Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 30 for “"repeat proteins"”.
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Smart Nanomaterials from Repeat Proteins and Amyloid Fibrils
… materials are amyloid fibrils and tandem repeat proteins. Amyloid fibrils are exceptionally strong, tough, highly-ordered structures that self-assemble from a wide range of simple building blocks. Meanwhile, tandem repeat proteins are a class of proteins that act as scaffolds to mediate …
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Study and engineering of nucleic acid-binding repeat proteins
… biology are governed by interactions between proteins and nucleic acids. Some of the nucleic acid-binding proteins have evolved repetitive structures and a modular sequence recognition mode. In particular, DNA-binding transcription activator-like effectors (TALE) and RNA-binding Pumilio/fem-3 …
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Inhibiting Protein-Protein Interactions using Rationally-designed Repeat Proteins
… of consensus-designed tetratricopeptide repeat proteins (CTPRs) make them very amenable to be rationally-designed into potential new PPI inhibitors. This study sets out to investigate whether this could be achieved through the grafting of binding peptides into the inter-repeat loops. The …
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Engineering Synthetic Biomolecular Condensates with Rationally-Designed Modular Repeat Proteins
… through phase separation of biomolecules such as proteins and nucleic acids, BCs exhibit remarkable structural and functional plasticity, resulting in their versatile material states and functional diversity across various cell types. Advances in microscopic techniques and computational …
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Development of peptide inhibitors as molecular therapeutics against tandem-repeat proteins
Tandem-repeat (TR) domains occur in approximately one third of all proteins, yet these domains are less well understood compared with their globular counterparts. TR proteins act as molecular scaffolds that facilitate protein-protein interactions (PPIs) in diverse biological contexts. In disease …
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Host-Dependent Expression, Transcriptional Regulation and Protection of Ehrlichia chaffeensis Tandem Repeat Proteins
… in mammalian hosts. The major immunoreactive proteins of E. chaffeensis include a group of tandem repeat proteins (TRPs) that are involved in various molecular strategies to reprogram the host cell during infection. The objectives of this dissertation were to examine host-specific expression …
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An interdisciplinary study of the mechanical and dynamic properties of α-solenoid repeat proteins
Tandem-repeat proteins differ from globular proteins, both in their biophysical characteristics and in how they interact with their respective partners, yet they comprise nearly one third of the human proteome and are central to many cellular processes and disease phenotypes. Repeat proteins have …
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Fine Mapping and Candidate Gene Discovery at the Rsv3 Locus
… four coiled-coil nucleotide-binding leucine-rich repeat proteins. In a related study a large population segregating at the Rsv3 locus was screened for resistance to facilitate future characterization of this region. The markers identified in this study will allow for more accurate marker-assisted …
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Design Strategies for Dynamic Self-assembled Protein Materials
… in soft-robotics and energy storage. Proteins are a useful class of molecules, because they contain a level of structural complexity beyond that of synthetic materials. They are an inherently 'green' material feedstock; made in a lab using microbes like E. coli. Additionally, with the …
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Ehrlichia chaffeensis Type 1 Secreted Effector TRP47 is a Novel SUMOylated Nucleomodulin
… of these effectors are the tandem repeat proteins (TRPs). In this study, E. chaffeensis TRP47 was identified as a target for host cell SUMOylation and as a novel nucleomodulin with DNA-binding activity and host nuclear localization. Using a microfluidic chip peptide array, a …
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Investigating the polyphosphate interactome with lysine- and histidine-rich proteins
… its simple structure. PolyP is comprised of repeated inorganic phosphate units forming high energy phosphoanhydride linked polymers. Numerous studies have highlighted its vast portfolio of functions that manifest their effects in a wide range of physiological pathways, including proteostasis, …
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Characterizing and engineering antibodies against the epidermal growth factor receptor
… different antibodies and three designed ankyrin repeat proteins binding to EGFR. In addition, we continued work on engineering novel antibodies against EGFR domains II and IV. Antibodies against these domains are hypothesized to directly inhibit receptor dimerization and subsequent activation, as …
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TRP75-Mediated STAT3 Activation Promotes Anti-Apoptotic Signaling and Ehrlichia chaffeensis Infection
… through the actions of secreted effector proteins, including the tandem repeat proteins (TRPs). The most well-characterized TRP, TRP120, uses multiple short linear motifs (SLiMs) to interact with host proteins and co-opt signaling axes to inhibit apoptosis. Interestingly, other ehrlichial …
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Engineering mono- and multi-valent inhibitors on a modular repeat-protein scaffold to target oncogenic Tankyrase
Tandem-repeat proteins are a class of proteins ubiquitous in nature and exploited in recent years in biotechnological and pharmaceutical applications due to their favourable biophysical properties. One such repeat motif, the tetratricopeptide repeat (TPR), has already been exploited for …
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CAG repeat expansions induce cytoplasmic RNA aggregation
Expansions of ‘CAG’ trinucleotide repeats in the genome can cause over a dozen diseases, including Huntington disease and several spinocerebellar ataxias. Short tracts of these ‘CAG’ repeats are benign; however, mutant alleles that harbor an abnormally large number of consecutive ‘CAG’ motifs can …
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Structural and biophysical characterization of membrane-coating proteins from the nuclear pore and the primary cilium
… trafficking by anchoring barrier forming FG-repeat proteins. At 60-120 MDa, the NPC is the largest macromolecular complex in the cell. However, it is a modular assembly formed by -30 different nucleoporins (Nups) arranged into stable subassemblies. One such module is the -600 kDa Y complex, …
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Characterisation of the PQ-loop repeat membrane protein family in arabidopsis thaliana
… describes the characterisation of the PQ-loop repeat family, a novel family of membrane proteins from Arabidopsis thaliana. The family consists of six members (AtPQL1-6) all predicted to contain seven transmembrane segments and two copies of the PQ-loop domain. The repeated PQ-loop domain is a …
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Recombinant Proteins for Biomedical Applications
… Here we focus on the use of recombinant proteins as molecular recognition motifs, wound healing biomaterials, and agents for cell cycle pathway elucidation are discussed. The author's primary project is described in chapters 2 and 3, and is focused on designed leucine-rich repeat proteins …
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The Discovery and Chemical Synthesis of Peptides and Proteins that Cross Biological Barriers
… task. Cell-penetrating peptides (CPPs) and proteins are used as carrier modules, tissue targeting agents, and probes to better understand disease states. In this work, we first investigate CPPs for peptide-mediated delivery of macromolecules. Despite the existence of thousands of CPP …
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Protein Engineering for Biomedicine and Beyond
… in which these scaffolds will be useful. Repeat proteins are instrumental in a wide variety of biological processes, including the recognition of pathogen-associated molecular patterns by the immune system. A number of successes using alternative immune system repeat protein scaffolds have …
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