Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"reference genes"”.
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A Functional Analysis of microRNAs in Nicotiana tabacum
… in all tissue types tested. Secondly, very few reference gene analyses have been performed in tobacco, therefore a reference gene analysis in tobacco was conducted. In this analysis, 12 housekeeping genes were tested for their effectiveness in serving as reference genes for gene expression …
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The Role of Lysophosphatidylcholine Acyltransferase (LPCAT) 3 in Macrophage Inflammatory Responses
… bacteria, are key factors in the pathogenesis of sepsis. The activation of macrophages with LPS results in rapid changes in the expression of genes encoding cytokines and other inflammatory mediators. The excessive, prolonged and unregulated activation of macrophages in sepsis leads to …
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Expression of biofilm associated genes in Staphylococcus pseudintermedius
… test including the identification of suitable reference genes in this species. Subsequently, that test was used to examine the expression of multiple biofilm associated genes responsible for numerous aspect of biofilm development and maturation. Significant variations in expression levels …
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A Study of Machine Learning Approaches for Biomedical Signal Processing
… be rescaled based on consistently expressed reference genes. However, for normalizing biologically diverse samples, the most commonly used reference genes have exhibited striking expression variability, and distribution-based approaches can be problematic when differentially expressed genes …
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Thyroid hormone action and disruption in the brain: Xenopus as a model to study disruption of thyroid hormone availability on early brain development
… brains showed significant changes in TH related genes including receptors, deiodinases and neural differentiation markers. Immunohistochemistry confirmed the mixture exposure action on neural proliferation as well as neuron and oligodendrocyte size. Mixture exposed tadpoles also displayed …
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Meccanismi fisiologico-molecolari di germinazione in specie spontanee
… in its maintenance. Five probable housekeeping genes, useful as reference genes for quantitative PCR (qPCR) analysis in seed tissue, were identified in Chrysanthemum coronarium and in Leucanthemum vulgare, isolated and confirmed with comparative analysis with homologous genes in model species. …
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Evaluation of Endothelial Cell Responses to Elevated Glucose
… disease. Next, activity of potential reference genes B2M, HPRT1, and ACTB was assessed. All genes were found to exceed acceptable variability, so the E-ΔC T method of data analysis was selected. Next, cellular responses to high glucose treatment at 10.5 mM glucose and 25.5 mM glucose …
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An evaluation of genetic markers for forensic identification of human body fluids
… fluids, and are prone to contamination. Various genes are expressed in different body fluids that could be used as genetic markers for body fluid identification, and are used in forensic investigations. The aim of this study was to use mRNA markers to identify human body fluids, which included …
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Massive Exchange of mRNA between a Parasitic Plant and its Hosts
… generated from each tissue were mapped to host reference genes to distinguish host sequences, and the remaining sequences were used in a de novo assembly of a Cuscuta transcriptome. This analysis revealed that thousands of different Arabidopsis transcripts, representing nearly half of the …
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Breeding and transgenic approaches to improving water use efficiency in white clover (Trifolium repens L.) : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Plant Biology at Massey University, Palmerston North, New Zealand
… of the Tien Shan ecotype with respect to the reference genes, TrActin and TrGAPDH. Further, in the high biomass cv. Grasslands Kopu II, expression of TrNCED1 decreased in the apical and FFEL tissues of plants experiencing a water deficit, and decreased while for the dryland ecotype Tien Shan, …
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Immune gene expression and diversity in relation to gastrointestinal parasite burden in small mammals
MHC genes encode proteins that are responsible for the recognition of foreign antigens and the triggering of a subsequent, adequate immune response of the organism. Thus they hold a key position in the immune system of vertebrates. It is believed that the extraordinary genetic diversity of MHC …
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Defining the ecological and physiological traits of phytoplankton across marine ecosystems
… approach was designed to identify stable reference genes from high-throughput sequence data for use in RT-qPCR assays or metatranscriptome studies. Using a metatranscriptomic approach, the role of resource partitioning in the coexistence of two closely related diatom species in an …
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Biological activity of anthocyanins and their phenolic degradation products and metabolites in human vascular endothelial cells
… for cell stimulation conditions and qPCR reference genes). Differential bioactivity of parent anthocyanins and their phenolic degradants was observed at physiologically relevant concentrations, as only anthocyanins upregulated eNOS expression (by 4- to 7-fold; p < 0.01), whereas both …
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Defining the ecological and physiological traits of phytoplankton across marine ecosystem
… approach was designed to identify stable reference genes from high-throughput sequence data for use in RT-qPCR assays or metatranscriptome studies. Using a metatranscriptomic approach, the role of resource partitioning in the coexistence of two closely related diatom species in an …
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Quantitative Real-Time PCR Analysis of Colchicine Pathway and Rhizome Developmental Genes
… of G. superba sequences have identified putative genes potentially involved in the colchicine biosynthesis pathway and rhizome development, respectively. Using quantitative real-time polymerase chain reaction (qRT-PCR), a comparative analysis of gene expression in G. superba may reveal …
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Transkriptomická analýza bakterií produkujících sloučeniny s přidanou hodnotou
Produkce látek s přidanou hodnotou pomocí mikroorganismů, jako jsou biopaliva nebo biopolymery, je často omezena nedostatečným porozuměním regulačních a metabolických mechanismů, které tyto procesy řídí. Tato práce se zaměřuje na studium dynamiky genové exprese u dvou biotechnologicky významných …
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Three Generations, One Future: A Systematic Analysis on Nicotine's Effect across generations in C. elegans.
… With this in mind, the need for suitable reference genes for normalization and reliable interpretations is necessary. We dedicated our last objective to identify reference gene candidates to serve this purpose. Based on results from five statistical approaches (geNorm, NormFinder, …
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The functions of the RNA polymerase II CTD in transcription and RNA processing
… of upstream antisense RNAs in about one hundred reference genes by RNA-Seq analysis.
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Molecular Pathology: Potential Biomarkers For The Detection Of Down Syndrome Pregnancies
… cell-free fetal DNA (cffDNA) to target different genes on chromosomes 21, 18, and 13 to screen for trisomy 21 (T21), trisomy 18 (T18) and trisomy 13 (T13), respectively. PRSS56 and SPINK7 proteins are secreted proteins and found to be highly expressed in the DS fetus placenta. The level of these …
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Quantitative analysis of neuropilin-2 expression in pigmented lesions and melanoma
… becomes widespread. The processes of angiogenesis and lymphangiogenesis as well as changes in the extracellular environment all play significant roles in the expansion of a primary melanoma and ultimately metastasis. Neuropilins, specifically Neuropilin-2 (NRP2), have been found to be …
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