Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"reporter gene assay"”.
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Studying the Specificity of hPXR Antagonists Using a Panel of Cell-based Assays
… the transcription of a large network of genes including cytochrome P450 (CYP) and Mdr1 which play critical roles in chemicals metabolism and transportation. Induction of CYPs contributes to adverse drug-drug interactions. Non-toxic, PXR-specific antagonists will be valuable in attenuating …
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Vitamin E : elucidation of the mechanism of side chain degradation and gene regulatory functions
… like CYP3A4, a pregnane X receptor (PXR)-based reporter gene assay was chosen. PXR is a nuclear receptor which regulates the transcription of genes, e.g., CYP3A4, by binding to specific DNA response elements. And indeed, as shown here, vitamin E is able to influence the expression of CYP3A via …
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Controlling Gene Expression with Synthetic Molecules
Aberrant gene expression patterns have been implicated in several pathological states. Synthetic molecules capable of functionally mimicking native transcription factors and regulating gene expression in a specific and predictable manner may represent a new paradigm in drug development. Native …
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New methodologies for studying the biological activity of platinum complexes
… of which were recognized by HMG1 in a gel shift assay. In order to identify the platinum complex(es) responsible for this behavior, a sublibrary synthesis and screening approach was employed. After three iterations of sublibrary synthesis and screening, [Pt(lysine)C12] (Kplatin) was identified as …
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Identification of motifs in the tribbles kinase required for substrate interactions
The gene tribbles encodes an atypical kinase that is evolutionarily conserved with important roles in regulation of cell signaling. In Drosophila melanogaster Tribbles (Trbl) directs the proteasomal degradation of the C/EBP transcription factor Slbo (slow border cells) to regulate cell migration in …
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Characterisation of oestrogenic properties of Isoflavones derived from Millettia griffoniana Baill.: - Molecular mode of action and tissue selectivity
… oestrogen receptor alpha (ERα) dependent assays, namely a recombinant yeast assay, a reporter gene assay based on stably transfected MCF-7 cells (MVLN cells) and the induction of alkaline phosphatase in Ishikawa cells. The oestrogenic activities of isoflavones from Millettia griffoniana …
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The effect of different sample preparatory protocols on the induction of the aryl hydrocarbon receptor (AhR) in the H4IIE–luc reporter gene bio–assay
… of dioxins using the H4IIE-luc-reporter gene assay. The protocols evaluated were either Soxhlet or pressurised liquid extraction (PLE) combined with a manual acid digestion, gel permeation chromatography (GPC) and Florisil fractionation clean-up procedure as well as the automated …
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Part I: Steroid Receptor CO-Activator 3 (SRC-3) Expression in Lung Cancer and its Role in Regulating Cancer Cell Survival and Proliferation; Part II: Development of Phosphospecific Peptoid Ligand
… breast cancer, however, its role in lung tumorigenesis is unknown. We found that SRC-3 is over-expressed in 27% of the NSCLC patients, and SRC-3 high expression correlates with poor disease-free survival and overall survival. We also studied DNA copy number, mRNA and protein expression of SRC-3 …
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Stress and Growth Related Keratinocyte Pathways
… response is upregulation of immediate early genes that initiate and coordinate subsequent cellular events. IEX-1 is a novel immediate early gene that has been shown to be induced by gamma irradiation, phorbol ester treatment and ultraviolet irradiation. Goal of this thesis was to more …
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Glucocorticoid receptor signalling and the effect of interleukin 1 beta on glucocorticoid mediated gene expression in intestinal epithelial cell lines Caco-2 and IEC-6
… GR. Its quantity was determined by radiobinding assay in both cell lines. The regulatory (activating and repressing) effects of dexamethasone on gene transcription in presence or absence of IL-1b were analyzed by reporter gene assay using either glucocorticoid receptor- (pGRE-SEAP) or nuclear …
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Posttranscriptional and posttranslational regulation of virulence in erwinia amylovora
… virulence regulation. Mutation of the lon gene caused the amylovoran overproduction, the increased T3SS expression and the non-motile phenotype. In the absence of Lon, abundance and stability of the HrpS/HrpA and RcsA proteins were significantly increased, and the resulting accumulation of …
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Characterisation of the HIV inhibitory activity of vaginal lactobacilli isolates from young South African women at high risk of HIV acquisition
… HIV infectivity was evaluated using a Luciferase Reporter Gene Assay in TZM-BL cells. Lactobacilli isolated from women with non-optimal microbiota produced less lactic acid and induced greater inflammatory cytokine production than those from women with optimal microbiota, with IL-6, IL-8, IL-1a, …
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Entwicklung eines Dual-Luciferase-Reportergen-Assays zum Nachweis der Induktion antioxidativer Enzyme durch Nahrungsbestandteile
… (z. B. Herz-Kreislauf-Erkrankungen, Neurodegeneration, Atherosklerose) vorzubeugen. Ausgehend davon wurde in der vorliegenden Arbeit der Dual-Luciferase-Reportergen-(DLR)-Assay zum Nachweis der Induktion der antioxidativen Enzyme Katalase (CAT), zytosolische Glutathion-Peroxidase (GPX1) und …
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Investigating persistent organic pollutants as endocrine disruptors of androgen receptor signalling
Man-made chemicals are a part of everyday life and may mimic hormonal signalling in wildlife and humans. These exogenous mixtures or substances are called endocrine disrupting chemicals and may lead to adverse health effects in those exposed. Persistent organic pollutants (POPs) are a class of …
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IMPACT OF CONVENTIONAL AND PRECISION CATTLE MANURE APPLICATION METHODS ON HORMONE CONCENTRATIONS AND ACTIVITIES IN SURFACE WATER AND SOIL
… viability. Intensive animal production can generate vast quantities of animal manure that is concentrated within relatively limited areas. Managing manure is challenging, but management often involves applying manure to agricultural lands. Applying manure to land recycles valuable nutrients …
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Characterizing the Binding Potential, Activity, and Bioaccessibility of Peroxisome Proliferator Activated Receptor Gamma (PPARγ) Ligands in Indoor Dust
… some environmental contaminants may alter adipogenesis, resulting in accumulation of adipocytes, and often significant weight gain. Thus these types of contaminants are often referred to as obesogens. Many of these contaminants act via the activation (i.e. agonism) of the peroxisome proliferator …