Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 30 for “"reoxygenation"”.
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role of pentazocine in an in vitro model of hypoxia/reoxygenation
… lipoil function in microglia following hypoxia/reoxygenation condition. BV2 cells were exposed to 3 hours of hypoxia and 24h of reoxygenation. Cells were treated with sigma-1 agonist (+)-Pentazocine, various bifunctional sigma-1 agonists conjugated with lipoil function and with lipoic acid …
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Reactive oxygen and nitrogen species production in cardiomyoblasts during hypoxia and reoxygenation
Hypoxia is a stress condition in which tissues are deprived of an adequate O2 supply; this may trigger cell death with pathological consequences in cardiovascular or neurodegenerative disease. Reperfusion is the restoration of an oxygenated blood supply to hypoxic tissue and can cause more cell …
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Reoxygenation of hypoxic coronary smooth muscle cells amplifies growth-retarding effects of ionizing irradiation
… undergoing alpha-irradiation are changed by reoxygenation. <br>Hypoxic and reoxygenated HCSMCs were irradiated with alpha-radiation at single doses of 4, 8 and 16 Gy using a 60Co-source. Vascular endothelial growth factor (VEGF) gene expression was dramatically suppressed in reoxygenated …
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Up-regulation of junctophilin-2 prevents ER stress and apoptosis in hypoxia/reoxygenation-stimulated H9c2 cells
Ischemic heart disease is the leading cause of death, and reperfusion which can restore blood flow is the primary therapy. However, reperfusion can induce further damage to cardiomyocytes, a condition described as ischemia-reperfusion (I/R) injury. I/R is now recognized as a combination determining …
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Extracellular adenosine as a novel neuroimmune modulator
… ambient air (normoxia). Mice required 6 hrs of reoxygenation before they could be successfully trained in novel object recognition post-hypoxia. Mice also lost the ability to be fear conditioned if trained post-hypoxia. When mice were administered interleukin-1 receptor antagonist prior to …
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Mitochondrial Dynamics: Exploring A Novel Target Against Myocardial Ischemia-Reperfusion Injury
… <p>Using an established model of hypoxia-reoxygenation (HR) in cultured HL-1 cardiomyocytes, we tested three hypotheses:</p> <p>I. subcellular redistribution of DRP1 is i) triggered by HR, and ii) plays a mechanistic role in HR-induced cytochrome c release and cell apoptosis; </p> <p>II. …
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The process of autophagy in an in vitro model of myocardial ischemia-reperfusion injury
… in ATP limits the autophagic response. In turn, reoxygenation of hypoxic cells exposed to 0.5% or 2.0% oxygen caused a partial restoration of ATP levels, which coincided with an activation of apoptosis. Importantly, the rates of these events were dependent on the degree of hypoxia. Interestingly, …
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The Oxygen Conforming Response in Active Mouse Skeletal Muscle In Vitro: A Pilot Study
… decrease force production, while subsequent reoxygenation produces a rapid recovery of force to pre-conformed levels. This readily reversible response remains poorly understood but appears to occur with minimal metabolic disturbance, suggesting a mechanism distinct from classical fatigue, …
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Hypoxic immunomodulation results in increased disease risk and altered behavior via non-canonical pathways
… psychological outcomes linked with hypoxia/reoxygenation injury. A causative role for brain-based IL-1 has been suggested in hypoxia-induced cognitive impairment and anxiety-like behaviors. However, the exact mechanism responsible has yet to be fully understood. Therefore, in this study we …
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PHOSPHODIESTERASE-5 INHIBITION: A NOVEL STRATEGY TO IMPROVE STEM CELL THERAPY IN THE HEART
… would improve their survival after ischemia/reoxygenation in vitro and enhance cardiac function following myocardial implantation in vivo. ASCs were treated with sildenafil or infected with PDE-5 silencing vector shRNA (shRNAPDE-5). The cells were subjected to simulated ischemia (SI) and …
