Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 61 for “"relative expression"”.
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Investigation of relative expression level of SLC4 bicarbonate transporter family in mouse and Human corneal endothelial cells
… corneal diseases in human. Aim: To characterize relative expression levels of SLC4 members in mouse and human corneal endothelium, using qRT-PCR, to identify further important members besides SLC4A11 and SLC4A4, and to explore how close to the base line levels the gene expression levels remain …
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Relative Expression of Germination Genes SleB, GerAC, and Gpr in Heat Treated Bacillus licheniformis and Bacillus subtilis
… and <em>SleB</em> genes to evaluate their relative expression and potential for indicators of lag phase in germination. Gene expression studies with qPCR were carried out during germination in <em>Bacillus subtilis </em>and <em>Bacillus licheniformis</em> spores that were given a heat shock …
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<i>PTHR1/SOX9</i> and <i>IDH1/IDH2</i> Relative Expression in Primary Chondrocyte and Chondrosarcoma Cells Under the Synergistic Influence of Inducible Hypoxia and Extracellular Acidosis
… is to examine how these stressors affect gene expression in primary chondrocytes and chondrosarcomas. In this study, one primary chondrocyte cell line (CON5) and two chondrosarcoma grade II cell lines, JJ012-<em>IDH1</em> mutant and SW1353-<em> IDH2</em> mutant, were grown in four experimental …
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Evaluation of the Hypothalamic Kisspeptin System Throughout the Estrous Cycle and During the Attainment of Puberty in Gilts
… cycle when MBH, AP, and blood were collected. Relative expression of hypothalamic Kiss1, Kiss1R, ER-alpha, ER-beta and beta-actin was determined using real-time reverse transcriptase PCR. Fold changes in relative expression were determined using the Relative Expression Software Tool. Relative …
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Cucurbit Downy Mildew (Pseudoperonospora cubensis): Cucumber Resistance
… a molecular study was conducted, comparing the relative expression of genes encoding a basic PR-1 protein, a cytosolic ascorbate peroxidase protein and three resistance (R) gene proteins, in nineteen cultivars. All of the selected genes were analyzed using real-time PCR. The relative expression …
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Rational Engineering of Expression Level of Multi-Gene Systems encoding Natural Product Biosynthesis in Streptomyces
… novel compounds. However, cloning and controlled expression of these large BGCs in a heterologous host require tedious optimization on a case-by-case basis. This dissertation presents a synthetic biology platform to rapidly reconstitute BGCs by refactoring and physically piecing DNA fragments …
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Characterization of Host Immune Responses to Eimeria adenoeides Infection in Turkey Poults
… area occupied by CD4+ and CD8+ lymphocytes, and relative expression of cytokines (CXCLi2, IL1β, IFNγ, IL10, and IL13) was observed in the ceca following infection. In experiment 3 (Chapter IV), we attempted to model the field situation by investigating the acquisition of immunity under conditions …
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Expression Of Nicotinic Acetylcholine Receptor mRNA As a Function Of Age In Whole Hippocampus Preparations From Wistar Rats
… ages (10-90 days old) were analyzed for the mRNA expression of the alpha 2, alpha 3, alpha 4, alpha 5, alpha 7, beta 2, beta 3, and beta 4 neuronal nicotinic acetylcholine receptor subunits. To do so, RNA was isolated from acutely isolated hippocampal samples, converted to cDNA by means of a …
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HYPOXIA-REGULATED EXPRESSION OF GLUT-1 IN GBM CELL LINES
… in low oxygen (hypoxic) environments. Changes in relative oxygen concentration can alter gene expression in tumors to allow for their selective growth. The result of such changes allows the tumor to adapt its cellular metabolism and promote tumor progression. Most notably, hypoxic conditions …
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Retinal development and age related degeneration following gestational lead exposure
… assays. RESULTS: GLE decreased the relative expression of rod specific genes at PN2; delayed the differentiation and functional development of rod photoreceptors and bipolar cells by 2-3 days; increased and prolonged the glutamatergic response of post-mitotic rod and bipolar …
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Pathophysiological changes in cardiomyocyte metabolism and bioenergetics during stress-induced cardiomyopathy.
… chain reaction (RT-PCR) and Western blot for relative expression of lipid transporters were used to study cardiomyocyte metabolism. Confocal microscopy was used to study Ca2+ handling. The presented thesis demonstrates that acute exposure to an extreme dose of isoprenaline decreased basic …
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Role of Wnt signaling in proliferation of human bone marrow-derived mesenchymal stem cells
… molecules on the proliferation of hMSCs. The relative expression of secreted frizzled-related protein 1, an inhibitor for canonical Wnt signaling, was declined with passage, but nuclear β-catenin level was not related to sFRP1. Interestingly, specific knockdown of sFRP1 by shRNA in hMSCs …
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Next-generation sequencing analysis and RNA editing in human brain and glioma
… in use today for the analysis of gene expression and transcriptome variation in biological samples (Zhong Wang et al. 2009). As of 2011, the NCBI Sequence Read Archive (SRA) surpassed 100 terabases of sequence data, comprising nearly 40,000 RNA and 260,000 DNA sequencing projects. In …
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The spatial and temporal regulation of the transcriptome during plant development
… We detected specific light induced gene expression for the root phototropism gene RPT2 in the apical hook, and also phenotypes in Arabidopsis rpt2 mutants demonstrating that the gene is necessary for normal photomorphogenesis in the seedling apex. Significantly, expression of the …
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Lung disease diagnosis from gene expression profiles
… a broad range of lung diseases. We used gene expression microarray data from five different lung diseases—lung adenocarcinoma, lung squamous cell carcinoma, large cell lung carcinoma, chronic obstructive pulmonary disease, and asthma—as well as a non-diseased phenotype, to train a …
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Identifying tightly regulated and variably expressed networks by differential rank conservation (DIRAC)
… disease process. This approach is based on the relative expression values of participating genes—i.e., the ordering of expression within pathway profiles. DIRAC provides quantitative measures of how network rankings differ either among networks for a selected phenotype or among phenotypes for a …
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Regulation of LKB1-STRAD-MO25 Complex Expression and Activation of AMPK in Skeletal Muscle by Thyroid Hormone
… treatment and prevention of type 2 diabetes. The expression of all of the isoforms of the subunits of AMPK and AMPK activity are increased in skeletal muscle tissue of hyperthyroid rats. Activity of AMPK is regulated by an upstream kinase (AMPKK). The LKB1-STRAD-MO25 complex is a major AMPKK in …
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A Larval Zebrafish (Danio Rerio) Model of Adherent-Invasive Escherichia Coli Infections
… neutrophil recruitment to the gut and increased relative expression of the genes that encode proinflammatory cytokines. In addition, we showed that two AIEC virulence factors, FimH and IbeA, play a role in AIEC colonization and contribute to intestinal inflammation in larval zebrafish, similarly …
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Anti-Cancer Effects Of Garcinol In Pancreatic Cancer Transgenic Mouse Model
… suppressor miRNA with Garcinol treatment. Lower expression levels of miRNA 23a and higher expression levels miRNA 451a after Garcinol treatment were validated by RTPCR. Relative mRNA expressions levels of MMP9, CCND1 and Notch1 were found to be decreased in KGr and KGG groups compared to KC group …
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Characterizing Populations of Non-Coding RNAs in <i>Karenia brevis</i> at Different Times of the Diel Cycle
… other organisms play a role in regulating gene expression. To test this prediction, I extracted total RNA from cells grown under different culture conditions, isolated and cloned the dsRNAs and miRNAs separately, and sequenced all transcripts from each sample. Bioinformatic analyses were used to …
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