Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 111 for “"redox state"”.
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Control of Cellular Redox State and Biomass Synthesis
… undergo oxidation reactions that require the redox cofactor NAD+ as an electron acceptor. Thus, the cellular redox state, measured by the NAD+/NADH ratio, can constrain the synthesis of oxidized biomass. This dissertation aims to uncover the determinants of the cellular NAD+/NADH ratio and how …
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The Influence of Cellular Redox State on Mitosis
… coincides with a robust reduction in cellular redox status, preventing disulfide bond-mediated activation of Aurora kinase B as a determinant of anaphase onset. In cells lacking the ability to alter their redox state due to deficient mitochondrial respiratory function, deficient Aurora kinase B …
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Modulating adipogenesis via signaling pathways and cellular redox state
The findings of this thesis help to understand how nitric oxide (NO) plays a pivotal role in the cell physiology of human progenitor fat cells (preadipocytes). NO significantly promoted the differentiation process from immature fat cells to mature adipocytes. The proliferation of preadipocytes, …
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Effects Of Altering The Peroxisomal Redox State In Models Of Degenerative Disease
… are important regulators of cellular redox balance and function as a signaling platform to regulate anti-aging metabolic and communication networks. In addition the organelle has emerged as a major player in maintaining cellular ROS at an optimal level. At such levels, these ROS are …
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Circadian rhythms of redox state and regulation of neuronal excitability in suprachiasmatic nucleus of rodents
… is sensitive to metabolic (redox) change; however, energetic cycles manifest as circadian rhythms in protein oxidation have been reported in anucleate cells, where no transcription occurs. Whether the brain clock expresses redox cycles and how such metabolic oscillations …
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Yeast Ataxin-2 (Pbp1) Condensates Regulate TORC1 Activity and Autophagy in Response to Cellular Redox State
… the protein into either liquid-like or gel-like states in vitro and enables self-association of full-length Pbp1 into pelletable assemblies in vivo. Sequence analysis revealed that Pbp1 LC region contains an unusually high frequency of methionine residues (24 methionines in 150 a.a.) compared to …
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Design and Development of a Novel Immunological Method to Quantify target-specific cysteine redox state in a microplate
… was showcased in the present<br/>study where the redox state of interleukin 1 receptor associated kinase (IRAK) 1<br/>could not be measured using mobility shift assays. To address this technical<br/>impediment, a novel technique called RedoxiFluor was developed. RedoxiFluor<br/>involves the …
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Spring Forward or Fall Back? Redox State as a Determinant in Glutamate Signaling Pathways Adjusting Our Brain Clock
… phase-resetting response depends on the temporal state of the clock: during the early night, light/glutamate delays clock phase, whereas during the late night, clock phase is advanced. Key signaling elements downstream of glutamate have been identified as ryanodine receptor (RyR) and soluble …
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Genetic Variants in the Putidaredoxin-Cytochrome P-450(cam) Electron Transfer Complex: Identification of the Residue Responsible for Redox-State Dependent Complex Stabilization
… and a two-protein electron transfer chain, putidaredoxin and putidaredoxin reductase (Tyson et al., (1972) J Biol. Chem. 274, 5777-5784). The enzymatic removal of putidaredoxin's C-terminal tryptophan is known to cause a much reduced rate of enzymatic activity in the reconstituted camphor …
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Using High-Resolution NMR to Examine the Components that Contribute to the Activity and Stability of Phosphatase of Regenerating Liver (PRL-1) and Their Dependence on the Redox State of Cysteine Residues
… PRL-1. It is well-established that the oxidation state of cysteine residues in proteins are critical to overall physical stability. The presence of disulfide bonds most often imparts thermodynamic stability, and as such, engineered disulfide bonds have become a means for improving the viability of …
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The role of redox oscillation in circadian physiology of the suprachiasmatic nucleus and hippocampus
… can lead to disruption of function and disease states, such as sleep disorders, metabolic syndromes, and cardiovascular disease. The main driver of circadian behavior is a transcription-translation feedback loop of core circadian genes. Emerging evidence suggest that metabolic oscillators also …
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Characterizing Factors That Influence Intracellular Thiol-Disulfide Equilibrium
… is to maintain proteins in their functional redox state while promoting redox processes required for cell growth.</p> <p>The first part of this thesis focuses on determining the role that the oxidoreductase human glutaredoxin 1(hGrx1) plays in reduction of the critical disulfide bond in human …
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Visualization of energy transfer among redox active polymeric colloids via in-situ fluorescence microscopy
… being considered for that purpose and redox flow batteries are considered one of the most promising designs for stationary energy storage due to their scalability, high-efficiency and decoupled control of energy and power. To prevent the crossover of energy carriers between anolyte and …
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Glutamine Phosphoribosyl Pyrophosphate Amidotransferase From Bacillus Subtilis: Iron-Sulfur Biochemistry and Enzymology
… enzyme, the cluster exists in the diamagnetic +2 redox state. Treatment of the cluster with oxidants resulted either in no reaction or in oxidative dissolution of the cluster, i.e., a +3 state was not detected. The cluster was reduced poorly with sodium dithionite, but was reduced readily by …
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Increasing Heterologous Protein Secretion From the Yeast S. Cerevisiae Through Manipulation of Cellular Redox Factors
… in this thesis involves the effect of cellular redox factors on BPTI secretion. Glutathione levels and redox state were altered in an attempt to determine if optimal BPTI secretion requires a certain total glutathione level and/or redox state. Evidence for an optimal glutathione level that …
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Investigating Subcellular Thiol Redox Chemistry With Gfp-Based Redox Sensors
… (GSH and GSSG), which serve as an intracellular redox buffer. In order to better understand the factors that influence mitochondrial GSH:GSSG balance, we used genetic engineering methods to target fluorescent protein-based redox sensors to the matrix and intermembrane space (IMS) of yeast …
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Metabolic flux, transport activity, and subcellular communication in intact hearts from dynamic ¹³C NMR spectroscopy
… at normal (no lactate) or high cytosolic redox state (2.5 mM lactate). High cytosolic redox state showed little effect on substrate utilization and TCA cycle activity. The TCA cycle flux was 9.7±0.5 [mu]moles/min/g dry weight with butyrate only and 10.2±0.3 with butyrate plus lactate. …
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Regulation of the stomatal response to red light
… One current hypothesis is that the redox state of the chloroplast plastoquinone (PQ) pool could coordinate the Ci-independent response to red light. However, the exact site of perception, and the mechanisms and cell types through which the red light stimulus is communicated remain …
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Redox manipulation alters hippocampal neuron response to semaphorin 3A
… known as semaphorin 3a (Sema3A) and various redox reactions such as the oxidizing function of Mical proteins on f-actin that function downstream of Sema3A. However, what is less understood is how various redox conditions alter these guiding cues. This work investigates the subcellular redox …
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