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The central role of the neutrophil in ischaemic/reperfusion injury
… cell line (ECV304) after periods of hypoxia and reoxygenation. Neutrophil migration in response to factors secreted by the treated endothelial cells was then assessed. The genesis of an inappropriate oxidative burst by the neutrophil upon exposure to endothelial chemoattractants and adhesion …
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Understanding Radiation Resistance in Head and Neck Tumor Xenografts Using Diffuse Reflectance and Raman Spectroscopy
… for two weeks. We observed greater rate of reoxygenation in radiation-resistant tumors which was accompanied with greater content of hypoxia inducible factor-1α (HIF-1α). Our results indicate that reduced oxygen consumption rate can potentially play a significant role in promoting radiation …
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Cardiac mitochondrial respiration in two rodent models of obesity
… isolated mitochondria were stressed by anoxia-reoxygenation. We speculate that this may be dure to reduced expression of mitochondrial respiratory chain complexes in the insulin resistant rat heart. For the mouse model and type II diabetes we found increased respiratory capacity at 10-12 weeks, …
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Ischemic Preconditioning Protects Adult Rat Cardiomyocytes Against Necrosis but not Apoptosis, via Activation of PKG
… myocytes in the ischemic buffer for 90 min and reoxygenation (RO) for 120 min in the normal medium. Necrosis was determined by trypan blue exclusion and apoptosis was assessed by TUNEL assay. IPC reduced necrosis as shown by significant decrease in trypan blue positive cells as compared to …
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Cardioprotective mechanisms targeting thiol redox homeostasis and mitochondrial bioenergetics
… and redox signaling. In cell models of hypoxia/reoxygenation, targeting glutathione reductase expression influences the cells sensitivity to mitochondrial dysfunction. Several hallmark features of the cardioprotective phenotype include reductions in myocardial infarction, resistance to …
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Mapping viability and oxygen concentration in spheroids: A noninvasive approach with EPR imaging
… context for investigating the phenomenon of reoxygenation and the interplay of local (O$\sb2$) and cellular viability in the response of tumors to various therapeutic regimens. There are, however, certain limitations of the methodology currently used to measure local (O$\sb2$) and viability …
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Molecular Mechanisms Underlying Aerobic Respiration and Heme Regulation in Yeast
… as one of the fast responders to hypoxia and reoxygenation. Here we have studied the regulation of the transcriptional activity of Gis1 by heme. Gis1 belongs to the JmjC family of histone demethylases. Gis1 protein has two heme regulatory motifs (HRM). We show that the DNA binding zinc finger …
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NOVEL STRATEGIES TO IMPROVE METABOLIC PARAMETERS AND PRECONDITION DIABETIC HEARTS AGAINST ISCHEMIA/REPERFUSION INJURY
… and were subjected to simulated ischemia and reoxygenation (SI/RO) for cell viability and reactive oxygen species (ROS) measurement. Another set were subjected to IRI in a Langendorff model. Plasma samples were taken to measure plasma concentrations of cytokines. For miR expression, total RNA …
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The Role of Cytochrome P450 2E1 in Hepatic Ischemia Reperfusion Injury and Reactive Oxygen Species Formation
… clones were subjected to hypoxia (10 h) and reoxygenation (0.5 h), and cell death and ROS generation were quantitated using LDH and flow cytometry, respectively. Compared with Control clone, the selected CYP2E1 clone showed a 720-fold increase in CYP2E1 expression and a prominent band in the …
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Expression and secretion of basigin by human placental trophoblast cells occurs via microvesicle shedding and is a regulated process
… cells is altered in response to hypoxia/reoxygenation injury; 6) the inflammatory cytokine interleukin-1β (IL-1β) and transforming growth factor-β1 (TGF-β1) regulate BSG protein abundance in microvesicles at the post-transcriptional level; 7) recombinant BSG protein (rBSG) stimulates …
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