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Showing 1 to 20 of 103 for “"regulon"”.
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Delineating the Upc2A Regulon in Candida glabrata
… in C. glabrata. We aimed to delineate the Upc2A regulon to determine its target genes involved in FLU resistance. Transcriptome sequencing (RNA-seq) analysis was used to compare gene expression profiles of: a) wild-type (WT) strains with and without UPC2A under non-stressed conditions; b) those …
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Functional Characterization of the Streptococcus sanguinis com Regulon
… species have suggested functions for the com regulon apart from DNA uptake. Here we characterized functions of the S. sanguinis com regulon genes in genetic competence, IE virulence, and biofilm formation. Our findings indicated that the early regulatory genes and those under the control of …
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Analysis of the hex regulon in Pseudomonas aeruginosa
… The activity of the enzymes in the hex regulon are required for catabolism of glucose and these activities are coordinately regulated. Previous work has shown that these enzymes are induced when grown on carbohydrates and catabolite repressed when grown on TCA cycle intermediates. The …
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Global characterization of the Pho regulon in Caulobacter crescentus
… regulator PhoB to globally map the Pho regulon in Caulobacter in both phosphate-starved and -replete conditions. I find that the regulatory regions of over 50 genes are bound by PhoB following phosphate limitation, and I identify a consensus PhoB binding motif in Caulobacter. I then …
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Studying the Competence Regulon Quorum Sensing Circuity in Streptococcus mitis
… bacterium, utilizes the comABCDE competence regulon QS circuity. However, the competence stimulating peptide (CSP), the signaling molecule, that is responsible for QS activation, is not identified yet. In this work, we set out to identify the CSP of S. mitis and study the molecular mechanisms …
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Transcriptional Regulation of the MGA Virulence Regulon in Streptococcus Pyogenes
The group A streptococcus (GAS) is a strict human pathogen that is capable of causing an array of diseases ranging from pharyngitis (strep throat) to necrotizing faciitis (flesh-eating disease). The GAS possesses a transcriptional regulator called Mga, or the multiple gene regulator of the GAS, …
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Characterization of the A/B regulon in tobacco (Nicotiana tabacum)
… Results of this study suggest that the A/B regulon is not limited to alkaloid biosynthetic genes, but includes multiple genes with defense-related functions. QRT-PCR analysis of nicotine biosynthetic genes and genes represented by confirmed differentially expressed FDD clones showed …
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Investigation of the quorum-sensing regulon in the corn pathogen Pantoea stewartii
… method identified even more genes in the QS regulon that the proteomic approach overlooked. RNA-Seq analysis of rRNA-depleted RNA from two strains of P. stewartii was used as a screen to help identify 11 promoters, subsequently shown to be directly regulated by EsaR in vitro. Most of the …
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Contribución del regulon Rcs a la virulencia de Salmonella análisis genético y molecular
En el momento de iniciar esta tesis, trabajos recientes de nuestro laboratorio en colaboración con el Dr. García del Portillo habían descrito que alelos viables del gen esencial igaA activaban el sistema de transducción de señales Rcs, y que dicha activación atenuaba la virulencia de Salmonella. Se …
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Total Genomic Transcriptional Analysis of the Fatty Acid Degradation Regulon of Escherichia Coli
The second ORF, b2323 is the fabB gene which produces beta-ketoacyl-ACP synthase I. This is one of two long chain specific condensing enzymes in E. coli and is required for unsaturated fatty acid biosynthesis. The other enzyme necessary for producing unsaturated fatty acids is …
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Studies on the operator-repressor-effector interactions in the glp regulon of Escherichia Coli K-12
All of the glp genes of Escherichia coli are subject to negative regulation by the glpR-encoded repressor (GlpR). Comparison of the repressor binding affinity for consensus and altered consensus operator regions showed that positions 3, 4, 5, 7 and 8 bp removed from the center of operator symmetry …
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Unraveling the ORE1 regulon in Arabidopsis thaliana : molecular and functional characterization of up- and down-stream components
Leaf senescence is an active process required for plant survival, and it is flexibly controlled, allowing plant adaptation to environmental conditions. Although senescence is largely an age-dependent process, it can be triggered by environmental signals and stresses. Leaf senescence coordinates the …
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The role of coordinated regulation and aromatic metabolites in activating the mar/sox/rob regulon of Escherichia coli
… master regulators of the extensive mar/sox/rob regulon that has been directly implicated in multidrug resistance found in clinical and laboratory isolates. In this work, we examine the degree of genetic cross-talk between these regulatory systems and the cooperative role of these three …
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Structure and function of the repressor and operators of the sn-glycerol-3-phosphate regulon of Escherichia coli K-12
… negatively regulates the expression of the g/p regulon, map near minute 75 on the linkage map of Escherichia coli K-12. In the present study, the nucleotide sequence of the 2895 base pair of DNA containing the glpD control region and the glpE, glpG, glpR genes was determined. The translation …
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Purification and Characterization of glpX-Encoded Fructose 1,6-Bisphosphatase, a New Enzyme of the Glycerol 3-Phosphate Regulon of Escherichia coli
… is mediated by gene products of the glp regulon. The regulon encompasses five operons, including the glpFKX operon. Although glpF and glpK encode glycerol diffusion facilitator and glycerol kinase,respectively, the function of glpX was unknown. In the present work, we show that glpX …
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Genetic analysis of conserved residues in PhoU of Escherichia coli
The Pho regulon is controlled by the PstSCAB transporter, PhoU, and the two-component proteins, PhoB and PhoR. PhoU is a negative regulator of the Pho regulon under phosphate-replete conditions. How PhoU functions is unknown. Many PhoU homologues are found widely throughout prokaryotic domains. …
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Existing regulatory circuitries govern backbone and acquired host association factors in the human pathogen Vibrio parahaemolyticus
… analysis of a conserved host association regulon (GacA) in the closely related V. fischeri (symbiont) and V. parahaemolyticus (pathogen), to exploit key lifestyle differences will expose genes that are unique to pathogenesis. In this study we identified a small, core regulon that is GacA …
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Systematic determination of a transcription factor/binding site functional interaction landscape
… interact with a given regulator? How does the regulon sample from available functional sequences? How independent is each half of a two part binding site? How do mutations in the regulator impact the regulon? Using PhoP, the regulator from the E. coli magnesium-responsive two-component system …
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Responses to Reactive Oxygen Species in Escherichia Coli: The Roles of Aconitase a and Fur Protein
The Fur regulon is a well-characterized regulon that monitors intracellular iron concentrations by monitoring concentrations of the ferrous ion. We observed that sub-micromolar concentrations of H2O2 in vivo can cause E. coli catalase and NADH peroxidase mutants (Hpx -) to lose control of their …
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Characterization of AgaR and YihW, Members of the DeoR Family of Transcriptional Regulators, and GlpE, a Rhodanese Belonging to the GlpR Regulon, Also a Member of the DeoR Family
AgaR, a protein in <i>Escherichia coli</i> thought to control the metabolism of N-acetylgalactosamine, is a member of the DeoR family of transcriptional regulators. Three transcriptional promoters within a cluster of genes containing the gene for AgaR were identified, specific for <i>agaR, agaZ</i> …
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