Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"recombinant antigens"”.
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Characterisation of a panel of recombinant antigens from Echinococcus granulosus and their role in the immunological follow up, after treatment, of cystic hydatid patients from Turkana, Kenya
… morphology. Antibody responses to cyst fluid antigens havepreviously been used to provide further information on follow up after treatmentand the current project explores the usefulness of a panel of novel recombinantantigens in mapping antibody responses to cyst morphological changes.Positive …
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Biochemistry: Production of recombinant Rh blood group antigens for detection of alloimmunisation
… blood group phenotyping of samples. Using recombinant antigens removes the disadvantage of the current antibody screening when using the human red blood cells, which can complicate the detection of antibody in di erent cases: in the case of multi-transfused individuals and in the case of …
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Avaliação de antígenos recombinantes no imunodiagnóstico da infecção por Strongyloides stercoralis em área endêmica para enteroparasitoses
… To improve diagnostic accuracy, recombinant antigens (rSs-NIE-1 and rSs-IR) have recently been employed in the immunodiagnosis of S. stercoralis infection. Aim: This study aimed to evaluate the sensitivity and specificity of recombinant antigens (rSs-NIE-1 and rSs-IR) and soluble …
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Identification of the factors impacting immunosorbent performance
… and multipoint attachment. For example, modified antigens of various sizes having a single binding epitope were synthesized from polymer-peptide adducts. The lysyl residues of recombinant hPC were chemically modified to reduce chemical reactivity with the matrix while maintaining avidity for the …
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Immunity surveillance of mumps and rubella: improved methods for the detection of virus-specific antibody
… IgG detection can be realised. Increasingly, recombinant antigens are being employed in ELISAs as cell culture antigens are difficult and expensive to produce, and are potentially infectious. In this study, the PinPoint Xa-1 T-Vector system was used to produce recombinant rubella virus (RV) El …
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Generierung, Charakterisierung und funktionaler Assay von Antikörpern und Antikörperfragmenten gegen die HCV Strukturproteine core, E1 und E2
… well as MS/MS analysis were used to verify the recombinant produced structure proteins. Additionally synthetic peptides were designed as antigens, which represented specific internal amino acid sequences of the native proteins. The peptide synthesis was commercially ordered. In a second step …
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Mucosal adjuvants and their mode of action in the female genital tract
… largely based on the use of highly purified or recombinant antigens, with limited immunogenicity. This has generated a need for development of potent vaccine adjuvants. Although a few adjuvants are included in the licensed vaccines, they are all administered systemically and their mode of action …
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Novel approaches to mucosal vaccine development Strategies in vaccine antigen production, construction of a novel mucosal adjuvant and studies of its mode of action
… depend in part on the efficient production of recombinant antigens produced in bacterial expression systems. To avoid current problems with the use of antibiotics to maintain expression plasmids, an E. coli strain capable of producing recombinant proteins using vectors maintained without the …
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Cyst morphology and serological variation in cystic echinococcosis patients from Turkana, Kenya
… them to gross ultrasound pathologies. Five recombinant antigens identified from literature to have different properties were purified and optimised. These were Echinococcus granulosus antigen B (EgAgB), Echinococcus granulosus elongation factor 1 beta/delta (EgEF-1β/δ), Echinococcus …
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Prokaryotic Production of Human Immunodeficiency VirusType 1 Subtype C Tat, Nef and Reverse Transcriptase andInvestigation of Antibody Responses to these proteins inHIV-1 Infected Individuals as well as Macaques Vaccinatedwith SAAVI DNA-C/C2 and SAAV
The purified Tat, Nef and RT recombinant proteins were used as antigens to investigate the prevalence of HIV-1 antibodies in sera of HIV-1 infected people. There was no reactivity in any of the 20 negative control sera in the Western blot assay. Analysis of 481 sera from HIV-1 infected individuals …
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Correlates of protective immunity in individuals who are exposed to Hepatitis C but appear uninfected
… study, in part due to the lack of appropriate recombinant antigens and assay systems. Therefore, this thesis described the development and validation of an interferon-? (IFN-?) ELISPOT assay using overlapping peptides (n=441). Using this assay, HCV-specific cellular immune responses were …
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The VH repertoire and clonal diversification of B cells in myositis and vasculitis
… in a number of disorders to a variety of self antigens but the precise cellular and molecular mechanisms resulting in pathology are largely unresolved. In some instances T cell mediated reactions are thought to be the main contributors to disease mechanisms but it is becoming increasingly …
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Epidemiologische Untersuchungen zur Toxoplasmose und Identifizierung immunogener parasitärer Antigene
Die Toxoplasmose ist eine Infektionskrankheit, die durch den Parasiten Toxoplasma gondii verursacht wird. Während eine Infektion bei den meisten immunkompetenten Menschen asymptomatisch abläuft, kann bei einer Primärinfektion in der Schwangerschaft der Parasit auf den Föten übergehen und zu einem …
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Role of Staphylococcus aureus GapC and GapB in immunity and pathogenesis of bovine mastitis
… (GAPDH) activity might be used as such antigens to induce protection against parasitic and microbial infections. Previous study in our laboratory on mastitis-causing streptococci indicates that GapC proteins of S. uberis and S. dysgalactiae have potential as vaccine antigens to protect …