Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 28 for “"regulation of mRNA"”.
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Understanding regulation of mRNA by RNA binding proteins
Posttranscriptional regulation of mRNA by RNA-binding proteins plays key roles in regulating the transcriptome over the course of development, between tissues and in disease states. The specific interactions between mRNA and protein are controlled by the proteins' inherent affinities for different …
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Regulation of mRNA decay by Pumilio in Drosophila melanogaster
… development is driven by maternally loaded mRNAs synthesized in the female parent during oogenesis. These maternally synthesized mRNAs are regulated by post-transcriptional mechanisms, including mRNA localization, degradation and translational activation and repression. Many of these …
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Investigating the role and regulation of mRNA capping in pluripotency and differentiation
The mRNA cap added to the 5’ end of nascent transcripts is required for the efficient gene expression in eukaryotes. In vertebrates, the guanosine cap is methylated at N7 position by RNMT, which is in complex with its activating subunit RAM. Additionally, the first and second transcribed …
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Regulation of mRNA expression through 3’ UTR sequences of IL6 gene in amino acid-deprived HeLa cells
… responses triggered by amino acid-deprivation of cancer cells activate STAT3 and NF-κB transcriptional factors subsequently to induce IL6 cytokines. Here, it was investigated whether 3’UTR of IL6 mRNA played a role in IL6 induction through cellular stress responses. In the studies with …
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mRNA stability in soma and neurites of cultured neuronal cells
Neurons rely on mRNA localization and local protein synthesis in neurites to regulate synaptic plasticity, axonogenesis and neural signaling. The local regulation of mRNA stability in neurites is poorly understood. To determine mRNA decay rates, we analyzed the subcellular transcriptomes of neural …
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Deconvoluting Cell-Type Specific 3'UTR Isoform Expression in the Adult and Developing Cerebellum
… polyadenylation has been implicated in the regulation of mRNA translation and stability, as well as mRNA and protein localization. However, it is unclear to what extent alternative polyadenylation regulates these processes uniquely in specific cell types. Using a newly developed technique, …
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Single molecule pull-down of viral RNA-protein complexes
… cellular processes such as post-transcriptional regulation of mRNA. Recently, a single molecule pull-down (SiMPull) assay was developed to isolate and study single protein complexes directly from cell lysates. Unlike ensemble measurements, SiMPull is a powerful tool that allows detection of …
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mRNA localization in Caenorhabditis elegans embryogenesis
… regulating protein output, post-transcriptional regulation of mRNA is essential for life. As a result, many mechanisms underlying post-transcriptional regulation are highly conserved across the kingdoms of life. As the spatial resolution of microscopy and sequencing assays has increased, mRNA …
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Sexually dimorphic Argonaute localization underlies sex-specificity of small RNA pathways during germ cell development
Meiosis, a specialized form of cell division that produces haploid gametes, requires precise gene regulation to mediate proper germ cell development. Utilized during both oogenesis and spermatogenesis, small RNA pathways tune expression during germ cell development using functional Argonaute (AGO) …
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Post-Transcriptional Regulation of Mammalian Gene Expression In Non-Coding Region of Target Rna
… in the human chromosome 3p21.3 region. TUSC2 mRNA transcripts could be detected on Northern blots in both normal lung and some lung cancer cell lines, but no endogenous TUSC2 protein could be detected in a majority of lung cancer cell lines. Mechanisms regulating TUSC2 protein expression and …
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Investigating the role of RNA 5- methylcytosine on ribosomal and transfer RNAs in Arabidopsis thaliana
… cell and multicellular organisms, including regulation of mRNA translation and plant growth. However, there are still many questions waiting to be answered. In my research, I addressed the roles of m5C on rRNAs and tRNAs in Arabidopsis thaliana. In Chapter 2, NOP2, a putative rRNA m5C …
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Polysomes but not translation are required for estradiol-mediated stabilization of Xenopus laevis vitellogenin mRNA
The role of translation in the estrogen mediated stabilization of Xenopus laevis vitellogenin mRNA was examined. Using a method developed for total liver polysome preparation, the efficiency of translation of vitellogenin mRNA was determined by examining the distribution of the vitellogenin …
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Cell tropism of maedi-visna virus
Maedi-visna virus (MVV) is a lentivirus of sheep, mainly affecting the lungs (maedi) and the CNS (visna). The primary target cells of MVV infection in vivo are cells of the monocyte/macrophage lineage. However, certain MVV strains can infect various other cell types in vitro and by experimental …
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RNA Dynamics in T Cell Activation
… programs in response to external signals. Regulation of messenger RNA and microRNA by RNA-binding proteins is critical to the control of this process. Hence, disruptions of RNA regulatory processes result in neurologic diseases, cancer, and immunologic disorders among other complications. …
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The role of MK2/TTP pathway in regulating early development and innate immunity in zebrafish
The origin of novel structures and the genes that regulate their formation is a challenging question that has been difficult to address. One such novel structure is the yolk syncytial layer (YSL) in teleost fishes like zebrafish. I studied the genetic pathways operating in the YSL using the betty …
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Differential regulation and functions of the ZFP36-family RNA binding proteins in CD8⁺ memory T cells
… cells via cytotoxicity and the production of pro inflammatory cytokines and chemokines. In addition to antigen-specific responses mediated by the TCR, pro-inflammatory cytokines such as IL-12 and IL-18 can induce antigen-independent bystander activation of memory T cells. The ZFP36-family …
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A link between c. Elegans morphogenesis and mrna export
… explored the mechanisms governing development of the Caenorhabditis elegans pharynx. In this study we used whole genome sequencing (WGS) and CloudMap/Hawaiian Variant Mapping to specifically map a mutation that produced a Pun pharynx (unattached pharynx) phenotype. We discovered a …
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Investigations of cellular stress-responsive proteins and PGF gene expression in human trophoblast
… in preeclampsia (PE), a leading cause of maternal morbidity and mortality. The pathophysiology of PE is thought to be manifested by a poorly perfused placenta hampered by hypoxic stress. Two stress-mediate angiogenic responses include post-transcriptional regulation of mRNA stability, …
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A Functional Genomics Approach to the Plant Soluble Pyrophosphatase Family
Although the activities of soluble pyrophosphatases were shown to be essential for a number of prokaryotes and eukaryotes, plants were assumed to lack cytoplasmic soluble pyrophosphatase activity and vacuolar membrane bound proton-pumping pyrophosphatase was accepted as the only enzyme responsible …
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Programming Molecular Devices using Nucleic Acid Hairpins
<p>Nucleic Acid hairpins have been a subject of study for the last four decades. They are composed of single strand that is </p><p>hybridized to itself, and the central section forming an unhybridized loop. In nature, they stabilize single stranded RNA, serve as nucleation</p><p>sites for RNA …
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