Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 348 for “"reconstitution"”.
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A Reconstitution of Place
By drawing upon the essential characteristics of a locality, an architect may, through reinterpretation, reconstitute a place. This occurs when mundane characteristics of a locality are reformulated in such a manner that their presence becomes significant.
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Stepwise Reconstitution of RNA Cellular Activity
… predictive of viral functions in cells. Reconstitution, in which a cellular process is reduced to the in vitro behavior of its component parts, is a promising strategy for establishing such predictive relationships, but there is debate as to whether the structures and activities of RNAs …
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Immune reconstitution post bone marrow transplantation
… project were therefore: to document the immune reconstitution following T-cell depleted bone marrow and peripheral blood stem cell transplantation and to compare this with the recovery following autologous grafts. to document the cell surface expression of CD95 in an attempt to comment on the …
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The reconstitution of the histone octamer
This thesis describes methodology for the reconstitution of the chicken erythrocyte octamer from acid-denatured histones or the natural H3-H4 tetramer and H2A-H2B dimers. Oligomeric properties of reconstituted octamers were elucidated during column chromatographic and chemical cross-linking …
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Reconstitution of Munc18-Dependent Mast Cell Degranulation
… SNAREs using <em>in vitro</em> reconstitution. We showed that Munc18a was active in stimulating fusion mediated by VAMP2 and VAMP3, but not by VAMP7 and VAMP8. In contrast, Munc18b and Munc18c did not show any stimulatory activity. Further analysis of Munc18a function unraveled a …
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In vitro reconstitution of Central Spindle Motility
… a combination of in vivo imaging and in vitro reconstitution using purified proteins and micropatterning. To address how different endosomes segregate on an asymmetric microtubule track I utilised knock-in Rab-GFP lines and internalised labelled anti-delta antibodies. Remarkably, a class of …
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Reconstitution and Characterization of Human Endogenous Retrovirus-K
<p>Retroviruses are a family of clinically significant and scientifically fascinating viruses that infect a wide array of organisms from all vertebrate classes. The two hallmark events in the life cycle of retroviruses are the reverse transcription of the single stranded RNA (ssRNA) genome …
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Reconstitution Of Rho Gtpase Interactions At The Membrane
In order for fibroblasts to migrate, budding yeast to polarize, and macrophages to undergo phagocytosis, each cell must coordinate a membrane-localized signal with a robust morphological change. The Rho Family of GTPases play critically important roles in mediating these cellular processes. …
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Synthetic In Vivo Reconstitution of Asymmetric Cell Division
… be untangled. This represents the most complete reconstitution of asymmetric cell division in symmetrically dividing, unpolarised cells to date. This is the first demonstration that an asymmetric Par complex is sufficient to reinstate polarised signalling and important aspects of ACD in …
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Characterization and Reconstitution of S-Palmitoylated IFITM3 Antiviral Activity
<p>To rapidly detect early stage infections the innate immune system maintains an assortment of pathogen recognition mechanisms interspersed throughout both the extracellular and intracellular environments. These sensors recognize key components of viral, bacterial and fungal pathogens, and …
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Biochemical Reconstitution and Functional Characterization of the WAVE Regulatory Complex
Members of the Wiskott-Aldrich syndrome protein (WASP) family (WASP, N-WASP, WAVE 1-3) have a central role in the transmission of the extracellular signals to the actin cytoskeleton. These proteins use their C-terminal VCA domain to stimulate the actin-nucleating activity of Arp2/3 complex in …
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Reconstitution and Mechanistic Studies on the Staphylococcal agr Quorum Sensing Circuit
… exhibits several remarkable properties in our reconstitution system based on nanometer-scale lipid-bilayer discs (nanodiscs). Activation of this receptor by its native activator, for instance, requires a membrane environment enriched of anionic lipids mimicking the electrostatic property of the …
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Prolonged tuberculosis-associated immune reconstitution inflammatory syndrome: characteristics and risk factors
… tuberculosis who develop paradoxical immune reconstitution inflammatory syndrome (IRIS), the clinical course of IRIS is prolonged necessitating substantial health care utilization for diagnostic and therapeutic interventions. This phenomenon has not been systematically studied to date. We …
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Immunological characterization of the HIV-tuberculosis associated immune reconstitution inflammatory syndrome
… of patients, HIV-Tuberculosis associated Immune Reconstitution Inflammatory Syndrome (TB-IRIS) has been shown to occur in a significant subset of these patients. TB-IRIS is an inflammatory complication of the combined treatments for HIV-1 and tuberculosis, which is being reported increasingly, …
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Investigating the molecular pathogenesis of tuberculosis-associated immune reconstitution inflammatory syndrome
… of paradoxical tuberculosis associated immune reconstitution inflammatory syndrome (TB-IRIS). Paradoxical TB-IRIS is an aberrant immune response targeted against Mycobacterium tuberculosis or its residual antigens in patients with advanced human immunodeficiency virus (HIV) infection shortly …
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Prostate Cancer Stem Cells: Drug to lerance, Experimental Reconstitution and Castration Resistance
<p>Prostate cancer (PCa) is one of the leading malignancies affecting men in the Western world. Although tremendous effort has been made towards understanding PCa development and developing clinical treatments in the past decades, the exact mechanisms of PCa are still not clearly understood. …
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In vitro reconstitution of the ubiquitylation and disassembly of the eukaryotic replisome
… CMG helicase in vitro. My work resulting in the reconstitution of CMG disassembly in vitro is the first example of the disassembly of a multi-subunit physiological substrate of Cdc48.<br/><br/>Though CMG is ubiquitylated in yeast extracts in vitro, it does not lead to its disassembly and …
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