Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 295 for “"regulatory elements"”.
-
RNA regulatory elements and translational control
… RNA-Seq data were used to examine genetic elements that control translation, and were found to differ between *Salmonella* and *Listeria*. In *Listeria*, we have found that a subset of efficiently translated genes had ribosomal footprints seven nucleotides upstream of the initiation …
-
The specificity and evolution of gene regulatory elements
… transcription factors, microRNAs (miRNAs), cis-regulatory DNA and RNA. It is the quantitative and combinatorial interactions of these regulatory elements that defines gene expression, but these interactions are incompletely understood. In this thesis, I present two new methods for determining …
-
Identifying Splicing Regulatory Elements with de Bruijn Graphs
Splicing regulatory elements (SREs) are short, degenerate sequences on pre-mRNA molecules that enhance or inhibit the splicing process via the binding of splicing factors, proteins that regulate the functioning of the spliceosome. Existing methods for identifying SREs in a genome are either …
-
Investigation of transcription factor binding at distal regulatory elements
… is in part orchestrated by a class of remote regulatory elements, termed enhancers, which are brought into contact with promoters via DNA looping. Enhancers typically contain clusters of transcription factor binding sites, and TF recruitment to them is thought to play a key role in …
-
Identification and functional characterization of microRNA regulatory elements in Brassicaceae
… just initiated. With the aim to understand the regulatory mechanism of plant microRNA in evolutionary tem, and identify the most relevant cisregulatory elements in some microRNAs for improving the agriculture in the future, this study was carried out. microRNA390 is one of the many conserved …
-
Genome-Wide Human-Specific RNA Regulatory Elements in the Brain
… multiple transcripts with distinct coding and regulatory sequences giving rise to multifaceted protein function. RNA binding proteins dictate alternative RNA processing which is a key player in most, if not all, biological processes. Understanding how RNA binding proteins regulate RNA steady …
-
Computational discovery of Cis-regulatory elements in multiple drosophila species
… factors control gene expression by binding regulatory DNA segments called cis-regulatory modules (CRMs). The increasing number of sequenced genomes of multicellular eukaryotes along with high-throughput methods such as whole genome microarray expression data allows for systematic …
-
Identification and characterization of conserved regulatory elements by comparative genomics
… is to use fugu to identify conserved noncoding elements (CNEs) in genes expressing in the human forebrain, and to characterize selected CNEs in transgenic mice. To this end, genomic sequences for 50 human genes were aligned with their orthologous sequences in mouse and fugu. 206 CNEs associated …
-
Computational analysis of upstream regulatory elements regulating plant chlorophyll degradation
… are tightly regulated. Disruption of the regulatory pathway for chlorophyll breakdown by exposure to cold temperature episodes in canola seed is the basis of the economically significant ""green seed problem"". A major controlling step in the chlorophyll degradation pathway involves the …
-
Discovering Conserved cis-Regulatory Elements That Regulate Expression in Caenorhabditis elegans
… 1) To catalog all <italic>cis</italic>-regulatory elements within the intergenic and intronic regions surrounding every gene in <italic>C.elegans</italic>: i.e. the regulome) and: 2) to determine which cis-regulatory elements are associated with expression under specific conditions. We …
-
A knowledgebase of stress reponsive gene regulatory elements in arabidopsis Thaliana
… factor binding sites. Here transcriptional elements play a key role in mediating responses to environmental stress where each transcription factor binding site may constitute a potential response to a stress signal. Arabidopsis thaliana, a model organism, can be used to identify the …
-
Characterization of Cis-Regulatory Elements Controlling Repo Transcription in Drosophila Melanogaster
… In 2005, Lee and Jones defined a 4.3 kb cis-regulatory DNA region that recapitulates the endogenous Repo expression pattern dependent on a single Gcm binding site. Within that region, are three different cis-regulatory elements that drive cell-specific expression independent of Gcm binding …
-
A high-resolution study of the chromatin environment around regulatory elements
… but also distribution of modifications within regulatory regions. One approach aimed at improving our understanding is to cluster regulatory regions based on information contained in signals that describe, at a high-resolution, the distribution of these modifications. In this thesis we develop …
-
Characterization of Loop Extrusion and Regulatory Elements in IgH Locus Structure and Function
… Igh locus. Furthermore, we demonstrate that cis regulatory elements regulate accessibility in the Igh locus, which is correlated with rates of V-DJH joining. Collectively, this demonstrates the importance of cis regulatory elements in Igh locus organization and function.
-
Tumor Response TCF-4/β-Catenin Regulatory Elements for Enhancing Cancer Gene Therapies
… normal and tumor cell lines. The Tcf-4/β-catenin elements conferred tumor specific expression of HSV thymidine kinase (HSV-TK) gene, resulting in selective metabolism of ganciclovir and cell killing of only tumor cell lines. There was no detectable expression of HSV thymidine kinase expression in …
-
THE APPLICATION OF HISTONE ANALYSIS FOR IDENTIFYING DISTAL REGULATORY ELEMENTS - REGULATION OF FSHR
… used technically to identify numerous distal regulatory elements and furthered our knowledge of transcriptional regulation and cell-specific gene regulation. Fshr is a gene expressed only in testicular Sertoli cells and ovarian granulosa cells and its expression is critical for proper gonad …
-
Developing and applying transient expression systems to identify and understand gene regulatory elements in plants
… to identify, characterize and design plant gene regulatory elements. To allow for the scale of MPRAs, I develop a high-efficiency transformation protocol for maize mesophyll protoplasts. Using this protocol, I can transform millions of maize mesophyll protoplasts without losing viability. I use …
-
Identification and Characterization of Transcriptional Regulatory Elements for Liver-Specific Expression of Rabbit CYP2C Genes
… demonstrated the presence of multiple cis-acting regulatory elements in three closely related CYP2C genes (2C1, 2C2 and 2C3). The most active promoter motifs which confer the hepatic specific activation of these genes are located about 100 bp upstream of their mRNA initiation sites. Both DNA …
-
DISCOVERY OF NOVEL EPIGENETIC MECHANISMS OF CARCINOGENESIS BY GENOME-WIDE PROFILING OF NON-CODING REGULATORY ELEMENTS
… informative of the activity states of distal regulatory elements. Therefore we can use these epigenetic marks to study important regions of the cancer genome that are key regulators of transcription with unprecedented resolution and accuracy. In this work, in-depth studies of epigenetic …
Page 1 of 15