Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 1825 for “"recombinant"”.
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Expression of Recombinant Chloroperoxidase
… can be used to produce fully modified and active recombinant chloroperoxidase. The expression of recombinant chloroperoxidase gene was placed under the control of a 900 bp chloroperoxidase promoter. A FLAG-epitope was fused in the N-terminal of the mature chloroperoxidase gene for identification …
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Purification of recombinant proteins
Thesis (M.S.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 1997.
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Construction of a recombinant immunotoxin
… useful immunotoxins have been produced using recombinant gene technology. In general, this involves fusion of a toxin gene with sequence encoding a variety of clinically relevant proteins or peptides. Using these techniques a recombinant immunotoxin has been engineered by fusing the genes …
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Recombinant Proteins for Biomedical Applications
… leaps in areas such nucleic acid, antibody, and recombinant protein technologies. Here we focus on the use of recombinant proteins as molecular recognition motifs, wound healing biomaterials, and agents for cell cycle pathway elucidation are discussed. The author's primary project is described in …
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Unique Emulsions based on recombinant Hydrophobins
… resulting in the availability of high amounts of recombinant hydrophobins nowadays. This study started with the physicochemical characterization of two recombinant hydrophobins, called H Star Proteins ® A and B. Both show a remarkable, time-dependent surface activity as well as a distinct …
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Purification of Native and Recombinant NPC1
… of the sterol binding properties of native and recombinant NPC1. Our purification yielded a membrane-bound 25-HC-binding protein which was purified more than 14,000-fold from rabbit liver membranes. This protein was identified as NPC1 by mass spectroscopy. We prepared recombinant human NPC1 and …
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Recombinant b alleles of Ustilago maydis
… responsible for allelic specificity, a series of recombinant alleles containing the variable region of b1 and the constant region of b2 were constructed using an in vivo recombination strategy. A haploid strain of U. maydis, carrying the b2 allele, was transformed with linear DNA containing …
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Simulating recombinant sequence date to evaluate and improve computational methods of multiple sequence alignment and recombinant identification
… sequences, and the accurate identification of recombinant sequences is particularly important in the context of downstream phylogenetics-based sequence analyses. Evaluating recombination detection methods requires the simulation of sequence data, and the training of statistical learning models …
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Characterisation of a recombinant human cysteine dioxygenase
Cysteine Dioxygenase is an enzyme that catalyses the reaction of cysteine to cysteinesulfinic acid and is thought to regulate the intracellular concentrations of its substrate. The enzyme may also be involved in the oxidation of exposed sulfidrils of protein and by implication, involved in cell …
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Production of recombinant monoclonal antibodies against HSET
HSET (Human Spleen Embryo Testis), belonged to the kinesin 14A family, is a protein required for centrosome clustering during cell division. Since cancer cells with extra-centrosomes could lead to multi-polar division, resulting in genomic instability and cell death, these cancer cells depend on …
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Purification of recombinant proteins with magnetic nanoclusters
… a new class of magnetic fluids for recovery of recombinant proteins from fermentation broth. Magnetic fluids are colloidally stable dispersions of magnetic nanoclusters in water that do not settle gravitational and moderate magnetic fields due to their small size. The magnetic nanoclusters …
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Oxidative damage to recombinant proteins during production
Since the introduction of recombinant human insulin nearly two decades ago, recombinant proteins have increasingly been utilized as therapeutic agents. In addition, expression of recombinant proteins is now a common tool used in basic research. Recombinant proteins are subject to many subtle …
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Recombinant collagen production optimization in Escherichia coli
… one plasmid contains a thermally inducible recombinant collagen gene (CLP3.1-his), and the other contains an arabinose-inducible lytic gene (bacteriophage T4 t-holin) along with the basally expressed T7 lysozyme gene from the pLysS plasmid. A methodology for the optimization of the …
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Recombinant Lactobacillus as a Vaccine Vector for HIV
… protein and evaluated the immunogenicity of the recombinant bacteria in a murine model. To evaluate the immunogenic efficacy of the novel vaccine we have assayed the serum levels of IgG and also mucosal IgA levels (fecal and vaginal washes). Antigen-specific cell mediated immune responses were …
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Overcoming long-standing challenges in recombinant protein expression
… production in E. coli, the most widely used recombinant host, has remained fundamentally the same since its inception. Here, we developed from the bottom up, a new expression platform using synthetic biology. The resulting platform reaches high protein titer across scales and allows for …
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Purification of Recombinant HIS6-GFP-Preptin Fusion Protiens
… NEB Q5 QuickChange methodology, followed by recombinant expression in Escherichia coli. The GFP-preptin fusion protein was purified using nickel affinity chromatography, followed by gel electrophoresis to confirm molecular weight and purity. The use of GFP facilitated real-time tracking of …
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Bone Properties of Recombinant Inbred Line - BXD Mouse
… of quantitative traits and gene function, BXD recombinant inbred (RI) lines mice have been used to identify genetic effects for bone density.</p> <p>For the first time, we investigated the bone properties of BXD RI mice by analyzing femur and tibia and compared their phenotypes of different …
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