Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 79 for “"reporter assay"”.
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Development of Hif-1Α/Hif-1Β Heterodimerization Inhibitors Using A Novel Bioluminescence Reporter Assay System For In Vitro High Throughput Screening and In Vivo Imaging
<p>Tumor growth often outpaces its vascularization, leading to development of a hypoxic tumor microenvironment. In response, an intracellular hypoxia survival pathway is initiated by heterodimerization of hypoxia-inducible factor (HIF)-1α and HIF-1β, which subsequently upregulates the expression of …
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Validation-based insertional mutagenesis (VBIM) technology identifies adenomatous polypossis coli (APC) like protein (ALP) as a novel negative regulator of NF-κB
… of ALP led to decreased NF-κB activity by κB reporter assay and electrophoresis mobility gel shift assay (EMSA). The current project aims to further evaluate the role of ALP in the regulation of NF-κB signaling in CRC cells. We found that overexpression of ALP in human CRC HT29 cells greatly …
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Characterization of Genomic Variation Related to Hair and Skin Phenotypes in the Khoesan Speakers of Southern Africa
… rs7307165, and conducted a dual luciferase reporter assay to determine if rs7307165 influenced gene expression. Results showed rs7307165 significantly affected enhancer activity in keratinocytes for KRT78. Missense mutations in KRT74 and KRT71 were also identified and appear to be compelling …
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DESIGN AND INVESTIGATION OF DUMBBELL-SHAPED DNA MINIMAL VECTORS FOR CRISPR/CAS9 EDITING
… and Southeast Asian Ovalocytosis, and a dual reporter assay, we demonstrated that dumbbell vectors offer a viable alternative to plasmids for delivery of the CRISPR/Cas9 gene editing tool. Our analyses of sgRNA structure revealed that unstructured CRISPR RNA (crRNA) domains in sgRNA exhibited …
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MicroRNA as Regulator of the Th17 Inflammation in Behcet's disease
… flow cytometry, enzyme-linked immunosorbent assay (ELISA), and real-time polymerase chain reaction (PCR) in peripheral blood mononuclear cells (PBMCs) with or without Th17 differentiation condition. The expression of miR-155 was also investigated using real-time PCR in PBMCs and isolated CD4+ …
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Pathogenicity and selective constraint in the non-coding genome
… Finally, I conducted a pilot experiment to assay >50,000 different non-coding variants across more than 700 different non-coding elements, including variants observed in patients with developmental disorders in a massively parallel reporter assay (MPRA) and collaborated on an assessment of …
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Biochemical and Molecular Characterizations of a Novel DNA Repair Enzyme, CPD -Photolyase, and Late Promoters of Fowlpox Virus
… promoters were attempted using a lacZ gene based reporter assay. The transcriptional element of ORF P190 appeared to be the strongest. In contrast, the promoter associated with the ORF, P180, was the weakest. Interestingly, a novel bi-directional promoter controlling the expression of ORFs 130 and …
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Promoter Polymorphisms of Cysteinyl Leukotriene Receptor 1 (CysLTR1) Influence the Effect of Leukotriene Modifier on Transcriptional Regulation of the CysLTR1 Gene Induced by IL-4
… genetic polymorphism were examined by luciferase reporter assay. Transcriptional regulation of endogeneous CysLTR1 gene induced by IL-4 was investigated using lung carcinoma cell line, A549 cells, by real time PCR and flowcytometry. Effect of MK-571 on the transcriptional induction of CysLTR1 by …
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Yeast Ataxin-2, A Protein with Many Friends: Identification of a Novel Role for Pbp1 in Mitochondrial Biogenesis
… respiration. Utilizing genetics and biochemical assays, I discovered that loss of Pbp1 leads to decreased Puf3-specific mitochondrial proteins, suggesting a functional relationship between Pbp1 and Puf3. I also found that Pbp1 stabilizes and promotes Puf3-target mRNAs in respiratory conditions …
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P107 negatively regulates the neural precursor pool by repressing Hes1 transcription
… repress Hes1 promoter activity in the luciferase reporter assay, and finally, using the primary neurosphere assay we showed that removal of Hes1 from p107-/- neurospheres restores the number of neurosphere forming cells to wildtype levels. Our results suggest that p107 represses Hes1 transcription …
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Exploring Molecular Mechanisms Controlling Skin Homeostasis and Hair Growth. MicroRNAs in Hair-cycle-Dependent Gene Regulation, Hair Growth and Associated Tissue Remodelling.
