Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 47 for “"reporter genes"”.
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Photoacoustic Tomography of Diabetes and Reporter Genes
<p>Photoacoustic Tomography (PAT) is a hybrid technology which combines light absorption contrast with ultrasonic detection to achieve deep imaging of normally obscured absorbing molecules in tissue. PAT's inherent background free, super-depth imaging capability makes it particularly well suited …
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A Dual-GFP Reporter System for Studying Bi-Directional Promotion
… transcription. Green Fluorescent Protein (GFP) reporters may be suitable for simultaneous measurement in a dualreporter system. GFP variants have similar half-lives, sensitivity for detection, and distinct excitation and emission wavelengths. The GFP variant, ECFP and EYFP, were chosen as …
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Studies on the mechanisms of action of the male specific lethal complex in Drosophila
… MOF, to nullify the overexpression of autosomal genes but allows this effect to act on the X chromosome. The X linked genes in males are up-regulated twofold to reach the level of two X chromosomes in females. To investigate the repressive property of the MSL complex, selected protein components …
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Identification of Regulatory Binding Sites and Corresponding Transcription Factors Involved in the Developmental Control of 5'-nucleotidase Expression in Dictyostelium discoideum
… the expression levels of a large number of genes by utilizing available gene regulation mechanisms. The developmental control of 5â -nucleotidase (5nt) expression in the model system Dictyostelium discoideum has provided a focal point for studies of gene regulation at the level of …
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Screening for fluorescent and chromoproteins from South African sea anemones
… recovery of anti-cancer drugs, antibiotics and reporter genes (Faulkner, 2000; Allen and Jaspars, 2009). The most commercially lucrative products from sea anemones are fluorescent and chromoproteins (FP/CP), which are used as non-invasive real-time reporter genes. The applications for these …
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Novel Approaches to Transgenesis in the Teleost Medaka (Oryzias latipes)
… of transgenic medaka fish. General transgenesis including transient expression of reporter genes and germ line integration of reporter genes was improved by application of two novel techniques. In addition, one of these methods allows for the first time efficient enhancer trapping in …
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PolyA signals located near 5’ of genes are silenced by a general mechanism that prevents premature 3’ end processing
PolyA signals located at the 3’ end of eukaryotic genes drive the cleavage and polyadenylation reaction to the nascent pre-mRNA. Although these sequences are expected only at the 3’ end of genes, we found that strong polyA signals are also present within the 5’ untranslated regions (UTRs) of many …
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Studies of Estrogen Receptor's Roles in Tamoxifen-Induced Apoptosis and the Regulation of Gene Expression
… estrogen response element (ERE)-containing reporter genes, I isolated stably transfected cell lines that express the ERalphaS118A phosphorylation site mutant. Using stably transfected HeLa cell lines with functional ERK1/2 activity and expressing similar levels of wild-type ERalpha and …
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ELUCIDATING REGULATORY NETWORKS PROMOTING B-CELL DEVELOPMENT
… expression of surface markers and transgenic reporter genes, we have identified three hierarchically related and functionally distinct subpopulations within the common lymphoid progenitor (CLP) compartment. Using this knowledge, we have re-evaluated previously characterized knock-out mouse …
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Autoregulation of the Glial Gene reversed polarity in Drosophila melanogaster
… or glia. I seek to understand more about the genes that control this process, focusing on the lesser understood of the cell types, glial cells. Using Drosophila melanogaster as a model system, previous work from my lab and others has established the role of the master regulatory transcription …
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Construction of an efficient secretion system for recombinant proteins in Bacillus subtilis
… in Bacillus subtilis using an a-amylase as a reporter enzyme, which is secreted into the medium using the Sec pathway. Its gene amyQ was fused to an IPTG-inducible promoter. It turned out that increasing amounts of IPTG did not result in a concomitant increase of secreted a-amylase. …
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Characterization of a novel bioluminescent reporter protein for the non-invasive preclinical imaging of viral infection
… threat [3,4]. The integration of bioluminescent reporter genes into viral genomes presents a cutting-edge approach in understanding how a virus propagates throughout the host. This allows for researchers to non-invasively track the infection's progression from start to finish in laboratory animal …
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Mechanism of transmissible gastroenteritis virus nsp1-mediated translation inhibition
… inhibits the expression of host genes without associating with the 40S ribosomal subunit or inducing the endonucleolytic cleavage of mRNAs. Nsp1 of TGEV strongly inhibits the translation of capped mRNAs, as well as certain internal ribosome entry site (IRES)- containing mRNAs, in …
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Virus-Based Nanoparticles for Tumor Selective Targeting and Oncolysis
… small molecules, tumor antigens, anti-tumor/ reporter genes and also aid in generating tumor specific immunity by rational design.
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Characterization of Negeviruses and insect virome interactions during co-infection in cell culture
… clones of Negeviruses that have fluorescent reporter genes inserted into their genome. Analyzing the growth trends of different Negeviruses in these cell lines allow us to determine which Negevirus isolates can establish infection in the presence of existing or co-infections with other …
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Comparative analyses of aryl hydrocarbon receptor structure and function in marine mammals
… factor, mediating the expression of a suite of genes in response to exposure to DLC and structurally related chemicals. Species-specific differences in AHR structure can affect an organism’s susceptibility to the effects of DLC. The structures and functions of several cetacean AHRs were …
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The Association of the Human Cytomegalovirus Genome with the Cellular Nuclear Matrix
… assay; and 3) activating transcription of reporter genes in stabile transformed cells.</p> <p>The MIE gene was cloned, digested with restriction endonucleases, and the resulting DNA fragments were reacted with nuclear matrices prepared in vitro. Relative to coding region and vector …
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Using a chemical genetics approach to dissect the nitrogen signalling pathway in Arabidopsis
… forms of N accumulate and the expression of many genes associated with nitrate assimilation are repressed. Little is known about the mechanisms involved in this N repression. This project, for the first time, adopts a chemical genetics approach to investigate the feedback regulatory pathway that …
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Aphid Transmission, Replication, and Transcription of BYDV -Pav -Il and Genome Organization of RhPV
… Seven clones activated the expression of the reporter genes, supposedly by interacting with capsid proteins of BYDV-PAV, but complementation tests indicated that all were false positives. Thus, other biochemical and genetic methods may be more successful for the identification of aphid …
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Generation of Transgenic Mice to Evaluate Promoter Activity and Specificity of Two Human Endogenous Retrovirus Long Terminal Repeats = Untersuchungen zur Promotor-Aktivität und -Spezifität von zwei Long Terminal Repeats humaner endogener Retroviren in transgenen Mäusen
… be used to drive the expression of therapeutic genes, delivered by retroviral vector systems, in a safe and efficient manner. The HERV-H-H6 LTR and the HERV-L LTR were chosen for the generation of transgenic mice. Their promoter activity and specificity had prior been tested in a luciferase …
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