Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"recombinant enzyme"”.
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Studies on cathepsin B of Eimeria tenella and pyroglutamyl peptidase of Leishmania major
… and did not carry any introns. The E. tenella enzyme, sharing 42% identity with the Toxoplasma gondii toxopain-1, which is reportedly involved in the invasion of host cells, was expressed as a soluble inactive zymogen in E. coli. Recombinant cathepsin B of E. tenella was functionally expressed …
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Improvement of engineered thermostable xylanase production via immobilization of recombinant escherichia coli onto graphene oxide
… coli is the preference host system for enzyme production by recombinant DNA technology. This was due to its promising trait that is suitable for genetically modify and the obtainability of a difference E. coli strains. But, the main downside when utilizing E. coli as a host is bacterial …
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Action of Tyrosyl DNA Phosphodiesterase on 3'-Phosphoglycolate Terminated DNA Strand Breaks
… and western blotting codmed that the putative enzyme in human extracts that efficiently processed PG was indeed tyrosyl DNA-phosphodiesterase. When recombinant hTdpl was purified using HiTrap nickel chelating columns and its PG processing activity compared to that of partially purified native …
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Overproduction of xylose isomerase in recombinant Escherichia coli
Aspects of recombinant enzyme production were characterized in genetically engineered Escherichia coli. Five strains transformed with the xylose isomerase overproduction system (pRK248/pTXI-1) were compared, based on parameters of cell metabolism and inducible enzyme activity in shake flask …
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Protein and cell therapy for lecithin-cholesterol acyltransferase (LCAT) deficiency
Lecithin-cholesterol acyltransferase (LCAT) is an enzyme principally secreted by the liver into the circulation where it esterifies cholesterol and plays a key role in high- density lipoprotein (HDL) metabolism. In familial and acquired (liver disease) LCAT deficiency, the failure to esterify …
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The Role of S-Adenosylmethionine Decarboxylase on Regulation of Polyamine and Trypanothione Metabolism in Trypanosoma Brucei
… is essential for T. brucei, and the biosynthetic enzymes in this pathway are potential drug targets. We have characterized T. brucei S-adenosylmethionine decarboxylase (AdoMetDC), a key enzyme required for the synthesis of spermidine and trypanothione, and examined the role of AdoMetDC on the …
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Two Methodologies in Pursuit of the Elucidation of Copper (II)—Centered Bioinorganic Chemistry
… first method explored involves the promiscuous enzyme Streptomyces griseus aminopeptidase (SgAP) which although known primarily as a peptidase has been shown to oxidize catechol under near physiological conditions in vitro when its native ZnII ions are replaced by CuII ions. Protein engineering …
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Isolation, identification and characterisation of novel actinobacteria from Zambian hot-springs
… source of novel secondary metabolites and enzymes.The biocatalytic conversion of 5-monosubstituted hydantoin derivatives to optically pure amino acids involves two reaction steps. The first step, catalysed by a hydantoinase, yields an N-carbamylamino acid intermediate, which is subsequently …
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Molecular therapy for mucopolysaccharidosis Type I
… patients included intravenous administration of recombinant enzyme upon diagnosis followed by hematopoeitic stem cell transplantation (HSCT) once a proper donor cell source is identified. Following HSCT, many patients exhibit a reduced rate of neurological deterioration. However, the potential of …
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The structure of the nitrilase from Rhodococcus Rhodochrous J1: homology modeling and three-dimensional reconstruction
… nitrilases are an important class of industrial enzymes that are found in all phyla. These enzymes are expressed widely in prokaryotes and eukaryotes. Nitrilases convert nitriles to corresponding acids and ammonia. They are used in industry as biocatalysts because of their specificity and …
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Novel Inhibitors for Isocitrate Lyase as a Potent Antitubercular Agent for Mycobacterium Tuberculosis
… not target latent TB. It has been shown that the enzyme isocitrate lyase (ICL) is essential for the survival and persistence of Mtb in latent TB. ICL plays an important role in two metabolic pathways required during TB latency, the glyoxylate and methylcitrate cycle. ICL is absent in humans, and …
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Role and regulation of glutamate dehydrogenase activity in Bacteroides fragilis bf1
… that B. fragilis produces two distinct GDH enzymes, one NAD(P)H-dependent (GdhA) and the other NADH-dependent (GdhB), their specific roles in nitrogen assimilation were not clearly resolved. In this dissertation, the physiological and molecular factors affecting the regulation of GDH …
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A TALE OF TWO METHYLATION MODIFICATIONS IN ARCHAEAL RNAs
… extended with in vitro tests. Using the COG1901 recombinant enzyme from Methanocaldococcus jannaschii (Mj1640), purified enzyme Pus10 from M. jannaschii and full-size tRNA transcripts or TØ-arm (17-mer) fragments as substrates, the sequential pathway of m1Ø54 formation in Archaea was …
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IDENTIFICATION AND ANALYSIS OF PROTEINS AND GENES RESPONSIBLE FOR MICROBIAL ANAEROBIC NITRATE-DEPENDENT IRON OXIDATION AND OVEREXPRESSION IN E. coli OF PERCHLORATE REDUCTASE
… operons, cytochromes and signal transduction enzymes in this metabolism. In an effort to further elucidate the metabolic process, a recently isolated bacterium strain Acidovorax ebreus strain TPSY capable of anaerobic nitrate-dependent Fe2+ oxidation was selected as a model organism in this …
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Microbial pathways of sulfur metabolism and colorectal cancer risk
… Transcripts were observed for all three enzymes involved in taurine metabolism: taurine:pyruvate aminotransferase (tpa), sulfoacetaldehyde sulfolyase, and dissimilatory sulfite reductase A (dsrA). Taurine metabolism was the most abundant metabolic pathway expressed by the microbe, and …