Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"recombinational repair"”.
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The role of recombinational repair in the mechanism of killing during thymine starvation in Escherichia coli
… formation, and characterization of the role of recombinational repair in preventing, repairing or generating these chromosomal lesions. I first characterized the extent of DNA replication, stability and damage during TLD using physical methods, such as pulse-field gel electrophoresis to measure …
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Genetic requirements for protection against bleomycin toxicity in Escherichia coli
… Escherichia coli strain to a panel of isogenic repair deficient mutants. If the pathway defective in the mutant is known (e.g., base excision repair, alkyl transferase repair, nucleotide excision repair, and so on), the sensitivity of the mutant can reveal mechanistic insight into the mode of …
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The molecular bases of tryptophan replacement effects in RecA: A biochemical and biophysical characterization of single- and null- tryptophan mutant Escherichia coli RecA proteins
Recombinational DNA repair is an essential component of DNA metabolism and the proteins required for recombinational repair pathways are highly conserved. The Escherichia coli RecA protein is studied as a prototypic strand exchange protein with structural and biochemical similarity to the archaeal …
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The Rad51 family of proteins: Interactions, vitamin D, and implications in head and neck cancer
… non-homologous end-joining (NHEJ) or homologous recombinational repair. Five human proteins with homology to Rad51 known as the Rad51 paralogs, Rad51B, Rad51C, Rad51D, XRCC2, and XRCC3, whose loss of function in cell lines leads to high chromosomal instability. Previous studies have shown Rad51C …
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The role of mismatch repair in mediating cellular sensitivity to cisplatin : the Escherichia coli methyl-directed repair paradigm
… paradox in the field is how loss of mismatch DNA repair leads to clinical resistance to this widely used drug. The phenomenon of cisplatin tolerance in mismatch repair deficient cells was initially discovered in E. coli, where methylation deficient dam mutants show high sensitivity to cisplatin …
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Repair of DNA-protein crosslinks in mammalian cells
… chain reaction (SSPE-qPCR) used to study the repair of DNA-protein crosslinks in mammalian cells. DNA-protein crosslinks (DPCs) are bulky lesions which disrupt important cell processes such as transcription and replication. They are formed by endogenous molecules such as formaldehyde and …
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Analysis and confirmation of the results of a yeast two-hybrid screen carried out to identify proteins that interact with drosophila XRCC2
<p>Repairing DNA damage is brought about by highly specific proteins that partake in a variety of DNA repair. Two of the most common types of damage are double-strand breaks (DSBs) and interstrand crosslinks. A single DSB or crosslink can potentially kill a cell if it is not repaired~ In human and …
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The iron-dependent cyanide and hydrogen peroxide co-toxicity in Escherichia coli and its catastrophic consequences for the chromosome
… microbes. We also showed that the base excision repair pathway plays the major role in preventing this catastrophic chromosomal fragmentation, which indicates that the double-strand breaks induced by CN + H2O2 are preceded by a significant number of base modifications, removed by base-excision …
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Study of mechanism of genetic death in Escherichia coli in response to A) dTTP starvation and B) combination of temperature sensitive defects in replication initiation and double strand break repair
… initiation and recBC, in double-strand-break repair. This lethality was discovered by Sharik Kahn, a Post-Doctoral researcher in this lab, and I continued to investigate this mysterious synthetic lethal combination. The first chapter presents a review of the phenomenon of TLD, the history of …
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Rate zonal density gradient ultracentrifugation analysis of repair of radiation damage to the folded chromosome of Escherichia coli
… direct measurement of the formation and repair of DNA double-strand breaks at biologically-significant doses of ionizing radiation. In vitro UV-irradiation of nucleoids resulted in an increase in the observed rate of sedimentation due to the formation of an unknown photo-product. In …
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Role of XRCC3 in Acquisition and Maintenance of Invasiveness through Extracellular Matrix in Breast Cancer Progression
… that collaboratively assist RAD51 in homologous recombinational repair (HRR) of DNA double-strand breaks. To see if these interactions are important in terms of invasion, as they have been demonstrated for DNA repair, we studied the effect of XRCC3 downregulation on the levels of RAD51 paralogs. …
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Investigation of The Roles of Asf1 and Caf-1-Mediated Chromatin Assembly In The Human Dna Damage Response
<p>The access-repair-restore model for the role of chromatin in DNA repair infers that chromatin is a mere obstacle to DNA repair. However, here we show that blocking chromatin assembly of newly-synthesized histones, via knockdown of the histone chaperones ASF1A, CAF-1 or a mutation that …