Global ETD Search
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Showing 1 to 12 of 12 for “"regulatory light chain"”.
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Orientation of the Cardiac Myosin Regulatory Light Chain Determined by Polarized Fluorescence
The regulatory light chain (RLC) of myosin is a component of the lever arm of the<br/>myosin motor in muscle thick filaments. Phosphorylation of RLC by myosin light<br/>chain kinase (MLCK) modulates the force and speed of muscle contraction, but the mechanisms of RLC‐mediated regulation in striated …
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The influence of myosin regulatory light chain phosphorylation on the contractile performance of fatigued mammalian skeletal muscle
ABSTRACT The myosm regulatory light chain (RLC) of type II fibres is phosphorylated by Ca2+ -calmodulin dependent myosin light chain kinase (skMLCK) during muscular activation. The purpose of this study was to explore the effect of skMLCK gene ablation on the fatigability of mouse skeletal muscles …
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Conformational Studies of Myosin and Actin with Calibrated Resonance Energy Transfer
… myosin's nucleotide site, subfragment-2, and regulatory light chain to examine nucleotide analog-induced structural transitions. The distances between the three positions were measured in the presence of different nucleotide analogs. No distance change was considered to be statistically …
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Proteomic analysis of the olfactory mucosa, thoracic aorta, urinary bladder, and descending colon of smooth muscle alpha actin null mutant mice
… levels of skeletal muscle myosin essential and regulatory light chain, along with gamma actin, were increased, whereas levels of alpha cardiac muscle actin were decreased. In the urinary bladder, a loss of SMAA expression was accompanied by a decrease in skeletal muscle myosin essential and …
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Chemical Biology Tools for Biochemical and Functional Analysis of Myosin Phosphatase
… in AF to dysregulation of myosin light chain phosphatase (MLCP), a holoenzyme composed of a catalytic subunit (PP1cβ) and a regulatory subunit that targets the phosphatase to sarcomeric substrates. PPP1R12C is an atrial-enriched MLCP regulatory subunit that directs PP1cβ to the …
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Mechanisms of myosin regulation and function during tissue folding
… and dephosphorylation of the myosin regulatory light chain are required for myosin pulsing and step-wise apical constriction. Uncoupling myosin from its upstream regulators resulted in loss of pulsatile myosin behavior and continuous, instead of incremental, apical constriction. A …
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Force potentiation as a modulator of contractile performance: Implications for control of skeletal muscle force and energetics of work
… muscles from wildtype and skeletal myosin light chain kinase knockout (skMLCK-/-) mice that does not express the primary mechanism for PTP: myosin regulatory light chain (RLC) phosphorylation. In contrast to smooth/cardiac muscles where RLC phosphorylation is indispensable, its precise …
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Development and application of chemical tools for investigating dynamic processes in cell migration
… by phosphorylation of the associated myosin regulatory light chain (mRLC) protein at Serl9. To generate a photoactivated mRLC variant that offers control over the timing and localization of myosin activity, the mRLC has been prepared by expressed protein ligation for the site-specific …
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Interactions of Smooth Muscle Myosin Filaments with Actin and Myosin Light Chain Kinase: Insights into the Mechanics of Smooth Muscle Contraction
… by phosphorylation of the myosin regulatory light chain (RLC). Upon phosphorylation of Serine 19 of the RLC of SMM by myosin light chain kinase (MLCK), SMM filaments and actin (thin) filaments slide over one another and the cell contracts. The effect of the filamentous conformation …
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Genetic Approaches to Study Tissue Morphogenesis in Drosophila
… is regulated by phosphorylation of the myosin regulatory light chain (MRCL, encoded by spaghetti squash or sqh in Drosophila) first on Ser-21 and subsequently on Thr-20. These phosphorylation events are positively controlled by a variety of kinases including myosin light chain kinase, Rho …
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Mechanisms affecting post-activation potentiation following voluntary isokinetic knee extension
… function, possibly including changes in myosin regulatory light chain (RLC) phosphorylation and increased intracellular Ca2+ release and sensitivity may have contributed to the observed PAP. It is clear from the literature and the results of Study 1 that there is a significant inter-individual …
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Proteomic investigation of the molecular targets of mycophenolic acid in human cells
… thioredoxin domain-containing protein 12, myosin regulatory light chain 2, and profilin 1), while 5 proteins were down-regulated (protein SET, stathmin, 40S ribosomal protein S12, histone H2B type 1 A, and histone H2B type 1-C/E/F/G/I). Functional annotation tool analysis showed that MPA modulated …