Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 17 of 17 for “"recombinant plasmids"”.
-
Investigation of the Substrate Recognition Characteristics and Kinetics of Mammalian Mitochondrial DNA Topoisomerase I
… methods for PCR-cloning. These mtDNA containing recombinant plasmids were used in a kinetic analysis of the highly purified enzyme. Recombinant plasmids containing the bovine mtDNA heavy and light strand origins of replication (pZT-Hori and pZT-Lori, respectively), a major transcription …
-
Gene cloning studies in two nocardioform bacteria
… derived from pEcoR251, thereby selecting for recombinant plasmids containing inserted DNA. The thiostrepton resistance gene derived from pIJ702 was shown to be functional in Streptomyces lividans enabling selection of recombinant pLR591 plasmids containing foreign DNA in S. lividans. The …
-
ΜΕΛΕΤΗ ΤΩΝ ΦΥΣΙΚΩΝ ΠΛΑΣΜΙΔΙΩΝ ΤΟΥ ΣΤΕΛΕΧΟΥΣ ATCC 10988 ΤΟΥ ΑΙΘΑΝΟΛΟΠΑΡΑΓΩΓΟΥ ΒΑΚΤΗΡΙΟΥ ZYMOMONAS MOBILIS
FIVE NATIVE PLASMIDS OF Z. MOBILIS STRAIN ATCC 10988 HAVE BEEN ISOLATED, NAMELYPZMO2, PZMO3, PZMO5, PZMO6 WITH SIZE 1,70, 1,85, 2,70, 16,7, AND 31,8 KB RESPECTIVELY. RESTRICTION MAPPING (PARTIAL) OF ALL THESE PLASMIDS WAS FOLLOWED AND THEIR HOMOLOGY LEVELS WERE DETERMINED. SIMILARLY, THEIR HOMOLOGY …
-
Studies on nucleotide levels and electron transport genes of Clostridium acetobutylicum P262
… for the isolation of C. acetobutylicum genes on recombinant plasmids which activated metronidazole. Twenty-five E. coli F19 clones which contained different recombinant plasmids were isolated. The clones were tested for nitroreductase, pyruvate-Fdoxidoreductase and hydrogenase activities. …
-
Developmental genetic studies on Thiobacillus ferrooxidans
… for plasmid DNA transfer to T. ferrooxidans. Recombinant T. ferrooxidans plasmids, pDER401 and pDER405, were shown to code for mobilization and replication functions in Escherichia coli and Thiobacillus novellus strains. The plasmids were mobilizable at high frequency by the IncP plasmid, …
-
Cloning of actin genes from a genomic library of the newt, Notophthalmus viridescens
… gene for a molecular probe. Two independent recombinant phage clones, containing actin homologous inserts, were isolated from a newt genomic library by hybridization with the chick actin probe. Restriction mapping identified actin homologous sequences within the newt DNA inserts which were …
-
Nucleotides in yeast phenylanyl - transfer-RNA required for the specific recognition by its cognate yeast phenylalanyl - transfer-RNA synthetase
… developed. Using synthetic DNA oligonucleotides, recombinant plasmids were constructed with a T7 promoter directly upstream of the tRNA gene and a BstN 1 restriction site at the 3$\sp\prime$ terminus of the gene. Run-off transcription of the wild type plasmid gave a 76 nucleotide RNA having the …
-
Structural Analysis of the TOL pDK1 xylGFJQK Region and Partial Characterization of the xylF and xylG Gene Products
TOL plasmids encode enzymes responsible for utilization of toluene and related aromatic compounds by Pseudomonas putida, ultimately converting them to central metabolic intermediates. The nucleotide sequence for the 5.6 kb xylGFJQK region of the pDK1 TOL meta operon was determined. DNA sequence …
-
Isolation And Characterization Of Human Ribosomal Protein Complementary-dnas
… with these probes permitted the isolation of recombinant plasmids whose cDNA inserts encompass the entire coding sequence of S6 (249 aa residues), 27 bp of the 5{dollar}spprime{dollar} untranslated leader and all 39 bq of the 3{dollar}spprime{dollar} untranslated region. A comparison of the …
-
Mapping the Major Epitope(s) of the Glycoprotein of Ebolavirus with QB Phage Display System
… its A1 the readthrough minor coat protein. The recombinant phages would then be utilized to analyze their antigenicity and to map the major epitope(s) of the glycoprotein determinants. This research study represents a proof of concept and will serve as a guide as to how to produce recombinant …
-
A molecular biological study on Campylobacter pylori
… be plasmid mediated, it was decided to study the plasmids of C. pylori. A variety of techniques were used to establish the best method of plasmid extraction from C. pylori. The method of alkaline lysis as described by Birnboim and Doly was shown to give the most consistent results and the greatest …
-
Plasmid stability of pUB110 and pUB110-derived plasmids in Bacillus sphaericus 2362
… cell division) of this plasmid and four plasmids derived from pUB110 was examined. The recombinant plasmids were developed in the laboratory of Dr. William Burke at Arizona State University. Stability was examined under various conditions of cell growth. Throughout spore germination, …
-
Cloning and expression of a functionally active truncated N-glycosylated KSHV complement regulatory protein and immunohistochemical studies with the anti-KCP peptide antibody
… the expression vector pPIC9 to generate three recombinant plasmids for heterologous expression in the yeast, Pichia pastoris and to produce separately, the 4 N-terminal Sushi domains (KCP-S, small), KCP protein lacking the putative transmembrane binding domain (KCP-M, medium) and the …
-
The characterisation of a novel Xanthomonas Bacteriophage
… extrachromosomal element was cloned and several recombinant plasmids sequenced. No typical phage genes were identified. However, fragments of a Xanthomonas avirulence (avr) gene interrupted by part of a transposon sequence were identified. A 201- 262 base region of clone pSSI shared a 98% …
-
Development of West Nile virus candidate vaccines in Nicotiana benthamiana
… and envelope (E) proteins. Infiltration of these recombinant plasmids into plants yielded no protein expression unless co-expressed with the human chaperone protein calnexin (CNX), upon which expression of both M and E proteins were observed. We investigated the assembly of prM and E into VLPs by …
-
Developing novel SNAP tag-based antibody fusion proteins directionally conjugated to fluorophores for immunophenotyping of acute myeloid leukemia
… with a SNAP tag, which can be readily produced recombinantly under cost-controlling conditions. SNAP-tag is a modified version of the human DNA repair enzyme alkylguanine-DNA alkyltransferase (AGT), which reacts specifically with O6-benzylguanine (BG)-modified molecules via irreversible transfer …
-
Cloning of a <i>CHLAMYDOMONAS REINHARDTII</i> Marker into a RNA Interference Construct to Test Whether the Photoreceptor Chlamyrhodopsin Is Involved in Circadian Clock Resetting
… it and transformed into <i>E.coli</i>. The recombinant clones obtained were confirmed by restriction digests. Fusion regions and the orientation of the insert in the recombinant plasmid were confirmed by sequencing. An attempt was made to transform <i>C. reinhardtii </i> with the construct, …