Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 40 for “"replicon"”.
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Molecular and genetic analysis of RepA from the P307 RepFIB replicon
… control system of the P307 plasmid RepFIB replicon. The basic replicon occupies ≈ 1.6kb of DNA and contains a single large open reading frame (repA) flanked on either side by a series DNA repeat elements. The organisational structure of fie replicon has placed RepFIB into the Step function …
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Optimisation of the mycobacterial replicon of an E. coli-mycobacterial shuttle vector
… aimed to investigate whether the mycobacterial replicon may also play a role. The common mycobacterial replicon in episomal E. coli-mycobacterial shuttle vectors contains the truncated rap gene, encoding an auxiliary replication factor, and the repA and repB genes which code for essential …
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Ancestral Genome Reconstruction in Bacteria
… methods developed so far only focus on gene or replicon sequences instead of whole genomes. They rely largely on either detailed modeling of evolutionary events or edit distance computation, both of which can be computationally prohibitive for large data sets. Hence, most of these methods can …
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Identification of host cellular factors that regulate human norovirus replication
… is limited. In this study, we used the virus replicon system in human gastric tumour (HGT) cells to determine host factors involved in human norovirus replication, and to analyse the adaptive changes required to support its long-term replication in cell culture. First, using a genome-wide …
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The interaction of hepatitis C virus and intracellular lipid metabolism
… lipid metabolism. Treatment of HCV genotype 1b replicon containing cells with cerulenin, which inhibits fatty acid synthase, reduced replication of HCV RNA in a dose dependent manner (Su et al., 2002). Polyunsaturated fatty acids (PUFAs) have recently been shown to inhibit replication of a …
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An investigation of the replication of the broad-host-range plasmid pTF-FC2
… this fragment was used to identify the minimal replicon, the vegetative origin of replication (orzV) and the areas determining plasmid incompatibility. The copy number of pTF-FC2 in E. coli was estimated at 12 - 15 copies per chromosome and a deletion plasmid was identi.fied which replicated at …
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Viral and Host Genetic Determinants of Hepatitis C Virus Persistence and Interferon Resistance
… therapy. The development of the HCV RNA replicon system has allowed the study of persistent HCV RNA replication in tissue culture. We evaluated HCV/IFN interactions within a long-term culture system of Huh7 cell lines harboring different variants of an HCV genotype 1b subgenomic RNA …
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HPV pseudovirion production in plants
… vectors demonstrated the formation of a replicon, and autonomous replication, in Nicotiana benthamiana plants. Each of these vectors were co-infiltrated with the non-replicating transient plant expression constructs pTRAc-hL1 and pTRAc-hL2, which encode human-codon optimised forms of …
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Evaluation of arthropod-borne virus recombination potential and phenotype: In vitro and in vivo analysis of flavivirus and alphavirus model systems
… were used to generate CHIKV, SINV, and YFV 17D replicon/defective helper systems possessing trans-complementary sequences necessary and sufficient for the generation of full length viable recombinant viruses following transfection, replication, and purification in cell culture.\r\n\r\n …
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Control of the Interferon Regulatory Factor - 3 Antiviral Pathway by The Hepatitis C Virus Ns3/4a Protease
… of the antiviral response. Using the cell-based replicon system, HCV was found to inhibit the activation of critical intracellular signaling pathways. Our studies identified the NS3/4A protein as an interferon regulatory factor 3 (IRF-3) and NF-<small>K</small>B antagonist and further mapped this …
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GCD10 and GCD14 Proteins Facilitate Tomato Bushy Stunt Viral (TBSV) RNA Replication in Saccharomyces Cerevisiae
… RNA replication, the replication of a TBSV RNA replicon was analyzed in wild type, GCD10 knockdown and GCD14 knockout yeast strains. Reduction of GCD10 protein and deletion of GCD14 protein in the yeast strains significantly inhibited TBSV subviral defective interfering (DI) RNA replication, …
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Control of the Interferon Regulatory Factor - 3 Antiviral Pathway by The Hepatitis C Virus Ns3/4a Protease
… of the antiviral response. Using the cell-based replicon system, HCV was found to inhibit the activation of critical intracellular signaling pathways. Our studies identified the NS3/4A protein as an interferon regulatory factor 3 (IRF-3) and NF-<small>K</small>B antagonist and further mapped this …
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The function of the hepatitis C virus p7 protein
… both HCV infection and expression of p7 from replicon RNAs reduced the number of highly acidic (pH<5) vesicles and increased lysosomal pH from 4.5 to 6.0. These effects were not present in uninfected cells, sub-genomic replicon cells not expressing p7, or cells electroporated with viral RNA …
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Molecular Genetic Characterization of the Mating Pair Formation and Vegetative Replication Regions on the Agrobacterium Tumefaciens Ti Plasmid pTiC58
… Rhizobiaceae evolved from a basic rep-tra-trb replicon. Other loci encoding various functions were incorporated into this hypothetical replicon in a modular fashion to give each plasmid its distinct characteristics.
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Understanding the Role of the Hypervariable Region in the Open Reading Frame 1 of the Hepatitis E virus in Viral Replication
… 747 to 761 in the HVR of a sub-genomic GFP HEV replicon. Expression of enhanced green fluorescent protein by the mutant hHVRd2 confirmed the dispensability of amino acid residues 747-761 of the HVR. To confirm our in vitro results, specific-pathogen-free (SPF) chickens were intra-hepatically …
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Investigations into the use of maize streak virus as a gene vector
… a gene vector. These involved testing MSV-based replicons in transgenic cell lines, in transient expression assays in maize cells and in an infectious gene expression system in plants. MSV vectors which contained three different versions of a bar (bialaphos resistance) expression cassette in …
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Geobacillus genties bakterijų genetinės transformacijos sistemos kūrimas /
… for construction of new shuttle vectors. The replicon of this plasmid ensures high segregational stability and provides high copy number to it bearing vector. Further, the electrotransformation protocol for the transfer of the vector DNA to Geobacillus stearothermophilus NUB3621R cells was …
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Development of genetic transformation system for bacteria of the genus Geobacillus /
… for construction of new shuttle vectors. The replicon of this plasmid ensures high segregational stability and provides high copy number to it bearing vector. Further, the electrotransformation protocol for the transfer of the vector DNA to Geobacillus stearothermophilus NUB3621R cells was …
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