Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 1252 for “"reporter"”.
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Photoacoustic Tomography of Diabetes and Reporter Genes
<p>Photoacoustic Tomography (PAT) is a hybrid technology which combines light absorption contrast with ultrasonic detection to achieve deep imaging of normally obscured absorbing molecules in tissue. PAT's inherent background free, super-depth imaging capability makes it particularly well suited …
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The Application of a Reporter Group to α-Chymotrypsin
<p>A reporter group method was developed in order to obtain information about small changes in the environment at specific positions in protein molecules. In this method one environmentally sensitive group is introduced into a specific position in the protein so that small changes induced by …
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Photoacoustic Reporter Gene Imaging And Optical Coherence Computed Tomography
… mice and rats, using a chromogenic lacZ/X-gal reporter gene system. We demonstrated the expression of the lacZ reporter gene can be detected by PAI as deep as 5 cm inside tissue. In addition, we showcased that PAI could follow gene expression from the microscopic to the macroscopic level. This …
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A Dual-GFP Reporter System for Studying Bi-Directional Promotion
… transcription. Green Fluorescent Protein (GFP) reporters may be suitable for simultaneous measurement in a dualreporter system. GFP variants have similar half-lives, sensitivity for detection, and distinct excitation and emission wavelengths. The GFP variant, ECFP and EYFP, were chosen as …
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Rgs16 is a Pancreatic Reporter of Chronic Hyperglycemia in Diabetes
… and Rgs16::GFP PDAC culture and diabetic reporter mice are beneficial resources to identify ligands that stimulate beta cell expansion without promoting cancer.
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Haploid reporter screens aimed at identification of NF-kappaB regulators
… screens through the use of transcriptional reporters and developed human haploid reporter screens for the specific purpose of identifying regulators of NF-kB. To identify constitutive inhibitors of NF-kB, I utilized a NF-KBblasticidin S resistance gene (BSR) reporter and identified CYLD, a …
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The Tele-Reporter : a collaborative interface for goal orientation teledirection
Thesis (M.Eng.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2002.
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Surface Displayed SNAP as a New Reporter in Synthetic Biology
… an extracellular synthetic green fluorescent reporter, SNAP-Cell® 505-Star, simultaneously with an intracellular red fluorescent protein, mCherry. Moreover, we have shown that our construct enables cells to selectively bond to BG-conjugated magnetic beads. As a result, we have demonstrated …
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A Two-colour Reporter Screen and Application to Cell Cycle Transcription
… In this Thesis, I describe a two-color GFP-RFP reporter system I developed to assess the consequences of genetic perturbations on a promoter of interest. The dual-reporter system is compatible with the synthetic genetic array methodology, an approach that enables marked genetic elements to be …
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Evaluating luteinizing hormone receptor signaling using the cyclic AMP reporter ICUE3
… These studies used ICUE3, an EPAC-based reporter molecule for cAMP in cells. ICUE3 is an engineered molecule that contains a FRET donor and acceptor pair as well as a membrane-targeting sequence. FRET occurs when the donor fluorophore in an excited electronic state transfers its …
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Methods for the Genetic Stabilization of Reporter Flaviviruses and their Applications
Infectious reporter flaviviruses have been reported since 2003, and they have the potential to be utilized in diverse scientific areas such as investigating viral pathology, conducting high-throughput drug screens, running rapid serum neutralization tests, and diagnosing disease. Despite the value …
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Methods for the Genetic Stabilization of Reporter Flaviviruses and their Applications
Infectious reporter flaviviruses have been reported since 2003, and they have the potential to be utilized in diverse scientific areas such as investigating viral pathology, conducting high-throughput drug screens, running rapid serum neutralization tests, and diagnosing disease. Despite the value …
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Ruolo biologico delle conformazioni dei biopolimeri. Le porfirine come reporter specifici
… metodi biofisici e impiegando le porfirine come reporter specifici. Inoltre, ho studiato come la stabilità dei microRNA influenzi l'attività biologica e ho dimostrato il ruolo delle porfirine nell'interazione con sequenze di miRNA e RNA telomerico. Abbiamo anche analizzato sequenze di DNA …
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"The ""National Catholic Reporter"": Communications and change in a turbulent era"
Made available in DSpace on 2015-05-12T22:32:27Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 7310032.PDF: 21148871 bytes, checksum: bc37f77cb60ed1a2beb4aa9703b5c1b9 (MD5) Previous issue date: 1972
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Predictive Reporter System for Investigating Dose Dependency in Oncogene-Induced Senescence
… potential utility of our proposed ‘predictive reporter’ system in addressing gene dose-related biological questions. RNA sequencing of sorted subpopulations demonstrates the effect of RAS dose on gene expression, where between-subpopulation differences suggests that this sorting strategy may …
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Investigating luteinizing hormone receptor signal transduction using the cyclic AMP reporter ICUE1
… biochemical methods. ICUEl is an Epac-based cAMP reporter which undergoes conformational changes after binding cAMP. Unlike traditional biochemical assays, ICUEl combined with FRET techniques is capable of real-time monitoring of cAMP levels in individual cells. In this project, Epac reporters …
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Identification of Anti-inflammatory and Antioxidant Properties of MangostinXanthones in Adipocyte Reporter Assays
… factor2 promoter (<i>Nrf2</i>-P)/YFP fluorescent reporters. We hypothesized that fluorescent signals directly correlated to inflammatory, adipogenic, and antioxidant status in adipocytes, respectively. Two polyphenolic xanthones, α-mangostin (α-MG) and γ-mangostin (γ-MG) from mangosteen fruit, …
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