsyn) reporter system, which includes antigen-presenting cells (APCs) with a Fas-inducible NF-κB reporter and T cells with a nuclear factor of activated T cells (NFAT) reporter. To functionally screen for target antigens from a cDNA library, productively …
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Investigation of the Function and Regulatory Mechanisms of the Mammalian Target of Rapamycin
… of mTOR cytoplasmic-nuclear shuttling. A systematic screen of mTOR revealed a rev-like NES sequence within mTOR at amino acids 1281--1289, which displayed robust nuclear export activity in a reporter system. Mutation of the conserved leucines within this export site resulted in a loss of …
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CRISPR-based approach enables targeted random nucleotide diversification in mammalian cells
… have been mainly developed for bacterial systems, preventing the evolution of proteins in higher organisms in their cellular context. The only tools available for mammalian cells rely on the AID cytidine deaminases, able to promote only G and C nucleotide diversification. Here we present …
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Expression of Human Estrogen Receptor and Studies of Estrogen-Mediated Transcription and Apoptosis
… cells. Using transient transfections and a reporter system to measure p38 activity, we show that E2 induces the p38 activity 12--36 fold in several ER positive cell lines, while OHT induces p38 activity 2--5 fold. A selective inhibitor of p38 MAP kinase, S13 203580, partially protects …
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Studies on the regulation of nitrogen metabolism in clostridium acetobutylicum NCP 262 and clostridium beijerinckii NCIMB 8052
… to demonstrate a regulatory role for glnR in a system using the cloned C. acetobutylicum NCP 262 ginA with and without glnR in the E. coli glnAntrBC mutant YMCll. Although the enzyme was efficiently expressed and fully functional, no evidence could be found of any influence of glnR on GS …
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A functional role of chromatin remodeling factor, Rsf-1 in the DNA damage signaling pathway
… micro-irradiation and stably integrated reporter system at a single DNA double-strand break). Rsf-1 rapidly accumulated at the DSB sites. Signal of γ H2AX is gradually reduced at 10 minutes after micro-irradiation whereas signals of Rsf-1 and SNF2h are still retained over 30 minutes after …
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