Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 20 for “"repeat length"”.
-
Chromatin Structure of Schizosaccharomyces Pombe: From the Lowest to the Highest Orders of Folding
… methods for measuring the nucleosome repeat length, to determine the repeat length of Schizosaccharomyces pombe chromatin. I obtained a result of 156 $\pm$ 2 bp. Equivalent results are obtained using a psoralen crosslinking method for measuring the repeat length in viable spheroplasts. …
-
Associations Between Testosterone, Androgen Receptor Polymorphism, And Mood
… in the androgen receptor (polyglutamine [CAG] repeat length) may be important for this relationship. However, the relationship between testosterone, androgen receptor CAG repeat length, and depressive affect remains inconclusive. The current thesis examined the association between testosterone …
-
Investigations into the role of histone H2A ubiquitination in chromatin
… study, the relationship between the observed repeat length of chromatin and the rate of micrococcal nuclease digestion was investigated. The repeat length of the same starting chromatin preparation at equivalent extents of digestion was found to vary according to the rate of digestion.
-
Functional characterisation of the CAG polymorphism in the androgen receptor- in vitro and in vivo
… the AR coding region there is a polymorphic CAG repeat encoding a stretch of the amino acid glutamine (Q). The repeat length modulates receptor activity and is normally distributed between 10-30 CAG with a median length of 22 repeats in white men. At the start of this work, a negative linear …
-
The role of gene expression and aging in SCA1
… an autosomal dominant disorder caused by a CAG repeat expansion encoding a polyglutamine tract, where patients present with a lack of motor coordination including ataxia. The disease is characterized pathologically by loss of Purkinje cells (PCs) in the cerebellar cortex and neuronal loss in …
-
Deoxyribonuclease probing on sea urchin embryo chromatin
… low ionic strength buffers. The nucleosomal DNA repeat lengths for sea urchin blastula, gastrula and sperm have been determined using micrococcal nuclease. The repeat length for sperm is significantly larger than blastula and gastrula repeat lengths whereas blastula and gastrula repeat lengths …
-
Biochemical and structural studies of the FtsZ:FtsA complex and polymerising abilities of the FtsA protein
… that FtsA forms actin-like protofilaments with a repeat of 48 Å. An identical repeat length was observed when FtsA was polymerised using a lipid monolayer surface and FtsAs from three organisms formed polymers in cells when overexpressed, as observed by electron cryo-tomography. Mutants that …
-
Modeling Autism Spectrum Disorder: Fragile X syndrome and Rett syndrome
… potential. Fragile X syndrome is a trinucleotide repeat disorder where repeat expansion in the FMR1 5’ untranslated region triggers its methylation and represses its transcription. Yet we were unable to reproduce this methylation when we aggressively expanded the repeat length of mouse Fmr1, …
-
Das R6/2-Mausmodell der Chorea Huntington : verhaltensbiologische Charakteristika und der Einfluss ihres Diabetes mellitus
… disease (HD) gene with an increased CAG repeat length and develop a progressive neurological phenotype. Some of these mice were also found to develop a diabetes mellitus. The aim of this study was to search for behavioural tests that are best applicable to monitor the behavioural …
-
High force density linear permanent magnet motors : "electromagnetic muscle actuators"
… of large numbers of very small, actively-cooled repeat units, similar to the architecture of biological muscles, can provide greatly enhanced force density over extant designs. Our model is constructed by considering the motor winding as an active material, with its performance limited by the …
-
Testicular cancer; gonadal, sexual and psychological aspects of the disease and its treatment.
… correlated to the androgen receptor (AR) CAG repeat length, indicating a genetic variation in the recovery of sperm concentration. In article II, risk factors for developing hypogonadism were studied. SCT and RT treated were at higher risk of hypogonadism, 6 and 12 months post-treatment as …
-
Development of a multiscale coarse-grained chromatin model
… of chromatin fibers and the effect of nucleosome repeat length on chromatin compaction and inter-chromatin interactions. Together, our multiscale methodology provides a useful technique to extrapolate atomistic properties of nucleosomes to the modulation of large-scale chromatin organization.
-
Engineering Synthetic Biomolecular Condensates with Rationally-Designed Modular Repeat Proteins
… condensates based on consensus tetratricopeptide repeat (CTPR) proteins, modular folded non-globular domains, fused with low-complexity domains (LCDs), intrinsically disordered domains that drive phase separation. By systematically engineering a library of LCD-CTPR constructs sharing identical LCD …
-
Male Subfertility and Prostate Cancer Risk: Epidemiological and Genetic Studies
… androgen receptor (AR) gene harbors two codon repeat tracts: the CAG and GGN repeats, encoding corresponding amino acid sequences of variable length; the polyglutamine and polyglycine stretches, respectively. Variation in CAG repeat length had been associated with a number of andrological …
-
Deriving Structural Insights About Mesoscale Chromatin Folding Using Coarse-Grained Oligonucleosome Modeling
… the shape of the distribution of DNA fragment lengths resulting from ionizing-radiation damage. Pair-wise DNA-DNA contact data from DNA sequencing-based assays have the potential to provide locus-specific information about the relationships between chromatin structure and function. …
-
Beyond the basal ganglia
… weight loss increases with higher CAG repeat number in the mutant gene in both HD patients and R6/2 mice. This suggests that mutant huntingtin affects metabolic rate in a CAG repeat length dependant manner. The mechanism underlying this is unclear, but several regulators of metabolism, …
-
Molecular Genetic Analysis of Spinocerebellar Ataxia Type 7 and a Further Study on the RNA Interference Analysis
… by expansion of an unstable trinucleotide CAG repeat in ATXN7 gene which encodes Ataxin-7 protein. Normal ATXN7 alleles contain 4-35 CAG repeats. Expansion of CAG repeat in the mutant allele ranges from 36 to 460, with repeat length inversely correlated to the age of disease onset. However …
-
Sequence-structure correlations in the MaSp1 protein of N. clavipes dragline silk
… study explore the effects of the poly-alanine length of the N. clavipes MaSpi peptide sequence, solvent conditions, and nanomechanical loading conditions on secondary and tertiary structure predictions as well as the nanomechanical behavior of a unit cell of 15 strands with 900-1000 total …
-
Calcium Channel Dysfunction in Huntington’s Disease
… mutation in the huntingtin protein that lengthens a naturally occurring trinucleotide CAG repeat in exon 1 resulting in striatal degeneration. The mechanism by which striatal neurons undergo selective degeneration is not fully understood, although studies have suggested several different …
-
Magnetresonanztomographie-basierte Volumetrie bei hereditären spinozerebellären Ataxien
… von 82,3%. Die Repeatlängen des expandierten Allels zeigten eine negative Korrelation mit den Volumina von Mesenzephalon, Pons, Nucleus caudatus und Putamen bei SCA3, eine nur schwache Korrelation mit dem Pons bei SCA1 und indessen keine Korrelation bei SCA6. Der …