Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 32 for “"recombinant protein expression"”.
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Overcoming long-standing challenges in recombinant protein expression
<p>The market for proteins has been constantly increasing for many different applications, such as protein-based drugs. However, protein production in E. coli, the most widely used recombinant host, has remained fundamentally the same since its inception. Here, we developed from the bottom up, a …
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High-throughput experimentation for microscale cultivations and recombinant protein expression
… profound understanding and hence optimization of recombinant expression systems and high-throughput cultivation processes. New concepts to deal with issues of different growth kinetics of cultures in microtiter plates are elaborated and tested. Furthermore, a novel high-throughput experimentation …
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THERMOSTABLE EXOSHELLS AS TOOLS FOR RECOMBINANT PROTEIN EXPRESSION AND IN VIVO DELIVERY
We have engineered a thermostable protein nanoparticle (tES) to improve both expression and stabilization of recombinant proteins using a single technology. tES provides steric accommodation and charge complementation to GFPuv, horseradish peroxidase (HRP), and Renilla luciferase, and Photinus …
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Molecular cloning, recombinant protein expression and structural characterization of a universal stress G4LZI3 protein from Schistosoma mansoni
… has been predicted to encode different stress proteins including the Universal Stress Protein (USP). It has been previously hypothesized that the USP of schistosome parasites have shared protein sequence features which can be useful for inferring their biochemical and environmental regulation. …
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Characterisation of an expression system for commercial production of proteins / by Lene Jorgensen.
… of this thesis is to characterise a bacterial expression system for recombinant production of proteins with relevance to industry. Recombinant protein expression under control of stationary-phase inducible promoters is characterised, and the expression levels are quantitatively compared with …
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Optimisation of the mycobacterial replicon of an E. coli-mycobacterial shuttle vector
… repB genes which code for essential replication proteins. Specific aspects of this typical mycobacterial replicon were identified as potential targets to improve stability and increase recombinant protein expression levels, and were modified accordingly.
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Development of microalgae as a biopharming platform
… microalgae transient production platform for the expression of recombinant protein. First it had to be established whether the available plant expression vectors could be used for the transfer of genetic material into packed microalgae. The method showed successful transfer of the neomycin …
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Enhancement of plant expression vectors using replication and silencing suppressor elements
… traction as a cost-effective platform to produce recombinant proteins. Further improvements can be made, however, to increase overall yield especially for difficult to express proteins. In this study virus-derived silencing suppressors and replication elements were used with the aim of increasing …
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Structural and Functional Evolution of Human Heat Shock Transcription Factors
… Transcription Factors (HSFs) that regulate the expression of numerous genes involved in protein folding, protein degradation, and cellular survival. The studies presented here utilize a diverse array of techniques including yeast genetics, recombinant protein expression and purification, …
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Engineering and use of plant viral expression vectors
Different plant virus-based expression systems were developed and compared using the colorectal cancer antibody CO17-1A and the corresponding antigen GA733-2 as model proteins. Tobacco mosaic virus (TMV) and alfalfa mosaic virus (AlMV) were used to construct the vector backbones of four systems to …
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Development of a codon-optimized Latrodectus hesperus MaSp1 synthetic gene for bacterial protein expression using a seamless cloning strategy
… codon-optimized MaSp1 minifibroin constructs for recombinant protein expression in bacteria. These recombinant MaSp1 minifibroin constructs were engineered to contain the N-terminal domain (NTD), different copies of internal block repeats (ranging from 2 to 64 copies of 35 amino acid blocks), and …
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The effects of cis- and trans-acting mutations on recombinant protein secretion in Pichia pastoris
… in both research and industrial settings for recombinant protein expression due to the ease of genetically modifying its genome, its ability to grow to large densities in inexpensive media, and its capability to perform posttranslational modifications. Multiple tools such as the cis -acting …
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Genomic and Proteomic Characterisation of the European House Dust Mite, Dermatophagoides pteronyssinus
… data and limited to a discrete number of proteins. The work presented here is a detailed characterisation of the European house dust mite, Dermatophagoides pteronyssinus airmid strain, at the genomic and proteomic level. Genomic sequencing and assembly resulted in a high-quality assembly …
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Molecular characterisation of the "LEAome" in the resurrection plant Xerophyta humilis (Baker)
… shown that Late Embryogenesis Abundant (LEA) proteins are expressed upon the onset of desiccation and are therefore inferred to be associated with the desiccation tolerance response. To date, despite some 25 years of research on these proteins, there is still very little understanding of the …
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Characterisation of a protease-dependent gene regulation system for gene therapy applications
… active promoters to drive high levels of recombinant protein expression. Unregulated gene expression is a key issue limiting clinical translation of promising gene therapies due to the potential for side-effects from excessive levels of protein that indiscriminately affect both diseased …
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Investigating the Role of Trimeric Autotransporter Adhesins in Fusobacterium nucleatum Pathogenesis
… available to functionally analyze F. nucleatum proteins. Interestingly, F. nucleatum has a diverse landscape of structurally unique surface adhesins called Type 5c secreted trimeric autotransporter adhesins (TAAs), which are a family of proteins that are historically known for their …
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Examination of 2-Oxoglutarate Dependant Dioxygenases Leading to the Production of Flavonols in <i>Arabidopsis thaliana</i>
… this goal, an activity assay employing recombinant protein expression and HPLC as a detection system was developed for F3H and adapted for use with FLS. The assay was employed to establish the biochemical parameters of F3H from Arabidopsis, and to further characterize the F3H mutant …
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Development of a Cytochrome P450 Expression System with Biomass Scale-up, Streamlined Downstream Processing and Stability Modifications
Recombinant human P450s are important in preclinical studies to discover possible interactions between drugs when taking medicines. Drugs may be either inhibitors or substrates for P450s. Some P450s also play a major role in cancer and other diseases. Recombinant P450s offer better activity, …
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Cell cycle checkpoints and checkpoint kinases in the apicomplexan parasites Plasmodium and Toxoplasma
… control in model eukaryotes. Members of this protein family are missing in *Plasmodium*, but some remain in the close relative *Toxoplasma gondii*. A suite of experiments were conducted to investigate these potential checkpoints, including cellular assays on the effect of PIKK-inhibiting drugs …
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Bioprospecting novel hemicellulases from the gut microbiota of the common black slug (Arion ater)
… The conserved domains and related proteins were identified using various bioinformatic tools. The protein structure was developed with the homology modelling tool hosted in I-Tasser. Candidate genes were PCR amplified and cloned into expression vectors for recombinant protein …
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