Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 41 for “"receptor-mediated endocytosis"”.
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Receptor-mediated endocytosis of low density lipoproteins in aortic endothelial cells
… resembled fibroblasts in the distribution of LDL receptors on their surfaces. No binding of LDL was seen on postconfluent EC surfaces by either direct or indirect fluorescence microscopy. The patterns of AcLDL binding on postconfluent ECs resembled those on subconfluent ECs. Intracellular LDL and …
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The effect of hyperosmolarity on fluid-phase and receptor-mediated endocytosis in P388D1 macrophages
… components can be internalized by either receptor-mediated or fluid-phase endocytosis. Receptor-mediated endocytosis involves the internalization of receptor-ligand complexes into coated vesicles of about 0.1 μm in diameter. The average diameter of primary pinocytic vesicles has been …
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Pil1, an Eisosome Organizer, Plays an Important Role in the Recruitment of Synaptojanins and Amphiphysins to Facilitate Receptor-Mediated Endocytosis in Yeast
… protein Pil1 is known to be implicated in the endocytosis of Ste3, but the precise biological function of it during endocytosis is poorly understood. Here, I present data to reveal Pil1's role in receptor-mediated endocytosis. Using live cell imaging, I show that endocytic patches carrying Abp1 …
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The cellular degradation of the low density lipoprotein receptor and its ligand
… degradation of the low density lipoprotein (LDL) receptor, and its ligand, LDL, were investigated in order to clarify certain mechanistic aspects of these important processes. Long-term lymphoblastoid cell lines and cultured human skin fibroblasts were used to examine the fate of ¹²⁵I-LDL …
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Experimental and modeling studies of endosomal sorting using the epidermal growth factor/receptor system in fibroblasts
Receptor mediated endocytosis is the process by which cells internalize ligands which have specifically interacted with cell surface receptors. Cells have the ability to sort internalized ligands and their receptors to lysosomes for degradation, to recycle them back to the plasma membrane, or to …
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Antigen presentation: Influence of Cell Type and Route of Antigen Uptake
… conventional DCs and activated monocytes by receptor-mediated endocytosis, pinocytosis or phagocytosis and measured antigen uptake and presentation to MHCI and MHCII restricted T cells. We find that CD8- DCs are more efficient than any other types of antigen presenting cells tested in terms …
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Analysis of the Interaction and Functional Determinants of Arc and Its Regulation of Dynamin
… protein, Arc, facilitates endocytosis of glutamate receptors and regulates cytoskeletal organization in neuronal dendrites. Both of these functions may be mediated by its reported interaction with dynamin 2, a large (~100 kDa) GTPase that participates in receptor-mediated …
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Syntaxin 6- and Microtubule- Mediated Intracellular Trafficking Contributes to Golgi and Nuclear Translocation of Egfr
<p>Receptor-mediated endocytosis is well known for its degradation and recycling trafficking. Recent evidence shows that these cell surface receptors translocate from cell surface to different cellular compartments, including the Golgi, mitochondria, endoplasmic reticulum (ER), and the nucleus to …
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Yeast Dynamin Functions with Clathrin at the Golgi
… many proteins at diverse cellular locations for endocytosis, protein sorting, and membrane fusion and fission. Classical dynamin family proteins work in concert with clathrin to facilitate not only receptor-mediated endocytosis, but also traffic from the Golgi to endosomes. In this study, the …
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Cytokine engineering through ligand/receptor dynamics : a study on granulocyte colony-simulating factor
… chemotherapy. GCSF binds to its specific receptor (GCSFR) on bone marrow precursor cells and generates intracellular signals that cause these cells to proliferate and differentiate into mature neutrophils. However, for such cytokines that act as agonists for cell-surface receptors, these …
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Localized and disease-selective drug delivery using adhesive hydrogels for treatment of locally advanced TNBC
… TNBC is negative for estrogen and progesterone receptor, as well as for HER2, and hence it is not treatable with common endocrine treatment such as tamoxifen or Herceptin. Systemic neoadjuvant therapy has been established as the preferred therapeutic approach for locally advanced breast cancer, …
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Folate Receptor-Targeted Transplacental Delivery of Digoxin for the Treatment of Fetal Arrhythmia
… transplacental transfer of digoxin due to folate receptor-mediated endocytosis. Folate receptors are highly expressed in the placental trophoblast throughout pregnancy due to the importance of folic acid in fetal and placental development. We believe that this novel treatment strategy may increase …
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Structural characterization of interaction of K11 and K63 mixed-linkage polyubiquitin chains with the tUIMs of epsin1
… turn determines specific recognition by cellular receptors. Ubiquitylation of membrane surface receptor proteins plays a very important role in regulating receptor-mediated endocytosis as well as endosomal sorting for lysosomal degradation. Epsin1 is an endocytic adaptor protein with three tandem …
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Toxin-membrane interactions of Pasteurella multocida toxin and homologous domains
… at three stages during intoxication: 1) receptor binding to the cell surface, 2) membrane translocation, and/or 3) intracellular membrane targeting. To understand the intoxication process, it is necessary to develop an understanding of the structures, dynamics, and mechanisms through …
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The Liver as a Target Organ for the Non-Genomic Actions of Estrogen
… and translocation of the ligand. I confirm the receptor-mediated endocytosis of the ligand and identify the mitochondria as an intracellular target for the ligand. Using affinity chromatography, I isolate a protein which is identified as cholesterol ester hydrolase, a major enzyme in the …
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The disordered N-terminus of RLIP76: a hub for protein and membrane interactions
… in a number of cell processes ranging from receptor mediated endocytosis, to mitochondrial fission and metabolite transport. Despite implications in multiple signalling pathways, RLIP76 only has two defined domains, the Ral binding domain and a RhoGAP domain. The C-terminal third of RLIP76 …
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Mechanistic studies to evaluate the targeting specificity of novel RGD Micelles to the αVβ3 integrin receptor
… actively target a <strong>αVβ3</strong> integrin receptors on cancer cells or tumor endothelium. The current work is focused on mechanistic studies to evaluate the uptake of novel RGD amphiphi les with varying alkyl chain lengths (palmitic acid : Cl 6 and stearic acid: C 18) and hydrophilic …
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Mode of Entry and Survival of Salmonella Enterica Serovar Typhimurium in Trophoblast Cells
… choriocarcinoma cell lines through scavenger receptor-mediated endocytosis. Next, we observed that S.Tm failed to grow vigorously in macrophages, but replicated rapidly within epithelial and placental trophoblast cells. Further examination of intracellular localization of S.Tm indicated that …
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Regulation of the Lipid Kinase VPS34 by mTOR-Mediated UVRAG Phosphorylation
… to mediate efficient trafficking of cargo by endocytosis. In addition, VPS34 kinase activity is essential for inducing autophagy in combination with the protein kinase ULK1. Autophagy acts as a catabolic pathway that is up regulated in response to stress, but is negatively regulated by the …
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Delivery and stability of antisense oligonucleotide conjugates in vitro
… aimed to improve uptake of ODNs via mannose receptor mediated delivery, the second investigated the improved delivery of ODN conjugates via non-specific lipophilic interaction with the cell membrane. A mono-mannose phosphoramidite derivative was designed and synthesised and a mono-mannose ODN …
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