Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"relative quantification"”.
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ROLE OF CKD AND CASPASE-1 IN NEOINTIMAL HYPERPLASIA DEVELOPMENT
… casp-1 inhibition. In an ex-vivo model using relative quantification we found that VSMC contractile markers α -Actin, Calponin, SM-22, and Smoothelin gene expression of CKD mouse carotid VSMC were higher in casp-1 knockout mice when compared to wild-type (1.40, 1.28, 1.22, 1.41 respectively). …
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Effects of Heterodera Glycines Population Densities on Fusarium Solani F. Sp. Glycines Colonization of Soybean Roots and Development of Sudden Death Syndrome
… Therefore, an alternative method was explored, relative quantification with real-time quantitative PCR (QPCR), to estimate fungal colonization of soybean roots. QPCR was more sensitive than the plating bioassays at detecting the fungus: Fsg DNA was detected in 371 out of 372 root samples with …
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DECIPHERING THE ORAL MICROBIOTA IN FOOD ALLERGY: INSIGHTS INTO THE CANDIDATE PHYLA RADIATION AND NOVEL METHODOLOGICAL APPROACHES
… of allergic children using absolute rather than relative quantification, revealing patterns of microbial susceptibility and resilience to immune pressure. We also studied children who achieved antigen tolerance, observing microbial shifts that partially restored a healthy-like composition. …
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Differences in miR-122 and miR-191 Expression in HBV- versus HCV-associated Hepatocellular Carcinoma
… non-tumor tissue, and normal liver tissue. Relative quantification of miR-122 and miR-191 in 16 HCV-associated HCC, 10 HBV-associated HCC, the respective neighboring non-tumor tissue, and 9 normal liver tissue samples was performed using RT-PCR. This thesis shows that miR-122 expression …
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Genetic association between schizophrenia and type-2 diabetes
… levels of the genes of interest. A relative quantification method (the<br/>comparative Ct method; 2-ΔΔCt) was used to analyze the differences in gene<br/>expression between clozapine-treated and untreated cells. Two house-keeping<br/>genes (HKGs), beta-actin (ACTB) and glyceraldehyde …
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Identification, Sequencing, Expression and Evolutionary Relationships of the 11S Seed Storage Protein Gene in Chenopodium quinoa Willd.
… assortment of essential amino acids with relatively high levels of glutamate/glutamine, aspartate/asparagine, arginine, serine, leucine and glycine, typical of other 11S seed storage proteins. Total RNA and globulin was extracted from seed collected at different developmental intervals …
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Benchmarking methods for label-free quantitative proteomics data analysis
… is the predominant technology for protein quantification. In particular, MS-based label-free quantification (LFQ) proteomics can quantify thousands of proteins in complex biological samples. However, the molecular heterogeneity of such samples and the technological limitations of MS-based …
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Proteomic Analysis of Three Dimensional Organotypic Liver Models
… mass spectrometry (LC-MS/MS) for relative quantification of protein abundances by measuring intensities of their corresponding peptides. Organotypic 3D liver models have been developed in our laboratory that consist of hepatocytes and liver sinusoidal endothelial cells (LSECs) …
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Characterisation of protein phosphorylation in Arabidopsis thaliana and Chlamydomonas reinhardtii based on affinity chromatography and mass spectrometry
… a protein can be influenced by phosphorylation, quantification of phosphorylation delivers more detailed information on protein regulation by phosphorylation. Using an approach involving ICP-MS it was possible to monitor the overall protein phosphorylation degree for different developmental …
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The effects of maternal diets, varying in fat content, on proximal hepatic and skeletal muscle insulin signalling in neonatal wistar rat offspring
… and skeletal muscle was performed followed by relative quantification with image analysis software. Further, Aspalathus linearis (Al) extract was orally administered to mothers during gestation in the 10% (Control-Al) and 40% (HFD-Al) diets at a dose of 150 mg/kg. Body weight, food intake and …
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Investigation of sodium binding through implementation and application of single- and double-quantum filtered 23Na NMR spectroscopy
… to investigate sodium ion binding, using a relative ratio of bound to total sodium, in specific food systems, and how that binding changes as a function of sodium ion addition and removal from the matrix. However, the SQ and DQF 23Na NMR methods have never before been established here at the …
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A proteomic approach to investigate the response of tef (Eragrostis tef) to drought
… and physiologically characterised using relative water content (RWC), electrolyte leakage and chlorophyll fluorescence measurements, to establish critical water content stages for investigation of changes to the tef proteome in response to dehydration stress. Physiological testing showed …
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Investigating the relationship between miRNA expression and epithelial mesenchymal transition in colorectal cancer
… expression levels were ascertained using the relative quantification method. Moreover, the clinical significance of the two miRNAs was evaluated in relation to MSI status. Therefore, IHC analysis of MLH1, MSH2 and MSH6 mismatch repair proteins was performed on the Ventana platform. Statistical …
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Immune gene expression and diversity in relation to gastrointestinal parasite burden in small mammals
… conditions. I established the method of relative quantification of mRNA on liver and spleen samples of both species in our laboratory. As there was no available information on nucleic sequences of potential reference genes in both species, PCR primer systems that were established in …
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IDENTIFICATION OF MECHANISMS OF DELAYED PUBERTY ON BONE STRENGTH DEFICITS DURING DEVELOPMENT
… group and 63.9% lower in the ER group. The relative quantification (RQ) of RUNX2 gene expression was lowest in control followed by GnRH-a and highest in ER group although no statistical significance was observed between any groups. Thus our data infers that 30% energy restriction does not …
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Developmental Gene Expression in the Small Intestine of Chickens from Lines Divergently Selected for High or Low Juvenile Body Weight
… was assayed by real-time PCR using the relative quantification method. In comparing HH and LL males and females there was a line by segment interaction in PepT1 gene expression, with no segment difference in HH and greatest expression in the ileum of the LL (P < 0.05). There was also a …
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Heat stress: A risk factor for skin carcinogenesis
… rate and incidence of cutaneous tumour formation relative to UVB alone. However, the effects of UVB plus heat on human epidermal cells have yet to be determined. Furthermore, the exact mechanisms responsible for the observed effects of heat stress need to be characterised in skin keratinocytes to …
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Biochemistry in healthy and neoplastic human tissues: metabolic alteration revealed by HR-MAS nuclear magnetic resonance spectroscopy
… human meningiomas and for the validation of the quantification procedure of in vivo MR spectra. By using one- and two dimensional experiments, several metabolites in different histological subtypes of meningiomas, were identified. The spectroscopic data confirmed the presence of the typical …
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Development and Validation of Quantitative PCR Assays for DNA-Based Newborn Screening of 22q11.2 Deletion Syndrome, Spinal Muscular Atrophy, Severe Combined Immunodeficiency and Congenital Cytomegalovirus Infection
… detection in the 22q11.2 critical region, and quantification of the SCID biomarker, T-cell receptor excision circles (TRECs) and CMV were all required for disease confirmation. SMA and 22q11.2 gene deletions were accurately detected using the OpenArray system, a first for the technology. The …