Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"recognition sites"”.
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Investigation of the potential molecular recognition sites of two human vitamin C transporters.
… research is to modify the potential molecular recognition sites on the hSVCT1 and hSVCT2 genes through site directed mutagenesis. This study could therefore indicate regions on the amino acid sequences which could be fundamental for functionality for the two carriers. This knowledge would …
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Genetic analysis of lambda integrase and Escherichia coli integration host factor: Interactions with their recognition sites
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Genetic analysis of the proteins required for site-specific recombination of bacteriophage lambda: Interactions with each other and their recognition sites
… of the individual Int arm-type binding sites in $\lambda$ site-specific recombination, triple-base changes were made in each of the five Int arm-type binding sites, and then characterized through in vitro and in vivo recombination assays. These mutants demonstrated that the P1, …
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NAT2/AAP1 as a Prototypical Example of the AAP Amino Acid Transporter Gene Family: From Protein Topology to Regulation of Gene Expression
… 5' -flanking region contains several consensus recognition sites for putative transcription factors. I have now shown that NAT2 expression is nitrate and light inducible, but not circadian-regulated. Since the nitrate assimilatory enzymes are also induced by nitrate, the nitrate-inducible …
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Development of molecularly imprinted polymer (MIP)-based microfluidic gas sensors for Tetrahydrocannabinol (THC) detection
… samples. The findings demonstrated that the recognition sites of MIP properly captured THC molecules, enabling distinguishing THC from CBD, methanol, and ethanol.
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A study of protein-DNA interactions using atomic force microscopy and DNA origami
… of the detail of how proteins find their target sites and how they cleave at the sites remains unclear. Despite much progress in lab environments, where the tolerance for imprecise cutting is relatively high, in vivo treatment remains difficult. Most genome editing tools are derived from …
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Patterns in the sequence context of protein disulfide bonds
… searched for conserved patterns that may reflect recognition sites by the disulfide bond forming enzyme protein disulfide isomerase (PDI). Several methods of classifying disulfide bonds were explored, and each class was analyzed for conserved sequence patterns. To maximize the chances of finding a …
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Calix[4]pyrrole-based ion pair receptors
… permit a higher level of control over ion recognition and transport than that obtainable from simple ion binding. However, in spite of their potential applications in various fields, such as salt solublization, extraction, and membrane transport, ion pair receptors, which are able to form …
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MOLECULAR DYNAMICS SIMULATIONS OF BIOLOGICAL MACROMOLECULES: APPLICATIONS TO STRUCTURAL VACCINOLOGY AND PEPTIDE DESIGN
… where the prediction of antibody-binding sites, called epitopes, has proven challenging. The antibody-binding properties of an antigen depend on its structure and related dynamics. Aiming to predict the antibody-binding regions of a protein, we investigate a new approach based on the …
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Molecular Genetic Characterization of the Mating Pair Formation and Vegetative Replication Regions on the Agrobacterium Tumefaciens Ti Plasmid pTiC58
… found in the family Rhizobiaceae. The TraR recognition sites located in the traI-repA intergenic region affected expression of both traI-trb operon and repA. Copy number of a minimal rep plasmid and the Ti plasmid increased in the presence of TraR and AAI. We concluded that, (A) The Trb …
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Molecular recognition using nanotube-adsorbed polymer complexes
… also explored the concept of creating molecular recognition sites using polymer-SWCNT complexes. Molecular recognition is central to the design of therapeutics, chemical catalysis and sensor platforms, with the most common mechanisms involving biological structures such as antibodies[l] and …
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Chemical sensors based on swellable molecularly imprinted polymer microspheres in a hydrogel
… MAA was used as a functional monomer to form recognition sites with the template during polymerization. Poly (styrene-co-acrylonitrile) (20% w/w) was used as a stabilizer resulting in uniform, spherical microspheres with diameter ∼1.0 mum. The percentages of NIPA and crosslinker are …
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Ester-Based Synthetic Information Oligomers
… units used for synthesis, the bases used for recognition, and the sugar backbones have all been replaced. These systems have been found to form stable duplexes, so it is clear that the precise structure of these three modules is not crucial for information manipulation through a …
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Novel Approaches to Transgenesis in the Teleost Medaka (Oryzias latipes)
… and promoter fragments were flanked with the SB recognition sequences (inverted direct repeats (IR/DR)) and injected into one-cell stage medaka embryos with or without SB10 mRNA. Upon injection of a control construct, that lacks SB recognition sequences and without transposase, only 13 % of …
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Development of a Gram-type Specific Method for Detection of Bovine Mastitis Pathogens by Combining Loop-mediated Isothermal Amplification (LAMP) and Split Trehalase Technologies
… incorporated with SpoIII-specific recognition sequences was tested to verify and optimize the complementation conditions. Next, recognition sequences were introduced in Gram-type specific PCR primers, allowing for specific detection of PCR amplicons by ABSTA. We also integrated the …
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Structure-Function Relationships of Elements of the Bacteriophage f1 Genome
… viral or plasmid DNA that contains several HgaI recognition sites. Using f1 DNA, it is shown that only HgaI fragments that were originally adjacent on the genome can anneal, that infectious molecules are reassembled with high efficiency from a mixture of fragments, and that recombinant genomes …
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Forming magnetic chain with the help of biological organisms
… assembly was achieved by bioengineered recognition sites. A mCherry protein is displayed on the phage and is was used as a linker to a red binding nanobody (RBP) that is fused to the one of the proteins surrounding the magnetite crystal of a magnetosome. Both assemblies were actuated in …
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Core-Sheath Differentially Biodegradable Nanofiber Structures for Tissue Engineering
… is important in that they contain specific cell recognition sites that are capable of binding cells. Synthetic biodegradable polymers, on the other hand, can provide the necessary mechanical properties and their degradation rate can be controlled positively. When used alone, however, neither can …
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Molecular Cloning and Analysis of the Modification Genes of the KpnBl Restriction- Modification System of Klebsiella pneumoniae Strain GM236
… plasmids restricted by R+ cells contained the recognition sites. The plasmid sequences were compared using a computer program which is designed to find the DNA sequences unique to restricted plasmids. This analysis showed that the KpnBl recognition site is most likely the 14 mer TGA (7N) …
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Interaction of peptides, peptidomimetics and other drug candidates with the DI/tripeptide transport system in intestinal epithelial cells, using the in vitro caco-2 cell culture system
… series, indicating that the presence of multiple recognition sites on a molecule may override the deleterious effect of anyone change. Modifying current, poorly absorbed peptidomimetic structures to fit the proposed hypothetical model may improve oral bioavailability by increasing affinity for the …
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