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Showing 1 to 20 of 25 for “"reporter gene assays"”.

  1. Characterization of Type IV Pilus System Genes and Their Regulation in Clostridium perfringens

    Murray, Samantha RoseVirginia Tech2017

    … was found to contain Type IV pilus (T4P) system genes within the genomes of all its sequenced strains. T4P are widely used in Gram-negative organisms for aggregation, biofilm formation, adherence, and DNA uptake. Because few examples of T4P-utilizing Gram-positive bacteria are studied to date, we …

    vt Repository record

  2. Modulation der IL-6-abhängigen Signaltransduktion durch die Protein-Tyrosinphosphatase SHP2

    Henke-Gendo, CorneliaPublikationsserver der RWTH Aachen University2002

    … as well as enhanced acute-phase protein gene induction. Further reporter-gene-assays with a dominant negative SHP2 also show a greater inducibility of the examined promotors. Therefore, not only SHP2 itself, also its enzyme activity plays an important role in acute-phase gene regulation.

    aachen Repository record

  3. Role of PI3K and MAPK in Regulating the IL-10-Induced Temporal Transcriptional Program in LPS-Triggered Macrophages

    Dillow, Amanda Fields

    … induces expression of a set of anti-inflammatory genes that act to inhibit expression of TLR-induced pro-inflammatory genes. While many of the IL-10-induced genes can be induced by IL-10 stimulation alone, some others, such as TNIP3, are only IL-10-inducible in the presence of concurrent TLR …

    mo-state Repository record

  4. Regulatory mechanisms involved in the control of <i>CYP6M2</i> gene in insecticide resistant <i>Anopheles gambiae</i> (Diptera: culicidae)

    Mohammed Rabiu, BalarabeAbertay University2014

    Cytochrome P450s, including <i>CYP6M2</i> gene, are involved in the detoxification of permethrin. Some of these genes are regulated by <i>CnCC / dKeap</i> 1 and Spineless / <i>Tango </i>in <i>Drosophila melanogaster</i>. This mechanism is yet to be identified in <i>Anopheles gambiae</i>. Thus, we …

    abertay Repository record

  5. Mutations In Stat3 Associated With Human Hyper Ige Syndrome Enhance Nfκb and Mapk-Mediated Gene Expression

    Greeley, Nathaniel2012

    … mice. Since osteoclast and inflammatory cytokine genes are NFκB targets, these observations indicate a possible deregulation of NFκB signaling in both mice and humans with STAT3-deficiency. Here, we sought to examine the role of STAT3 in the regulation of NFκB-mediated gene expression through …

    uthsc Repository record

  6. Energy Homeostasis During Fasting and Fed States: Foxa2 Regulates Hepatic Amino Acid Uptake

    Lee, Vivian M

    … for arginine-induced insulin secretion. Using gene expression array analysis and semi-quantitative RT-PCR analysis on pancreatic islets from Tcf1 +/+ and Tcf1 -/- mice, we found eight arginine transporters expressed in the pancreatic islets with only two transporters, mNAT3 and CAT3, regulated …

    rockefeller Repository record

  7. Identification of a Minimal Cis-element and Cognate Trans-factors Required for the Regulation of Rac2 Gene Expression during K562 Cell Differentiation

    Muthukrishnan, Rajarajeswari2009

    … the molecular mechanisms regulating Rac2 gene expression during cell differentiation and identification of a minimal cis-element required for the induction of Rac2 gene expression during K562 cell differentiation. The Rho family GTPase Rac2 is expressed in hematopoietic cell lineages and …

    iupui Repository record

  8. Chronic Hypoxia Induces Epigenetic Modifications in the Fetal Rat Heart

    Patterson, Andrew James Grant

    … that events in utero can significantly alter gene expression patterns in heart tissue leading to increase susceptibility to ischemia reperfusion injury in adulthood. The focus of this project was to elucidate the role of chronic hypoxia in the programming of the cardio-protective gene, Protein …

    loma-linda Repository record

  9. Reengineering Estrogen Receptor Hormone Specificity Through Coordinated Changes at Key Contact Sites Between Estrogen Receptor and Estradiol

    Thomas, James AdrianUniversity of Illinois at Urbana-Champaign2015

    … (ER') pairs in quantitative ligand binding and reporter gene assays. The most successful coordinated changes have involved the change of Glu353 to a serine or alanine residue coupled with a change of the 3-OH of estradiol to a carboxymethyl or an ethoxy group, respectively. In these cases, the …

    uiuc Repository record

  10. Regulation of the cyclin-dependent kinase inhibitor p21 by TBX3

    Hare, ShannaghDepartment of Human Biology2013

    … basis for its role in development and oncogenesis because there is a paucity of information regarding its target genes. Of interest to the current study, preliminary evidence suggest that TBX3 may repress the cyclin-dependent kinase inhibitor p21WAF1, which has a role in a myriad of …

