Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 53 for “"recombinant DNA"”.
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The recombinant DNA case: balancing scientific and political decision-making
The unfolding of recombinant DNA, from research technique to political issue, is described. As a research technique, recombinant DNA (abbreviated rDNA) has opened up new vistas in biological and other fields of research. But its potential yet unproven hazard has created uneasy feelings toward the …
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A recombinant DNA approach to the design and synthesis of novel polysaccharides
Thesis (Sc. D.)--Massachusetts Institute of Technology, Dept. of Applied Biological Sciences, 1988.
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Development of a business plan for a recombinant DNA technology based pharmaceutical company
Thesis (M.S.)--Massachusetts Institute of Technology, Sloan School of Management, 1987.
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The Development of a New Cloning Strategy for the Biosynthetic Production of Brush-Forming Poly(Amino Acids)
… a major impact on numerous applications. Through recombinant DNA technology, there exists the potential to harness a cell's protein synthesis machinery to produce a brush-forming poly(amino acid) (or PAA) with an exactly specified amino acid sequence, thus controlling the polymer's composition at …
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Fate of genetically modified plant tissue in soil: what happens to the DNA
… are not a new technology. Advancements in recombinant DNA tools have allowed for developments in agriculture such as engineering plants for pest and disease control, drought resistance, increased nutritional value content, and production of pharmaceuticals. With these advancements, concerns …
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A Novel Mechanism for Site-Directed Mutagenesis of Large Catabolic Plasmids Using Natural Transformation
… is the process by which cells take up DNA from the surrounding medium under physiological conditions, altering the genotype in a heritable fashion. This occurs without chemical or physical treatment of the cells. Certain Acinetobacter strains exhibit a strong tendency to incorporate …
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ΜΕΛΕΤΗ ΤΩΝ ΑΠΛΟΤΥΠΩΝ ΚΑΙ ΜΕΤΑΛΛΑΓΩΝ ΤΗΣ Β-ΘΑΛΑΣΣΑΙΜΙΑΣ ΣΤΗΝ ΕΛΛΑΔΑ
… SITES (HAPLOTYPES) WAS CARRIED OUT BY RECOMBINANT DNA TECHNIQUES. THE IDENTIFICATION OF THE THALASSEMIC MUTATIONS WAS DONE IN AMPLIFIED DNA BY HYBRIDIZATION WITH A SET OF RADIOACTIVE ALIGONUCLEOTIDES. A NEW MUTATION WAS DETECTED BY SEQUENCING ANALYSIS.
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Development of a transformation protocol and cell culture system for the commercially important species of red macroalga, Gracilaria gracilis
… to be possible, a protocol for transformation of recombinant DNA into G. gracilis is required. In this study a transformation and tissue culture system for G. gracilis was developed. These tools provide the necessary groundwork for future genetic manipulation studies that are essential for …
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Identification and characterisation of the cell surface and enzymatic barriers to plasmid transformation in Corynebacterium glutamicum and related species
… been also studied using molecular biology or recombinant DNA approaches. However, a major early limitation in the application of this technology was poor transformation efficiency due to the presence of barriers which prevented entry or survival of incoming DNA. These barriers are both …
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ΜΕΛΕΤΗ ΤΩΝ ΦΥΣΙΚΩΝ ΠΛΑΣΜΙΔΙΩΝ ΤΟΥ ΣΤΕΛΕΧΟΥΣ ATCC 10988 ΤΟΥ ΑΙΘΑΝΟΛΟΠΑΡΑΓΩΓΟΥ ΒΑΚΤΗΡΙΟΥ ZYMOMONAS MOBILIS
… SIMILARLY, THEIR HOMOLOGY AGAINST TOTAL GENOMIC DNA AND PLASMID DNA OF Z. MOBILIS STRAINS ATCC 10988, NCIB 11163 AND CP4 WAS EXAMINED. PLASMID PZMO3 WAS NONHOMOLOGUS EITHER TO CHROMOSOMAL DNA OR ANYOF THE OTHER PLASMIDS THESE STRAINS HARBOURED. A SERIES OF RECOMBINANT PLASMIDS WERE ESTABLISHED …
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System analysis and metabolic engineering of biocatalytic reaction networks : application to indene bioconversion
… cellular properties through the application of recombinant DNA technology. To this point, the field has frequently been limited to methodological issues pertaining to gene introduction, expression and control by way of molecular biological techniques. While such methods are critical for the …
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Characterization of a family of cysteine rich proteins and development of a MaSp1 derived miniature fibroin
… for expression studies in bacteria. Using recombinant DNA methodology and protein expression studies, we develop a natural minifibroin that contains the highly conserved N- and C-terminal domains, along with several internal block repeats of MaSp1. We also characterize a family of small …
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DEVELOPMENT OF TRAPPING STYLE CASSETTES FOR NEW GENE TARGETING STRATEGIES
… development, precise cell culture assay, and recombinant DNA delivery will also be important. The vector design work in this PhD thesis encompasses 0.00001 % ofthese efforts but may to out to be highly relevant...
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Metabolic engineering analysis of post-burn hepatic hypermetabolism
… or introduction of new ones with the use of recombinant DNA technology. It has been used to investigate and modify intermediary metabolism in a variety of microbial organisms of biotechnological interest. An emerging area of application for metabolic engineering is medicine, in particular the …
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Overexpression of Human Aryl Hydrocarbon Receptor in <i> E.coli</i> Using Two Different Solubility Enhancing Tags
… of various AhR constructs in E.coli using recombinant DNA technology. As AhR forms inclusion bodies due to its poor solubility in the cytoplasm of the host bacteria, it is tagged as a “difficult to express” protein. Therefore, it is challenging to generate functional recombinant AhR …
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The effects of intellectual property on innovation : implications for R&D in biotechnology
… innovation. The proprietary technologies of recombinant DNA (rDNA) and Polymerase Chain Reaction (PCR) are assessed in relation to both their development and their diffusion strategies. Factors considered are: the type of IPRs, legal circumstances, the strategy regarding disclosure, whether a …
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The Curious Poisoned Weed: Poison Ivy Ecology and Physiology
… for introducing and transiently expressing recombinant DNA in poison ivy as a step towards future reverse genetic procedures.
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Biosynthesis of Cucurbita maxima trypsin inhibitor I in the methylophic yeast Pichia pastoris
… examples of squash inhibitors being produced by recombinant DNA technology. As this technique offers a relatively cheap way of producing large amounts of these proteins, further investigation is required. Problems have been experienced with the expression of disulphide-rich proteins in E. coli, …
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