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Showing 1 to 15 of 15 for “"replication protein A"”.
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Cloning and Characterization of Replication Protein A from Dictyostelium discoideum
The gene encoding the Dictyostelium replication protein A large subunit (DdRPA1) has been cloned by screening of an EcoR I partial genomic library and a Hind III genomic sub-library. The complete nucleotide sequence, including the promoter region of the gene has been obtained by sequencing. Though …
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Novel Roles of Replication Protein A Phosphorylation in Cellular Response to DNA Damage
<p>Human replication protein A (RPA) is an eukaryotic single-stranded DNA binding protein directly involved in a variety of DNA metabolic pathways including replication, recombination, DNA damage checkpoints and signaling, as well as all DNA repair pathways. This project presents 2 novel roles of …
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1, Structural and Functional Studies of Human Replication Protein A; 2 DNA Damage Responses and DNA Repair Defects in Laminopathy-Based Premature Aging.
… of DNA repair and DNA damage checkpoints. Human replication protein A (RPA), a single-stranded DNA (ssDNA) binding protein, is essential for almost all DNA metabolic pathways. However, the role of RPA in nucleotide excision repair (NER), a DNA repair pathway for removing bulky DNA lesions, …
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Functional Analysis of Novel Replication Protein a Homologs in the Euryarchaeota: Implications in the Evolution of Single -Stranded DNA Binding Proteins Across the Three Domains of Life
Single-stranded DNA binding proteins (SSB) play an essential role in all cellular DNA transactions including DNA replication, repair, and recombination. At the replication fork, SSBs serve to stabilize and protect the transient intermediate ssDNA, which is generated by a replicative helicase. …
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Footprint Analysis of the Transcriptional Control of Glycogen Phosphorylase 2 in Dictyostelium Discoideum
… to template, nonspecific competitor, source of protein extract and DNase I digestion. Using an endlabeled fragment containing seven repeated sequences (3 TA boxes [TAATTATA], 2 TAG boxes [TAAAAATGGT] and 2 C boxes [ACCCACT]), purified replication protein A and several developmental nuclear …
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The effect of single-stranded DNA binding protein RPA2 on XPD helicase processivity
Understanding how proteins work together to perform vital cellular functions, such as replicating and repairing DNA, not only extends our understanding of fundamental biology, but can also lead to important medical interventions. As a necessary first step to understanding larger systems, we focus …
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The Function and Mechanism of RNA Interference in Neurospora
… complex (RISC), in which an Argonaute family protein, guided by a siRNA, mediates the cleavage of homologous RNAs. In the filamentous fungus Neurospora, we show that dsRNA not only trigger RNAi, it also transcriptionally activates several key components of RNAi pathway, including qde-2 (an …
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Structural and Biochemical Investigation of the Molecular Mechanisms of DNA Response and Repair in Humans and <em>Escherichia coli</em>.
… evolved to recognize and eliminate DNA damage. Replication protein A (RPA), the eukaryotic single-stranded DNA (ssDNA) binding protein, is a required factor for all major DNA metabolisms. Although much work has been done to elucidate the nature of the interaction between RPA and ssDNA currently …
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Molecular, Biochemical, and Evolutionary Analysis of Single-Stranded Dna-Binding Proteins From the Mesophilic Archaeon Methanosarcina Acetivorans
"One of the critical proteins involved in DNA replication across the three domains of life is single-stranded DNA-binding protein. At the replication fork, these proteins bind to stabilize and protect transiently exposed ssDNA from nucleases during DNA transactions. The ssDNA-binding protein is …
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Replication-associated base excision repair Of oxidized bases in the mammalian genome
… as substrates and is upregulated at the mRNA and protein level during S-phase. NEIL1 also demonstrates the unique able to excise base lesions from forked or single-stranded DNA substrates that mimic intermediates generated during DNA replication. This suggests a direct linkage of NEIL1’s repair …
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Characterization of SsoSSB, Sso1450, Sso2001 Proteins and Analysis of CRISPR and cas Genes from Sulfolobus solfataricus P2
… a newly discovered single-stranded binding (SSB) protein from Sso P2 and the computational and experimental analyses of clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR-associated (cas) genes, respectively. The DNA-binding properties of SsoSSB, the organization of the …
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Characterisation of factors that regulate homologous recombination and antigenic variation in Trypanosoma brucei
… involves switches in the Variant Surface Glycoprotein (VSG) expressed on the cell surface. Such reactions can occur at very high rates (~10-3 switches/cell/generation) and occur primarily by the recombination of VSG genes, selected from an enormous silent archive, into specialised expression …
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Single-molecule optical trapping studies of the regulation mechanisms of XPD helicase
… DNA integrity is essential, as repair, replication, and protective networks ensure that the genetic code is faithfully preserved and transmitted, allowing organisms to thrive and pass on their legacy. Helicases, as molecular motors, play a critical role in this maintenance by unwinding …
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Visualization, Kinetics, and Thermodynamics of DNA-Protein Interactions
… and structural information about DNA–protein interactions. Furthermore, the micrographs obtained by cryo-TEM were compared to AFM images taken in a previous work. The main goal of this work was to investigate the influence of surfaces on DNA–protein interactions, therefore the methods …
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Distinct functions of POT1 at telomeres.
Telomeres are nucleoprotein complexes that constitute the ends of eukaryotic chromosomes. Telomeres differentiate the end of the chromosome from sites of DNA damage and control cellular replicative potential. The loss of function of telomeres results in several biological consequences. First, …