    cape-town Repository record

  11. Characterisation of the role of ING5

    Basavarajaiah, PoornimaPublikationsserver der RWTH Aachen University2005

    … This allows mutations to accumulate in other genes and leads to more than 50% of all human cancers. p21WAF1/CIP1 inhibits cyclin E/CDK2 complexes to prevent G1-S progression of cell cycle. Though the cyclin/CDK complexes are required for the orderly progression of the cell cycle, …

    aachen Repository record

  12. Untersuchungen zur TGF-[beta]1-Expression und -Bioverfügbarkeit bei der Leberfibrogenese

    Westhoff, Jens HendrikPublikationsserver der RWTH Aachen University2005

    … of the most important cytokines mediating fibrogenesis in the liver and in other organs. The major portion of it is secreted as latent molecule complexed either only with the “latency associated peptide” (LAP) or with LAP and the “latent TGF-Beta binding protein” (LTBP). Activated hepatic …

    aachen Repository record

  13. Studies on the Maturation of Secreted Quorum Sensing Peptides That Regulates S. Aureus Virulence

    Johnson, Jeffrey Grant

    <p>The accessory gene regulator (agr) locus in the commensal human pathogen, Staphylococcus aureus, is a two-promoter operon with allelic variability that encodes a quorum sensing circuit involved in regulating virulence in the bacterium. Secretion of unique autoinducing peptides (AIPs) and …

    rockefeller Repository record

  14. Characterisation of oestrogenic properties of Isoflavones derived from Millettia griffoniana Baill.: - Molecular mode of action and tissue selectivity

    Ketcha Wanda, Germain Jean MagloireTechnische Universität Dresden

    … oestrogen receptor alpha (ERα) dependent assays, namely a recombinant yeast assay, a reporter gene assay based on stably transfected MCF-7 cells (MVLN cells) and the induction of alkaline phosphatase in Ishikawa cells. The oestrogenic activities of isoflavones from Millettia griffoniana …

    qucosa-diss

  15. Measurement and risk assessment of endocrine disruptors present in sport supplements

    Plotan, MonikaQueen's University Belfast2011

    … (MMV-Luc)and androgen (TARM-Luc) responsive reporter gene assays (RGAs), previously developed by Willemsen et al. (2004), in the screening of sport supplements forendocrine disrupters (EDs). This included the development of a general extraction procedure suitable for a range of sport …

    qu-belfast Repository record

  16. Estrogenic and androgenic endocrine disrupting activity and risk assessment of phytoestrogens in Kenyan soya-based weaning flours

    Ngoda, PeninahQueen's University Belfast2012

    … widespread in Kenya. Estrogenic and androgenic reporter gene assays (using MMV-Luc and TARM-Luc cell lines respectively) were used to characterise the transcription activity of ten PEs (apigenin, daidzein, daidzin, genistein, genistin, enterodiol, equol, formononetin, glycitein and matairesinol) …

    qu-belfast Repository record

  17. Investigations into the vaccinia virus immunomodulatory proteins C4 and C16

    Scutts, Simon RobertUniversity of Cambridge2017

    … NF-κB signalling by an unknown mechanism. Reporter gene assays showed that C16 also suppresses NF-κB activity and, intriguingly, this was carried out by both the N and C termini. C16 acts at or downstream of p65 and the N terminus of C16 associated with p65 independently of PHD2-binding. …

    cambridge Repository record

  18. Molekulares Targeting von Hepatischen Sternzellen zur Therapie der Leberfibrose

    Benner, SusannePublikationsserver der RWTH Aachen University2007

    … in activated HSC specifically. Therefore, the genes for the pro-apoptotic proteins p53, Bax and HSV1tk were cloned under the transcriptional control of a human CSRP2-promoter fragment. Those gene cassettes have been placed in replication deficient adenoviruses to infect HSC. In different assays …

    aachen Repository record

  19. Investigations into the role of Toll-Like Receptors in the modulation of the inflammatory response during Osteoarthritis

    Maratha, AshwiniNational University of Ireland Maynooth2012

    … are sentinel cells that contribute to OA pathogenesis, through secretion of various catabolic and pro-inflammatory mediators, though the downstream stimuli which initiate and propagate the inflammatory pathway remain poorly defined. Activation of the innate immune Toll-Like Receptors (TLRs) …

    maynooth Repository record

  20. IMPACT OF CONVENTIONAL AND PRECISION CATTLE MANURE APPLICATION METHODS ON HORMONE CONCENTRATIONS AND ACTIVITIES IN SURFACE WATER AND SOIL

    Thresher, JocelynUniversity of Saskatchewan2023

    … viability. Intensive animal production can generate vast quantities of animal manure that is concentrated within relatively limited areas. Managing manure is challenging, but management often involves applying manure to agricultural lands. Applying manure to land recycles valuable nutrients …

    sask Repository record

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