… as well as selected keratin genes. Luciferase reporter assay revealed that Krt16, Krt17, Dlx3, and Fgf10 serve as direct miR-31 targets. In addition, miR-214 was identified as a potent inhibitor of the Wnt signalling pathway in the keratinocytes. Mutually exclusive expression patterns of …
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Functional Analysis of KAP1 Requirements for HIV-1 Transcription and Latency Reactivation
… domain deletion and mutagenesis in a cell-based reporter assay, I genetically defined the RING finger domain and an Intrinsically Disordered Region as key activating features. However, KAP1 acute loss in a physiological relevant model, reveals limitations compared to chronic silencing of KAP1 in …
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The HIV-1 entry inhibition capabilities of Isoflavones
… the generation of IC50 values using a luciferase reporter assay. Evidence for potential entry-inhibiting activity was generated through time-of-addition studies and the potential drug targets were hypothesised using computational docking studies. All the Isoflavones were shown to inhibit HIV …
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Significant Association of ADORA3 Genetic Polymorphisms with Aspirin-Intolerant Urticaria in a Korean Population
… in ADORA3 were analyzed by luciferase reporter assay, electrophoretic mobility shift assay (EMSA), and real-time PCR for the mRNA expression in PBMC of AIU patients. RESULTS: The haplotype [T-1050C-564] frequency of ADORA3 gene showed significantly higher in AIAU group than in AICU and …
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Development of an Interferon Bioassay and Primitive Endoderm Cell Lines to Study Lineage Specification During Early Bovine Embryogenesis
… interferon stimulatory response element (ISRE)-reporter assay was created, and provides adequate quantification to measure IFNT. Additionally, it has a shorter completion time than previous bioassays, and does not require the use of a live virus. The second study describes the development of a …
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Systematically and proactively testing variant effects for AIRE and SOD1
… comes from (often cell-based) functional assays of variant impacts. One-at-a-time variant functional assays are the current standard, where results often lag months or years after a rare variant has first been detected in a patient. Variant effect ‘maps’ are ‘look-up tables’ which have …
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Biological and Functional Consequences of Single Nucleotide Polymorphisms of the O6-Methylguanine-DNA-Methyltransferase Gene
… In addition, we used the luciferase reporter assay to determine effects of SNPs in the promoter/enhancer region of MGMT on promoter activity. Coding SNPs had a marginal effect on macrolesion damage after exposure to alkylating agents. However a strong effect of coding SNPs on …
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Defining the mechanism(s) of Hepatitis C virus (HCV) entry
… for high-throughput screening, most cell-based reporter assays rely on the secretion of serine alkaline phosphatase and thus do not allow the selection of HCV infected cells, or the screening of patient samples to identify cell culture infectious viral strains. We aimed to develop a cell-based …
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Evaluation of the potential functions of Avian paramyxovirus Accessory proteins
… tested for IFN antagonism by a dual luciferase reporter assay. Our results showed that APMV-3V acts as IFN antagonist by blocking IFN induction and thereby may play an important role in the evasion of innate immunity.
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Investigating microRNAs during cardiac development in the chick
… at least one of these three miRNAs by luciferase reporter assay, including genes in the Wnt signalling pathway which is essential at specific stages of heart development7. The roles of miR-1, known to be indispensable for heart formation and function, were also studied in the developing chick …
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