Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 23 for “"reduced representation"”.
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Genomic Reconstruction of the Domestication History of Sinningia speciosa (Lodd.) Hiern, and the Development of a Novel Genotyping Approach
… world from a broader perspective. In such cases, reduced representation approaches like Genotyping-by-Sequencing (GBS) are efficient low-cost alternatives to whole genome resequencing. However, most of these technologies are subject to patent protection, licensing processes, and fees that …
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Developing an Automated Practice Environment and Feedback Engine for Guided Instruction in the Deformable Bodies Course
… and comparing either individual equations or a reduced representation of a sequence of equations with each other to identify and report differences. We conducted a study to evaluate the quality of the feedback provided by the system. We also conducted surveys on usability of the interface. Based …
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Computing Trajectories: Pathways into Computer Science and Programming Experience in the First Year
… science. To manage growth, and increase the representation of women and underrepresented minorities in the field, we must first understand current patterns for participation, and factors that may impact access and persistence. Universities with common first-year engineering programs present …
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A High-Throughput Approach to Uncover Novel Roles of APOBEC2, a Functional Orphan of the AID/APOBEC Family
… Using the same system combined with enhanced Reduced Representation Bisulfite Sequencing (eRRBS) analyses I showed that, unlike the AID/APOBEC family member AID, APOBEC2 does not act as a 5-methyl-C deaminase. Finally, using a combination of biochemical, Chromatin Immunoprecipitation …
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Damage Detection and System Identification using a Wavelet Energy Based Approach
… system in the time domain were converted to a reduced representation of the equations of motion in terms of the DWT and of the DWPT. Finally, the least square error minimization was conducted over the dominant sets to estimate the best estimation of the physical parameters. The second proposed …
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Methods and Mechanisms of DNA Methylation in Development and Disease
… method, called Laser Capture Microdissected-Reduced Representation Bisulfite Sequencing (LCM-RRBS), accurately and reproducibly profiles genome-wide methylation of DNA extracted from microdissected fresh frozen or formalin-fixed paraffin-embedded tissue samples. Using this method, I find that …
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Genome composition of Glycine max and sequence diversity among cultivated and exotic accessions
… heterochromatization. The third study applied a reduced representation sampling strategy to identify SNP markers in the non-repetitive regions of the genome that can distinguish between soybean accessions. The method developed in this study should be generally applicable to other lines of soybean …
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Oil reservoir characterization using ensemble data assimilation
… iterative history matching algorithms. A reduced representation of reservoir's states and parameters using discrete cosine transform is presented to improve the estimation problem and geological consistency of the results. The discrete cosine transform allows for efficient, flexible, and …
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Evolutionary history and taxonomy of the titi monkeys (callicebinae)
… nuclear and mitochondrial loci, > 14,500 bp) and reduced representation, genome-wide double-digest restriction-associated DNA (ddRAD) sequencing. A statistical biogeographical approach was employed to reconstruct the biogeography of Callicebinae and investigate the processes responsible for …
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Molecular mechanisms of acquired gemcitabine resistance in pancreatic cancer
… following gemcitabine selection. By RRBS (Reduced Representation Bisulfite Sequencing) analysis, 845 genes were found to have altered methylation. One of these genes, PDGFD, was further investigated and found to have reversible demethylation at its promoter region in the drug resistant …
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The effects of virus-induced maternal inflammation on methylation patterns in skeletal muscle
… samples were collected from offspring at birth. Reduced representation bisulfite sequencing was used to determine methylation status of individual CpG sites throughout the genome. Differentially methylated CpG sites were used in pathway and gene ontology analysis. Overall frequency of methylated …
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Lagrangian-based Simplification and Feature Estimation for Flow Visualization
… to those applications. There are two different representations of vector field (or flow) data, i.e., the Eulerian representation and the Lagrangian representation. Recently, the Lagrangian-based flow representation is getting increasing attention from the computational fluid dynamics (CFD) …
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Population Structure of Lake Whitefish (Coregonus Clupeaformis) on Multiple Spatial Scales: Transitioning to a Genomics Approach
… polymorphisms in a subset of the genome based on reduced representation DNA libraries (RRL). These tools better represent evolutionary processes on a genomic level but molecular ecologists are now faced with many important and potentially complex decisions about study design. These factors greatly …
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Genomics and ecology of rapid adaptation: the expansion and evolution of the invasive common myna (<i>Acridotheres tristis</i>) and common starling (<i>Sturnus vulgaris</i>) in New Zealand
… myna as the main focal species. Firstly, I used reduced representation sequencing data to identify the origin and structure of myna populations, with emphasis on New Zealand. Two sub-populations in New Zealand were identified, divided by the North Island axial mountain range, suggesting …
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Linkage mapping in tetraploid blackberry (Rubus spp.) using high-throughput genomic sequencing and restriction-site associated DNA sequencing (RAD-seq)
… in tetraploid blackberry (Rubus spp.) using the reduced-representation, restriction-site associated genomic DNA sequencing method; 2) to discover quantitative trait loci (QTL) associated with traits of economic importance to blackberry breeders and producers; and 3) to characterize the structure …
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Genome-Wide Characterization of the Effects of Nucleic Acid Modifying Enzymes: Cytidine Deaminases and DNA Methylation
… of RNA expression and editing, while reduced-representation bisulfite sequencing (RRBS) assays DNA methylation. These analyses confirmed AID's known role in immunoglobulin isotype switching, while also demonstrating that it has little other effect on gene expression. Additionally, no …
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Decoding the regulatory role and epiclonal dynamics of DNA methylation in 1482 breast tumours
… less attention. To address this, an optimised Reduced Representation Bisulfite Sequencing protocol was performed on 1482 primary breast tumours (and 237 matched adjacent normal tissues). This constitutes the largest breast cancer methylome yet, and this thesis describes the bioinformatics and …
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Programming of cardiovascular disease: an exploration of epigenetic mechanisms
… other differences were strain-specific. Enhanced Reduced Representation Bisulfite Sequencing was performed on liver and fetal germ cells from males exposed to Dex in utero to explore effects on DNA methylation. These studies confirm that epigenetic reprogramming occurs in the rat and that this …
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Physiological and Molecular Consequences of Large Y Chromosome Long Arm Deletions in Mice
… a number of multi-copy genes whose absence or reduced representation has been linked to sperm defects and offspring sex ratio distortion in favour of females. Understanding the biological mechanisms of sex ratio distortion and related sperm aberrations could not only result in benefits for …
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Computational methods for genomic variant calling and analysis
… (GBS) analysis methods on plant data. As a reduced-representation sequencing method to identify genetic variants and quickly genotype samples, GBS produces extensive missing data and requires complex bioinformatics analysis, particularly in the context of plants, which have highly variable …
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