Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 9 of 9 for “"reporter enzyme"”.
-
Secondary Level Screening of Chlamydomonas Reinhardtii Mutants Defective in Circadian Gene Expression
… gene expression. In a previous study, the reporter gene ARS2 encoding the arylsulfatase enzyme was fused to the promoter of the circadian-regulated CABII-1 gene and transformed into the Chlamydomonas nucleus. The ble marker was introduced into the genome of this transformant via insertional …
-
Construction of an efficient secretion system for recombinant proteins in Bacillus subtilis
… in Bacillus subtilis using an a-amylase as a reporter enzyme, which is secreted into the medium using the Sec pathway. Its gene amyQ was fused to an IPTG-inducible promoter. It turned out that increasing amounts of IPTG did not result in a concomitant increase of secreted a-amylase. …
-
Transcriptomics-Guided Biodiscovery of Peroxidases from Macroalgae
Peroxidases are versatile enzymes with applications in laboratory analytics and industrial processes. Despite their relevance, many applications rely on a few well-characterized terrestrial enzymes, particularly horseradish peroxidase (HRP), whose complex post-translational modifications hinder …
-
Integrated Microfluidic System for Digital Detection of Extracellular Vesicles
… chip achieves the EV quantification by digital Enzyme-Linked Immunosorbent Assay (ELISA) and enzymatic signal amplification. Specific areas of glass slide beneath the detection area of the chip coated with anti-CD81 antibodies are used to capture EVs. A cocktail mixture of biotin-labeled …
-
Identification and characterization of components that overcome secretion limitations of the yeast Pichia pastoris
… was randomly disrupted through restriction enzyme-mediated integration of an antibiotic resistance marker. Supersecretion mutants were identified by their ability to secrete <strong>β</strong>-galactosidase, a reporter enzyme not natively secreted by P. pastoris. Sixteen …
-
Engineered heme peroxidases as genetically encoded probes with diverse applications in cell biology
… Horseradish peroxidase (HRP), a multifunctional reporter enzyme widely used in bioanalytical assays, has been utilized for EM because of its ability to generate electron-dense deposits. In addition, HRP produces extremely sensitive chemiluminescent, colorimetric, and fluorescent readouts and has …
-
Identification of Regulatory Binding Sites and Corresponding Transcription Factors Involved in the Developmental Control of 5'-nucleotidase Expression in Dictyostelium discoideum
… designed and fused to the luciferase and lacZ reporter genes, and reporter enzyme activity was measured in cells from the slug stage of development. The results from these experiments enabled the identification of a 250 bp region of the promoter, which was used as a template for subsequent …
-
Esterase 2-oligodeoxynucleotide conjugates as enzyme reporter for electrochemical detection of DNA and identification of bacterial species
… of nucleic acids detection, a suitable enzyme reporter is expected. Esterase 2 (EST2) from Alicyclobacillus acidocaldarius was introduced and mutated to have an accessible cysteine residue at 118th codon. This esterase was purified by a single-step affinity chromatography with …
-
Biochemical And Structural Characterization Of The Core Subunits Of Gpi Transamidase
… is a complicated, membrane-bound, multi-subunit enzyme that catalyzes an essential post-translational modification. This enzyme attaches GPI anchors to the C-termini of various proteins that contain a proper GPI-T signal sequence. Gpi8, Gaa1, Gpi16, Gpi17 and Gab1 are the five known subunits that …